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The localisation of LH-RH neurones in the diencephalon of the domestic hen

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Summary

Nerve fibres and perikarya containing LH-RH-like material were identified immunohistochemically in the diencephalon of the domestic hen using the peroxidase-anti-peroxidase technique. Perikarya were thinly scattered in bilateral bands close to the third ventricle extending from the nucleus praeopticus paraventricularis magnocellularis, passing in front of the anterior commissure into the septal area. In this latter area, the perikarya tended to spread out laterally. A few perikarya were seen in the anterior portion of the nucleus paraventricularis magnocellularis but were not found in the infundibular nuclear complex. Fibre tracts were seen running dorso-ventrally in the preoptic area apparently associated with the lamina terminalis. Fibres, possibly nerve terminals, were found in the lamina terminalis and in the external layers of the anterior and posterior divisions of the median eminence. A large number of fibres was seen distributed throughout the infundibular nuclear complex; scattered fibres were found close to the third ventricle in the anterior hypothalamus. Extrahypothalamic fibres were also observed to project from the septal area into other parts of the telencephalon.

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Authors and Affiliations

  1. Agricultural Research Council's Poultry Research Centre, Roslin, Midlothian, Scotland

    R. J. Rling &  P. J. Sharp

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  1. R. J. Rling
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  2. P. J. Sharp
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The authors thank Mrs G. Connell for technical assistance, Mr R.K. Field for help with photography, Professor A. Arimura and Dr. H.M. Fraser for anti-LH-RH serum and Hoechst Pharmaceuticals for the gift of synthetic LH-RH

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Rling, R.J., Sharp, P.J. The localisation of LH-RH neurones in the diencephalon of the domestic hen. Cell Tissue Res. 222, 283–298 (1982). https://doi.org/10.1007/BF00213213

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