Summary
To obtain more information concerning the origin of interdigitating cells, the postnatal development and morphology of the periarteriolar lymphatic sheath in splenic white pulp of rats was investigated by light- and electron-microscopy. Special attention was paid to the ontogeny of interdigitating cells. The spleens of the animals were studied in the age range from 1 h to 28 days after birth.
The splenic white pulp of neonatal rats consists only of a few reticuloblasts, which are concentrically arranged around central arterioles. After 21 h an increase in promonocytes and monocytes was noted. Between the fifth and seventh postnatal day monocytogenic cells with a light and almost translucent cytoplasm appear, which display long cytoplasmic projections between the adjacent cells. Neighbouring lymphocytes often insert finger-like processes into the invaginated cellular membrane of these transitional forms. This intimate cellular contact is supported by zonulae occludentes. These cells represent transitional forms between monocytes and interdigitating cells.
From seven days of age onwards typical interdigitating cells were present as in adult animals. After the differentiation into an inner and outer periarteriolar lymphatic sheath, the T-cell-dependent area of splenic white pulp has attained its adult appearance and further changes are not to be expected.
On the basis of these findings, it is highly probable that interdigitating cells develop via transformation of monocytes.
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References
Blijham GH (1975) Histofysiologie van het helper T-cellen sytem in het konijn (Histophysiology of the helper-T-cell system in the rabbit). Ph. D. Thesis, Groningen, The Netherlands
Ewijk W van, Verzijden JHM, van der Kwast TH, Luijcx-Meijer SWM (1974) Reconstitution of the thymus dependent area in the spleen of lethally irradiated mice. A light and electron microscopic study of the T-cell environment. Cell Tissue Res 149:43–60
Friess A (1976) Interdigitating reticulum cells in popliteal lymph node of the rat. An ultrastructural and cytochemical study. Cell Tissue Res 170:43–60
Good RA Dalmasso AP, Martinez C, Archer OK, Pierce JC, Papermaster BW, (1962) The role of the thymus in the development of immunologic capacity in rabbits and mice. J Exp Med 116:773–782
Goos M, Kaiserling E, Lennert K, (1976) Mycosis fungoides. Model for T-lymphocyte homing in the skin? Br J Dermatol 94:221–222
Groscurth P (1980) Non-lymphatic cells in the lymph node cortex of the mouse, II. Postnatal development of the interdigitating cells and the dendritic reticular cells. Path Res Pract 169:235–254
Heusermann U, Stutte HJ, Müller-Hermelink HK (1974) Interdigitating cells in the white pulp of human spleen. Cell Tissue Res 153:415–417
Heusermann U, Zurborn K-H, Schroeder L, Stutte HJ, (1980) The origin of the dendritic reticulum cell. An experimental enzyme-histochemical and electron microscopic study on rabbit spleen. Cell Tissue Res 209:279–294
Hoefsmit ECM (1975) Mononuclear phagocytes, reticulum cells and dendritic cells in lymphoid tissues. In: Furth van R (ed) Mononuclear phagocytes in immunity, infection and pathology. Blackwell Oxford pp. 129–146
Hoefsmit ECM, Kamperdijk EWA, Balfour MB (1980) Reticulum cells and macrophages in the immune response. In: Furth van R (ed) Mononuclear phagocytes. Functional aspects. Part II, Martinus Nijhoff The Hague, pp 1809–1835
Holyoke EA, Latta JS, Mc Lean JV (1966) A study of the ultrastructure of the developing spleen of the albino rat. J Ultrastruct Res 15:87–94
Janković BD, Isaković K (1973) Structural correlates of immune microenvironment. In: Microenvironmental aspects of immunity. Plenum Press, New York, pp 1–5
Kaiserling E (1977) Non-Hodgkin-Lymphome, Ultrastruktur und Cytogenese. In: Veröffentlichungen aus der Pathologie. Vol. 105, Fischer, Stuttgart
Kaiserling E, Lennert K (1974) Die interdigitierende Retikulumzelle im menschlichen Lymphknoten. Eine spezifische Zelle der thymusabhängigen Region. Virchows Archiv (Cell Pathol) 16:51–61
Kaiserling E, Stein H, Müller-Hermelink HK (1974) Interdigitating reticulum cells in human thymus. Cell Tissue Res 155:47–55
Kamperdijk EWA, Raaymakers EM, de Leeuw JHS, Hoefsmit ECM (1978) Lymph node macrophages and reticulum cells in the immune response I. The primary response to paratyphoid vaccine. Cell Tissue Res 192:1–23
Lennert K, Stein H, Mohri N, Kaiserling E, Müller-Hermelink HK (1978) Malignant lymphomas other than Hodgkin's disease. In: Handbuch der speziellen pathologischen Anatomie und Histologie. Bd. II/3B, Springer Verlag, New York
Müller-Hermelink HK, Heusermann U, Kaiserling E, Stutte HJ (1976) Human lymphatic microecology, specifity, characterization and ontogeny of different reticulum cells in the B-cell and T-cell regions. In: Feldman M, Globerson A (eds) Advances in experimental medicine and biology. Vol. 66, Immune reactivity of lymphocytes Plenum Press New York pp 177–182
Müller-Hermelink HK, Markgraf R, Gaudecker v B, Schwarting H (1979) Ontogenetische und funktionelle Untersuchungen zur Herkunft und Charakterisierung der interdigitierenden Retikulumzellen im menschlichen Lymphknoten. Verh Dtsch Ges Path 63:659
Parrott DMV, de Sousa MAB, East J (1966) Thymus dependent areas in the lymphoid organs of neonatally thymectomized mice. J Exp Med 123:191–204
Petterson JC, Rose RJ (1968) Marginal zone and germinal center development in the spleens of neonatally thymectomized and non-thymectomized young rats. Am J Anat 123:489–500
Rausch E, Kaiserling E, Goos M (1977) Langerhans cells and interdigitating reticulum cells in the thymusdependent region in human dermatopathic lymphadenitis. Virchows Archiv (Cell Pathol) 25:327–343
Törö I, Oláh I (1966) Electron-microscopic study of guinea pig thymus. Acta Morphol Acad Sci Hung 14:75–90
Veerman AJP (1974) On the interdigitating cells in the thymus-dependent area of the rat spleen: A relation between the mononuclear phagocyte system and T-lymphocytes. Cell Tissue Res 148:247–257
Veerman AJP (1975) The postnatal development of the white pulp in the rat spleen and the onset of immunocompetence against a thymus-independent and a thymus-dependent antigen. Z Immun Forsch 150:45–59
Veldman JE (1970) Histophysiology and electron microscopy of the immune response. Part I and II. Ph. D. Thesis, Groningen, The Netherlands
Waksman BH, Arnason BG, Janković BD (1962) Role of the thymus in immune reactions in rats. III. Changes in the lymphoid organs of thymectomized rats. J Exp Med 116:187–206
Weiss L (1964) The white pulp of the spleen. The relationship of arterial vessels, reticulum and free cells in the periarterial lymphatic sheath. Bull John Hopkins Hosp 115:99–113
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This investigation was supported by the Deutsche Forschungsgemeinschaft (*He 537)
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Wiersbowsky, A., Grouls, V., Helpap, B. et al. Electron-microscopic study of the development of the periarteriolar zone in splenic white pulp of rats. Cell Tissue Res. 223, 335–348 (1982). https://doi.org/10.1007/BF01258494
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DOI: https://doi.org/10.1007/BF01258494