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Immunohistochemical identification of neurons containing corticotropin-releasing factor in the rat hypothalamus

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Summary

A specific rabbit anti-CRF serum and the immunoperoxidase technique were used to show that CRF-containing neurons are mainly distributed in the paraventricular and supraoptic nuclei of the rat hypothalamus. In addition, immunoreactive neurons are scattered in other hypothalamic regions. These neurons are 20–30 μm in diameter. From the present and previous investigations it may be concluded that the hypothalamic magnocellular nuclei, i.e., paraventricular and supraoptic, and other hypothalamic accessory nuclei, are the producing sites not only for vasopressin and oxytocin, but also for corticotropin-releasing factor.

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References

  • Amoss M, Burgus R, Blackwell R, Vale W, Fellows R, Guillemin R (1971) Purification, amino acid composition and N-terminus of the hypothalamic luteinizing hormone releasing factor (LRF) of ovine origin. Biochem Biophys Res Commun 44:205–210

    Google Scholar 

  • Barry J, Dubois MP (1973) Etude en immunofluorescence des structures hypothalamiques à compétence gonadotrope. Ann Endocrinol (Paris) 34:735–742

    Google Scholar 

  • Barry J, Dubois MP, Poulain P (1973) LRF producing cells of the mammalian hypothalamus. A fluorescent antibody study. Z Zellforsch 146:351–366

    Google Scholar 

  • Bloom FE, Battenberg ELF, Rivier J, Vale W (1982) Corticotropin releasing factor (CRF): immunoreactive neurones and fibers in rat hypothalamus. Regulatory Peptides 4:43–48

    Google Scholar 

  • Brazeau P, Vale W, Burgus R, Ling N, Butcher M, Rivier J, Guillemin R (1973) Hypothalamic polypeptide that inhibits the secretion of immunoreactive pituitary growth hormone. Science 179:77–79

    Google Scholar 

  • Bugnon C, Fellman D, Gouget A, Cardot J (1982) Corticoliberin in rat brain: immunocytochemical identification and localization of a novel neuroglandular system. Neurosci Lett 30:25–30

    Google Scholar 

  • Burgus R, Dunn TF, Desiderio D, Ward DN, Vale W, Guillemin R (1970) Characterization of ovine hypothalamic hypophysiotropic TSH-releasing factor. Nature 226:321–325

    Google Scholar 

  • Dierickx K (1980) Immunocytochemical localization of the vertebrate cyclic nonapeptide neurohypophyseal hormones and neurophysins. Int Rev Cytol 62:119–185

    Google Scholar 

  • George JM, Jacobowitz DM (1975) Localization of vasopressin in discrete areas of the rat hypothalamus. Brain Res 93:363–369

    Google Scholar 

  • Gibbs DM, Vale W (1982) Presence of corticotropin releasing factor-like immunoreactivity in hypophysial portal blood. Endocrinology 111:1418–1420

    Google Scholar 

  • Gillies G, Lowry PJ (1979a) Corticotropin releasing factor may be modulated vasopressin. Nature 278:463–464

    Google Scholar 

  • Gillies G, Lowry PJ (1979b) The relationship between vasopressin and corticotropin releasing factor. In: Jones MT, Gillham B, Dallman MF, Chattopadhyay S (eds) Interaction within the brain-pituitary-adrenocortical system, Academic Press, London New York Toronto Sydney San Francisco, pp 51–61

    Google Scholar 

  • Guillemin R, Rosenberg B (1955) Humoral hypothalamic control of anterior pituitary: a study with combined tissue culture. Endocrinology 57:599–607

    Google Scholar 

  • Hashimoto K, Murakami K, Ohno N, Kageyama J, Aoki Y, Takahara J, Ohta Z (1982a) A specific radioimmunoassay for corticotropin releasing factor (CRF) using synthetic ovine CRF. Life Sci (in press)

  • Hashimoto K, Ohno N, Aoki Y, Kageyama J, Takahara J, Ofuji T (1982b) Distribution and characterization of corticotropin-releasing factor and arginine-vasopressin in rat hypothalamic nuclei. Neuroendocrinology 34:32–37

    Google Scholar 

  • Hökfelt T, Elde R, Fuxe K, Johansson O, Ljungdhal Å, Goldstein M, Luft R, Efendic S, Nilsson G, Terenius L, Ganten D, Jeffcoate SL, Rehfeld J, Said S, de la Mora MP, Possani L, Tapia R, Teren L, Palacios R (1978) Aminergic and peptidergic pathways in the nervous system with special reference to the hypothalamus. In: Reichlin S, Baldessarini, J, Martin JB (eds) The hypothalamus, Raven Press, New York, pp 65–135

    Google Scholar 

  • Kawata M, Hashimoto K, Takahara J, Sano Y (1982a) Immunohistochemical demonstration of corticotropin releasing factor containing nerve fibers in the median eminence of the rat and monkey. Histochemistry 76:15–19

    Google Scholar 

  • Kawata M, Hashimoto K, Takahara J, Sano Y (1982b) Immunohistochemical demonstration of the localization of corticotropin releasing factor-containing neurons in the hypothalamus of mammals including primates. Anat Embryol 165:303–313

    Google Scholar 

  • Kawata M, Hashimoto K, Takahara J, Sano Y (1983) Differences in the distributional pattern of CRF-, oxytocin-, and vasopressin-immunoreactive nerve fibers in the median eminence of the rat. Cell Tissue Res 230:247–258

    Google Scholar 

  • Krieger DT, Liotta A, Brownstein MJ (1977) Corticotropin-releasing factor distribution in normal and Brattleboro rat brain, and effect of deafferentation, hypophysectomy and steroid treatment in normal animals. Endocrinology 100:227–237

    Google Scholar 

  • Lang RE, Voigt KH, Fehm HL, Pfeiffer EF (1976) Localization of corticotropin-releasing activity in the rat hypothalamus. Neurosci Lett 2:19–22

    Google Scholar 

  • Makara GB, Stark E, Karteszi M, Palkovits M, Rappy G (1981) Effects of paraventricular lesions on stimulated ACTH release and CRF in stalk-median eminence of the rat. Am J Physiol 240:E441-E446

    Google Scholar 

  • Moldow RL, Fischman AJ (1982a) Hypothalamic CRF-like immunoreactivity in the rat after hypophysectomy or adrenalectomy. Peptides 3:143–147

    Google Scholar 

  • Moldow RL, Fischman AJ (1982b) Radioimmunoassay of CRF-like material in rat hypothalamus. Peptides 3:37–39

    Google Scholar 

  • Olschowka JA, O'Donohue TL, Mueller GP, Jacobowitz DM (1982) Hypothalamic and extrahypothalamic distribution of CRF-like immunoreactive neurons in the rat brain. Neuroendocrinoloy 35:305–308

    Google Scholar 

  • Paull WK, Schöler J, Arimura A, Meyers CA, Chang JK, Chang D, Shimizu M (1982) Immunocytochemical localization of CRF in the ovine hypothalamus. Peptides 3:183–191

    Google Scholar 

  • Pelletier G, Désy L, Côté J, Lefèvre G, Vandry H, Labrie F (1982) Immunoelectron microscopic localization of corticotropin-releasing factor in the rat hypothalamus. Neuroendocrinology 35:402–404

    Google Scholar 

  • Rivier C, Brownstein M, Spiess J, Rivier J, Vale W (1982) In vitro corticotropin-releasing factor-induced scretion of adrenocorticotropin, β-endorphin, and corticosterone. Endocrinology 110:272–278

    Google Scholar 

  • Roth KA, Weber E, Barchas JD (1982) Immunoreactive corticotropin releasing factor (CRF) and vasopressin are colocalized in a subpopulation of the immunoreactive vasopressin cells in the paraventricular nucleus of the hypothalamus. Life Sci 31:1857–1860

    Google Scholar 

  • Saffran M, Schally AV (1955) The release of corticotropin by anterior pituitary tissue in vitro. Can J Biochem Physiol 33:408–415

    Google Scholar 

  • Schally AV, Redding TW, Bowers CY, Barret JF (1969) Isolation and properties of porcine thyrotropin-releasing hormone. J Biol Chem 244:4077–4088

    Google Scholar 

  • Schally AV, Arimura A, Baba Y, Nair RMG, Matsuo H, Redding TW, Debeljuk L, White WF (1971) Isolation and properties of the FSH and LH releasing hormone. Biochem Biophys Res Commun 43:393–399

    Google Scholar 

  • Spiess J, Rivier J, Rivier C, Vale W (1981) Primary structure of corticotropin-releasing factor from ovine hypothalamus. Proc Natl Acad Sci USA 78:6517–6521

    Google Scholar 

  • Tramu G, Pillez A (1982) Localisation immunohistochémique des terminaisons nerveuses à corticolibérine (CRF) dans l'éminence médiane du Cobaye et du Rat. CR Acad Sc Paris 294, Serie III, 107–114

    Google Scholar 

  • Vale W, Spiess J, Rivier C, Rivier J (1981) Characterization of a 41-residue ovine hypothalamic peptide that stimulates secretion of corticotropin and β-endorphin. Science 213:1394–1397

    Google Scholar 

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Supported by a grant (No. 56440022) from the Ministry of Education, Science, and Culture, Japan

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Kawata, M., Hashimoto, K., Takahara, J. et al. Immunohistochemical identification of neurons containing corticotropin-releasing factor in the rat hypothalamus. Cell Tissue Res. 230, 239–246 (1983). https://doi.org/10.1007/BF00213802

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