Summary
Immunohistochemistry was employed to study the development of somatostatin-containing cells in the pancreas and duodenum of rat fetuses and of 1–7 day-old newborns. Immunoreactive cells were first detected in the pancreatic islets on day 17 of gestation and in the duodenum on day 18. Somatostatin cells were numerous in the pancreas and gut at term and in early postnatal stages. The development of somatostatin-containing cells in both pancreatic islets and duodenum was not affected by either fetal hypophysectomy on day 16 or intrauterine growth retardation induced by ligature of the main uterine vessels on day 17 of gestation.
This is a preview of subscription content, log in via an institution to check access.
References
Alumets J, Sundler F, Håkanson R (1977) Distribution, ontogeny and ultrastructure of somatostatin immunoreactive cells in the pancreas and gut. Cell Tissue Res 185:465–479
Arimura A, Sato H, Dupont A, Nishi N, Schally AV (1975) Somatostatin: abundance of immunoreactive hormone in rat stomach and pancreas. Science 189:1007–1009
Bohlen P, Brazeau P, Benoit R, Ling N, Esch F, Guillemin R (1980) Isolation and amino acid composition of two somatostatin-like peptides from ovine hypothalamus: somatosta-tin-28 and somatostatin-25. Biochem Biophys Res Commun 96:725–734
Chayvialle JA, Paulin C, Dubois PM, Descos F, Dubois MP (1980) Ontogeny of somatostatin in the human gastro-intestinal tract, endocrine pancreas and hypothalamus. Acta Endocrinol 94:1–10
Dubois MP (1975) Presence of immunoreactive somatostatin in discrete cells of the endocrine pancreas. Proc Natl Acad Sci (Wash) 72:1340–1343
Dubois MP (1977) Differentiation of immunoreactive neurosecretory cells. In: Vincent JD, Kordon C (eds) Cell biology of hypothalamic neurosecretion. (CNRS Paris print.) Colloques Internationaux du CNRS 280:701–724
Dubois PM, Paulin C, Assan R, Dubois MP (1975) Human fetal pancreas: evidence for immunoreactive somatostatin in the endocrine cells. Nature (Lond) 256:731–732
Dubois PM, Paulin C, Dubois MP (1976) Gastrointestinal somatostatin cells in the human fetus. Cell Tissue Res 166:179–184
Fontaine-Pérus J, Le Lièvre C, Dubois MP (1980) Do neural crest cells in the pancreas differentiate into somatostatin-containing cells? Cell Tissue Res 213:293–299
Ghirlanda G, Bataille D, Dubois MP, Rosselin G (1978) Variations of the somatostatin content of gut, pancreas and brain in the developing rat. Metabolism 27:1167–1170
Jost A (1947) Expériences de décapitation de l'embryon de lapin. C.R. Hebd Séanc Acad Sci Paris 225:322–324
Patel YC, Amherdt M, Ito S, Orci L (1977) Somatostatin secretion from monolayer cultures of neonatal rat pancreas. Clin Res 25:683. (Abstract)
Patel YC, Amherdt M, Orci L (1979) Somatostatin secretion from monolayer cultures of neonatal rat pancreas. Endocrinology 104:676–679
Patzelt C, Tager HS, Caroll RJ, Steiner DF (1980) Identification of prosomatostatin in pancreatic islets. Proc Natl Acad Sci (Wash) 77:2410–2414
Rufener C, Amherdt M, Dubois MP, Orci L (1975) Ultrastructural immunocytochemical localization of somatostatin in rat pancreatic monolayer culture. J Histochem Cytochem 23:866–869
Schally AV, Huang WY, Chang RCC, Arimura A, Redding T, Millar RP, Hunkapiller MW, Hood LE (1980) Isolation and structure of prosomatostatin: a putative precursor from pig hypothalamus. Proc Natl Acad Sci (Wash) 77:4489–4493
Spiess J, Vale W (1980) Multiple forms of somatostatin-like activity in rat hypothalamus. Biochemistry 19:2861–2866
Wigglesworth JS (1964) Experimental growth retardation in the foetal rat. J Pathol Bact 88:1
Author information
Authors and Affiliations
Additional information
This investigation was supported by grant 003 from the INSERM (ATP n∘ 56-78-88)
Rights and permissions
About this article
Cite this article
Dupouy, J.P., Chatelain, A. & Dubois, M.P. Normal development of cells with somatostatin immunore activity in the pancreas and duodenum of the rat fetus and newborn. Cell Tissue Res. 231, 463–468 (1983). https://doi.org/10.1007/BF00222196
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00222196