Summary
The localization of radiolabeled estradiol and dihydrotestosterone was examined in the lymphatic organs of both male and female baboons. A total of 12 baboons were divided into two groups, each containing three males and three females. Each animal in one group, both males and females, was injected intravenously with 1 μg/kg body weight of 3H-estradiol while those in the second group were each injected with 1 μg/kg body weight of 3H-dihydrotestosterone. As controls, one male and one female from each group also received a dose of 100 μg/kg body weight of the corresponding unlabeled steroid. One and a half hours after the injections, the animals were sacrificed and the spleen, thymus, and inguinal lymph nodes removed and processed for autoradiography. The localization of 3H-estradiol was similar in both males and females. In the thymus fibroblasts and epithelio-reticular cells, but not thymocytes, localized 3H-estradiol. In lymph node and spleen, nonlymphoid tissue concentrated the labeled estrogen. Additionally, in the paracortical region of the lymph node, an unknown cell type was labeled with estrogen. Only one male baboon demonstrated nuclear localization of 3H-dihydrotestosterone. This was observed in the reticular cells in the spleen and lymph nodes. The same cell type in the organs of the remaining animals was unlabeled.
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References
Bardin CW, Catterall JF (1981) Testosterone: a major determinant of extragenital sexual dimorphism. Science 211:1285–1294
Castro JE (1975) Immunological effects of orchidectomy. Br J Urol 47:89–95
Cohn DA (1979) High sensitivity to androgen as a contribution factor in sex differences in the immune response. Arthritis Rheum 22:1218–1233
Dörner G, Eckert R, Hinz G (1980) Androgen-dependent sexual dimorphism of the immune system. Endokrinologie 76:112–114
Eidinger D, Garrett TJ (1972) Studies on the regulatory effects of the sex hormones on antibody formation and stem cell differentiation. J Exp Med 136:1098–1116
Ferreira A, Takahashi M, Nussenzmeig V (1977) Purification and characterization of mouse serum protein with specific binding affinity for C4 (Ss protein). J Exp Med 146:1001–1018
Gasc JM, Stumpf WE (1981) The bursa of Fabricius of the chicken embryo: localization and ontogenic evolution of sex steroid target cells. J Embryol Exp Morphol 63:225–231
Gasc JM, Sar M, Stumpf WE (1979) Androgen target-cells in bursa of Fabricius of the chick embryo: autoradiographic localization. Proc Soc Exp Biol Med 160:55–58
Gorski RA (1979) The neuroendocrinology of reproduction. An overview. Biol Reprod 20:111–127
Grossman CJ, Nathan P, Taylor BB, Sholiton LJ (1979a) Rat thymus dihydrotestosterone receptor: preparation, location and physiochemical properties. Steroids 34:539–553
Grossman CJ, Sholiton LJ, Nathan P (1979b) Rat thymic estrogen receptor. I. Preparation, location and physiochemical properties. J Steroid Biochem 11:1233–1240
Ison EJ, Sheridan PJ (1981) Autoradiography of diffusible substances. A practical approach. Am J Med Technol 47:38–42
Jensen EV, De Sombre ER (1973) Estrogen-receptor interaction. Science 182:126–134
Kalland T, Forsbery JG (1981) Natural killer cell activity and tumor susceptibility in female mice treated neonatally with diethylstilbestrol. Cancer Res 41:5134–5140
McCruden AB, Stimson WH (1981) Androgen binding cytosol receptors in the rat thymus. Physiochemical properties, specificity and localization. Thymus 3:105–117
McGill Jr HC, Sheridan PJ (1981) Nuclear uptake of sex steroid hormones in the cardiovascular system of the baboon. Circ Res 48:238–244
McGill Jr HC, Anselmo VC, Buchanan JM, Sheridan PJ (1980) The heart is a target organ for androgen. Science 207:775–777
McLusky NJ, Naftolin F (1981) Sexual differentiation of the central nervous system. Science 211:1294–1303
Morton JI, Weyant DA, Siegel BV, Golding B (1981) Androgen sensitivity and autoimmune disease. Immunology 44:661
Pearce P, Khabid BAK, Finder JW (1981) Androgen and the thymus. Endocrinology 109:1073–1077
Roubinian JR, Talal N, Greenspan JS, Goodman JR, Siiteri PK (1978) Effect of castration and sex hormone treatment on survival, anti-nucleic acid antibodies, and glomerulonephritis in NZB/NZW F1 mice. J Exp Med 147:1568–1583
Roubinian JR, Talal N, Siiteri PK, Sadakian JA (1979) Sex hormone modulation of autoimmunity in NZB/NZW mice. Arthritis Rheum 22:1162–1169
Shear HL, Roubinian JR, Gil P, Talal N (1981) Clearance of sensitized erythrocytes in NZB/ NZW mice, effects of castration and sex hormone treatment. Eur J Immunol 11:776–780
Shreffler DC (1976) The S region of the mouse major histocompatability complex (H — 2): genetic variation and functional role in complement system. Transplant Rev 32:140–167
Siiteri PK, Jones LA, Roubinian J, Talal N (1980) Sex steroids and the immune system I. Sex difference in autoimmune disease in NZB/NZW hybrid mice. J Steroid Biochem 12:425–432
Sljivic VS, Warr CW (1973) Oestrogens and immunity. Period Biol 75:231–244
Steinberg AD, Melez KA, Raveche ES, Reeves JP, Boegel WA, Smothers PA, Taurog JD, Weinlein L, Duvic M (1979) Approach to the study of the role of sex hormones in autoimmunity. Arthritis Rheum 22:1170–1176
Stumpf WE (1971) Autoradiographic techniques for localization of hormones and drugs at the cellular and subcellular level. Acta Endocrinol (Suppl) 153:205–221
Stumpf WE, Sar M (1976) Autoradiographic localization of estrogen, androgen, progestin and glucocorticosteroid in “target tissues” and “non-target tissues”. In: Receptors and mechanism of action of steroid hormones. Marcel Dekker, New York, pp 41–84
Weaker FJ, Sheridan PJ (1982) Localization of 3H-estradiol in the reproductive organs of male and female baboons. J Morphol 172:151–157
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Weaker, F.J., Sheridan, P.J. Autoradiographic localization of sex steroid hormones in the lymphatic organs of baboons. Cell Tissue Res. 231, 593–601 (1983). https://doi.org/10.1007/BF00218117
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DOI: https://doi.org/10.1007/BF00218117