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Changes in metabolism and hepatic ultrastructure induced by estradiol and testosterone in immature female Epinephelus akaara (Teleostei, Serranidae)

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Summary

Estradiol injections increase serum level of calcium, amino acid, glucose, protein, ammonia and creatinine in immature Epinephelus akaara, and also increase levels of total lipid, cholesterol, phospholipid and esterified fatty acids. Hepatic protein, glycogen and lipid concentrations also rise after estradiol treatment, and some hepatic enzymes participating in the metabolism of nitrogen, lipid and carbohydrate, show increased activity. Serum vitellogenin levels are increased. Testosterone treatment increases serum protein, total lipid, cholesterol, amino acid and ammonia levels, and also hepatic glycogen content, but in contrast to estradiol treatment, testosterone does not change serum vitellogenin, glucose, calcium, phospholipid, esterified fatty acid and creatinine levels, nor the hepatic lipid and protein content. A small number of hepatic enzymes shows an increased activity. Vitellogenic fish show biochemical changes similar to that of estradiol-treated fish, but are different from those of immature fish. Estradiol treatment induces ultrastructural changes in the hepatocytes of immature fish that are similar to those found in vitellogenic fish. These include a proliferation of rough endoplasmic reticulum and Golgi apparatus, and an increase in glycogen and lipid, all indicative of enhanced metabolic activity.

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Authors and Affiliations

  1. Department of Biochemistry, The Chinese University of Hong Kong, Hong Kong

    T. Bun Ng

  2. Department of Biology, The Chinese University of Hong Kong, Hong Kong

    Norman Y. S. Woo

  3. Department of Anatomy, The Chinese University of Hong Kong, Hong Kong

    Patrick P. L. Tam & Corinna Y. W. Au

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  1. T. Bun Ng
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  2. Norman Y. S. Woo
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  3. Patrick P. L. Tam
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  4. Corinna Y. W. Au
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Ng, T.B., Woo, N.Y.S., Tam, P.P.L. et al. Changes in metabolism and hepatic ultrastructure induced by estradiol and testosterone in immature female Epinephelus akaara (Teleostei, Serranidae). Cell Tissue Res. 236, 651–659 (1984). https://doi.org/10.1007/BF00217235

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