Summary
The ultrastructural localization of the glycoprotein D2 in rat adrenal gland was investigated using immunohistochemical methods, and D2 localization in cultures of adult bovine chromaffin cells was studied by immunofluorescence. D2 was found to be situated on nerve fibers passing through the adrenal cortex and in the medulla zone, and also on the surface of all chromaffin cells. In addition, it was strongly expressed on the surface of glial (Schwann) cells. Cortical cells were unreactive to the antiserum. In cultures, all adrenalin and noradrenalin [dopamine-β-hydroxylase (DBH)-positive] cells were surface labelled for D2. A less frequent second cell type was recognized in vitro which was DBH negative but D2 positive. Such cells were presumed to be Schwann cells. These data are discussed in terms of the developmental origin of the cells and with regard to the putative functional rôle of D2 in cell adhesion phenomena.
Similar content being viewed by others
References
Aunis D, Perrin D (1984) Chromaffin granule membrane-F-actin interactions and spectrin-like protein of subcellular organelles: a possible relationship. J Neurochem 42:1558–1569
Aunis D, Guérold B, Bader MF, Ciesielski-Treska J (1980) Immunocytochemical and biochemical demonstration of contractile proteins in chromaffin cells in culture. Neuroscience 5:2261–2277
Bader MF, Ciesielski-Treska J, Thierse D, Hesketh J, Aunis D (1981) Immunocytochemical study of microtubules in chromaffin cells in culture and evidence that tubulin is not an integral protein of the chromaffin granule membrane. J Neurochem 37:917–933
Bock E, Jorgensen OS (1975) Rat brain synaptic vesicles and synaptic plasma membranes compared by crossed immunoelectrophoresis. FEBS Lett 52:37–39
Bock E, Yavin Z, Jorgensen OS, Yavin E (1980) Nervous systemspecific proteins in developing rat cerebral cells in culture. J Neurochem 35:1297–1302
Bock E, Berezin V, Rasmussen S (1983) Characterization of the D2-cell adhesion molecule. Prot Biol Fluids 30:75–78
Goridis C, Deagostini-Bazin H, Hirn M, Hirsch MR, Rougon G, Sadoul R, Langley OK, Gombos G, Finne J (1983) Neural surface antigens during nervous system development. In: Cold Spring Harbor Symp Quant Biol Vol. 48. Cold Spring Harbor Laboratory, Cold Spring Harbor 527–537
Grumet M, Rutishauser V, Edelman GM (1982) Neural cell adhesion molecule is on muscle cells and mediates adhesion to nerve cells in vitro. Nature (Lond) 295:693–695
Hirn M, Pierres M, Deagostini-Bazin H, Hirsch MR, Goridis C (1981) Monoclonal antibody against cell surface glycoprotein of neurons. Brain Res 214:433–439
Jorgensen OS (1981) Neuronal membrane D2-protein during rat ontogeny. J Neurochem 37:939–946
Jorgensen OS, Bock E (1974) Brain specific synaptosomal membrane proteins demonstrated by crossed immunoelectrophoresis. J Neurochem 23:879–880
Jorgensen OS, Honegger P (1983) A neuronal adhesion molecule in the central nervous system: the D2-protein. Prot Biol Fluids 30:79–82
Jorgensen OS, Moller H (1980) Immunocytochemical demonstration of the D2-protein in the presynaptic complex. Brain Res 194:419–429
Jorgensen OS, Moller MH (1981) Immunocytochemical localization of the D2-protein in rat retina. Brain Res 221:15–26
Jorgensen OS, Dalouvee A, Thiery JP, Edelman GM (1980) The nervous system specific protein D2 is involved in adhesion among neuntes from cultured rat ganglia. FEBS Lett 111:39–42
Jorgensen OS, Richter-Landsberg C (1983) D2-protein in PC12 pheochromocytoma cells after nerve growth factor stimulation. Neuroscience 9:665–672
Langley OK, Ghandour MS, Gombos G, Hirn M, Goridis C (1982) Monoclonal antibodies as neural cell surface markers. Neurochem Res 7:349–362
Rasmussen S, Berezin V, Norgaard-Pedersen B, Bock E (1983) Purification of the glycoprotein D2 from fetal and adult human brain. Prot Biol Fluids 30:83–86
Sadoul R, Hirn M, Deagostini-Bazin H, Rougon G, Goridis G (1983) Adult and embryonic forms of mouse neural cell adhesion molecules have different binding properties. Nature (Lond) 304:347–349
Thiery JP, Brackenbury R, Rutishauser V, Edelman GM (1977) Adhesion among neural cells of the chick embryo. II. Purification and characterization of a cell adhesion molecule from neural retina. J Biol Chem 252:6841–6845
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Langley, O.K., Aunis, D. Ultrastructural immunocytochemical demonstration of D2-protein in adrenal medulla. Cell Tissue Res. 238, 497–502 (1984). https://doi.org/10.1007/BF00219864
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00219864