Summary
The region between the epidermis and the surface of the overlapping part of scales has been studied in two cichlid teleosts using transmission electron microscopy. In a few specimens only, numerous mineralized spherules (∼1 μm in diameter) are observed in the loose dermis and at the scale surface, and form a large part of the superficial outer limiting layer of the scale. In the loose dermis (stratum laxum) and close to the scale surface spherules are either free or included in dermal cells. When free, they are dispersed in the extracellular matrix of the dermis, among the fibrils of anchoring bundles, and fused with the scale surface. When included in cell vacuoles, they lie close to the lamina densa and to the scale surface. Steps in the formation of the mineralized spherules are only seen in the lamina densa of the basement membrane. The spherules contain needle-like mineral crystals radially orientated and an organic matrix of stippled material and dense granules, some of which form concentric lines around the centre of the spherules. The results suggest that mineralized spherules form in the lamina densa and pass through the dermis to the scale surface in which they are incorporated.
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References
Bard S, Sengel P (1984) Reconstitution of the epidermal basement membrane after enzymatic dermal-epidermal separation of embryonic mouse skin. Arch Anat Microsc 73(4):239–257
Bertin L (1944) Modifications proposées dans la nomenclature des écailles et des nageoires. Bull Soc Zool Fr 69:198–202
Castanet J (1981) Nouvelles données sur les lignes cimentantes de l'os. Arch Biol (Bruxelles) 92:1–24
David M, Slavkin H, Lyaruu D, Termine J (1983) Biosynthesis and secretion of enamel proteins during hamster tooth development. Calcif Tissue Int 35:366–371
Denèfle JP, Lechaire JP (1984) Epithelial locomotion and differentiation in frog skin cultures. Tissue Cell 16:499–517
Fach M (1935) Zur Entstehung der Fischschuppe. Z Anat Entw Gesch Leipzig 105:288–304
Luft JH (1971a) Ruthenium red and violet. I. Chemistry, purification, methods of use for electron microscopy and mechanism of action. Anat Rec 171:347–368
Luft JH (1971b) Ruthenium red and violet. II. Fine structural localization in animal tissues. Anat Rec 171:369–416
Martino LJ, Yeager VL, Taylor JJ (1979) An ultrastructural study of calcification nodules in the mineralization of the woven bone. Calcif Tissue Res 27:57–64
Meinke DK (1982) A light and scanning electron microscope study of microstructure, growth and development of the dermal skeleton of Polypterus (Pisces: Actinopterygii). J Zool Lond 197:355–382
Meunier FJ (1983) Les tissus osseux des Ostéichthyens. Structure, genèse, croissance et évolution. Thèse de Doctorat ès Sciences, Paris. Arch Doc Inst Ethnol micro-édition. Mus Nat Hist Nat SN 82-600-328, 200 pp
Meunier FJ, Gayet M, Geraudie J, Sire JY, Zylberberg L (1988) Données ultrastructurales sur la ganoine du dermosquelette des Actinoptérygiens primitifs. In: Russel J (ed) VIIth Symposium on dental morphology (in press)
Moss ML (1963) The biology of acellular teleost bone. Ann NY Acad Sci 109(1):337–350
Moss ML, Jones SJ, Piez KA (1964) Calcified ectodermal collagens of shark tooth enamel and teleost scale. Science 145:940–942
Nanci A, Bendayan M, Slavkin HC (1985) Enamel protein biosynthesis and secretion in mouse incisor secretory ameloblasts as revealed by high resolution immunocytochemistry. J Histochem Cytochem 33:1153–1160
Oosten J van (1957) The skin and scales. In: Brown ME (ed) The physiology of fishes. Academic Press, New York, pp 207–244
Schönbörner A, Boivin G, Baud CA (1979) The mineralization processes in teleost fish scales. Cell Tissue Res 202:203–212
Schultze HP (1977) Ausgangsform und Entwicklung der rhombischen Schuppen der Osteichthes (Pisces). Paläontol Z 51:152–168
Sire JY (1985) Fibres d'ancrage et couche limitante externe à la surface des écailles du Cichlidae Hemichromis bimaculatus (Téléostéen, Perciforme): données ultrastructurales. Ann Sci Nat Zool Paris 13:163–180
Sire JY (1986) Ontogenic development of surface ornamentation in the scales of Hemichromis bimaculatus (Cichlidae). J Fish Biol 28:713–724
Sire JY (1987) Structure, formation et régénération des écailles d'un poisson téléostéen, Hemichromis bimaculatus (Perciforme, Cichlidé). Thèse de Doctorat ès-Sciences, Univ. Paris VII
Sire JY, Geraudie J, Meunier FJ, Zylberberg L (1986) Participation des cellules épidermiques à la formation de la ganoine au cours de la régénération expérimentale des écailles de Calamoichthys calabaricus (Smith 1886) (Polypteridae, Ostéichthyens). C R Acad Sci Paris 303(14):625–628
Sire JY, Géraudie J, Meunier FJ, Zylberberg L (1987) On the origin of the ganoine: histological and ultrastructural data on the experimental regeneration of the scales of Calamoichthys calabaricus (Osteichthyes, Brachyopterygii, Polypteridae). Am J Anat 180(4):391–402
Stanley JR, Hawley-Nelson P, Yaan M, Martin GR, Katz SI (1982) Laminin and bullous pemphigoid antigen are distinct basement membrane proteins synthesized by epidermal cells. J Invest Dermatol 78:456–459
Zylberberg L, Meunier FJ (1981) Evidence of denticles and attachment fibres in the superficial layer of scales in two fishes: Carassius auratus and Cyprinus carpio (Cyprinidae, Teleostei). J Zool Lond 195:459–471
Zylberberg L, Geraudie J, Sire JY, Meunier FJ (1985) Mise en évidence ultrastructurale d'une couche organique entre l'épiderme et la ganoine du dermosquelette des Polypteridae. CR Acad Sci Paris III 10:517–522
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Sire, JY. Evidence that mineralized spherules are involved in the formation of the superficial layer of the elasmoid scale in cichlids Cichlasoma octofasciatum and Hemichromis bimaculatus (Pisces, Teleostei): an epidermal active participation?. Cell Tissue Res. 253, 165–172 (1988). https://doi.org/10.1007/BF00221751
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DOI: https://doi.org/10.1007/BF00221751