Summary
The influence of colchicine on the addition of 3H-galactose to the enamel protein in secretory amelloblasts of cultured germs of rat molar tooth was investigated by light- and electron-microscopic radioautography. In tooth germs cultured without colchicine, the reaction products of 3H-galactose were observed over Golgi cisternae at early chase times and then localized over the enamel with time. In tooth germs cultured with colchicine, the silver grains were seen over the Golgi cisternae, condensing granules and accumulated secretory granules. Some grains also appeared with time over the pale granular material precipitated in the intercellular space with colchicine treatment. In quantitative analysis with light microscopic radioautography, values of silver grain counts over the unit area (100 μm2) on ameloblasts and enamel of colchicine-treated tooth germs were significantly lower at both 0 min and 30 min chase after 30 min pulse than those of control tooth germs, respectively. This finding indicates that colchicine diminished the incorporation of 3H-galactose into the secretory ameloblast of cultured tooth germs. It is suggested that colchicine decreases the activity of the Golgi apparatus with regared to the addition of sugar to the synthesizing glycoprotein in the secretory ameloblast.
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References
Akita H, Kagayama M (1985) Ultrastructure of mouse incisor ameloblasts after vascular perfusion with colchicine. Cell Tissue Res 239:567–574
Akita H, Kobayashi Y, Kagayama M (1988) A histochemical study on lectin binding in the immature enamel and secretory ameloblasts of rat incisors. Tohoku J Exp Med 155:139–149
Banerjee D, Manning CP, Redman CM (1976) The in vivo effect of colchicine on the addition of galactose and sialic acid to rat hepatic serum glycoproteins. J Biol Chem 251:3887–3892
Blok J, Ginsel LA, Mulder-Stapel AA, Onderwater JJM, Daems WTh (1981) The effect of colchicine on the intracellular transport of 3H-fucose-labelled glycoproteins in the absorptive cells of cultured human small-intestinal tissue: An autoradiographical and biochemical study. Cell Tissue Res 215:1–12
Hiraoka J (1972) A holder for mass treatment of grids, adapted especially to electron staining and autoradiography. Stain Technol 47:297–301
Icarod JM, Ojeda JL, Hurle JM (1983) A quantitative study of the position of the Golgi apparatus in the developing chick myocardium. Effects of colchicine administration. Acta Anat 117:152–164
Karim A, Warshawsky H (1979) The effect of colcemid on the structure and secretory activity of ameloblasts in the rat incisor as shown by radioautography after injection of 3H-proline. Anat Rec 195:587–610
Magalhaes MC, Vitor AB, Magalhaes MM (1985) Effects of vinblastine and colchicine on the rat adrenal cortex: Morphometric and cytochemical studies. J Ultrastruct Res 91:149–158
Matsuo S, Takano Y, Wakisaka S, Ichikawa H, Nishikawa S, Akai M (1988a) Effect of colchicine on the transport of precursor enamel protein in secretory ameloblasts studied by 3H-proline radioautography in vitro. Anat Rec 221:812–822
Matsuo S, Takano Y, Wakisaka S, Ichikawa H, Nishikawa S, Itotagawa T, Akai M (1988b) Cytochemical study of the Golgi apparatus and related organelles of the secretory ameloblasts of rat molar tooth germs cultured with and without colchicine. Arch Oral Biol 33:433–441
Mizuhira V, Kurotaki M (1964) IGaku no Ayumi 49:729–733 (in Japanese)
Mizuhira V, Uchida K, Futaesaku Y (1979) Electron microscopic autoradiography. In: Mizuhira V (ed) Autoradiography. Ishiyaku Publishers, Tokyo, pp 130–227 (in Japanese)
Moe H, Mikkelsen H (1977) Light microscopical and ultrastructural observations on the effect of vinblastine on ameloblasts of rat incisors in vivo. 1. Short-term effect on secretory ameloblasts. Acta Pathol Microbiol Scand [A] 85:73–88
Moskalewski S, Thyberg J, Friberg U (1976) In vitro influence of colchicine on the Golgi complex in A- and B-cells of guinea pig pancreatic islets. J Ultrastruct Rec 54:304–317
Nakai M, Tatemoto Y, Mori H, Mori M (1985) Lectin-binding patterns in the developing tooth. Histochemistry 83:455–463
Nanci A, Uchida T, Warshawsky H (1987) The effects of vinblastine on the secretory ameloblasts: An ultrastructural, cytochemical, and immunocytochemical study in the rat incisor. Anat Rec 219:113–126
Patzelt C, Brown D, Jeanrenaud B (1977) Inhibitory effect of colchicine on amylase secretion by rat parotid glands. Possible localization in the Golgi area. J Cell Biol 73:578–593
Pavelka M, Ellinger A (1981) Effect of colchicine on the Golgi apparatus and GERL of rat jejunal absorptive cells. Ultrastructural localization of thiamine pyrophosphatase and acid phosphatase activity. Eur J Cell Biol 24:53–61
Pavelka M, Ellinger A (1983) Effect of colchicine on the Golgi complex of rat pancreatic acinar cells. J Cell Biol 97:737–748
Takuma S, Sawada T, Yanagisawa T (1982) Ultrastructural changes of secreting rat-incisor ameloblasts following administration of vincristine and vinblastine. J Dent Res 61 [Special Issue]:1472–1478
Thyberg J, Piasek A, Moskalewski S (1980) Effects of colchicine on the Golgi complex and GERL of cultured rat peritoneal macrophages and epiphyseal chondrocytes. J Cell Sci 45:42–58
Warshawsky H, Moore G (1967) A technique for the fixation and decalcification of rat incisors for electron microscopy. J Histochem Cytochem 15:542–549
Weinstock A, Leblond CP (1971) Elaboration of the matrix glycoprotein of enamel by the secretory ameloblasts of the rat incisor as revealed by radioautography after galactose-3H injection. J Cell Biol 51:26–51
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Matsuo, S., Ichikawa, H., Wakisaka, S. et al. Influence of colchicine on the addition of a sugar to the enamel protein in secretory ameloblasts of cultured germs of rat molar tooth by 3H-galactose radioautography. Cell Tissue Res 260, 521–528 (1990). https://doi.org/10.1007/BF00297232
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DOI: https://doi.org/10.1007/BF00297232