Summary
Sulfhydryl oxidase (SOx) immunoreactivity was investigated in the seminiferous epithelium of human biopsy material from the testes of 33 adult men with disturbed fertility. SOx immunoreactivity was expressed in normal seminiferous epithelium in type-A spermatogonia (27±4% of all spermatogonia) (n=4), in spermatocytes and round spermatids. Mature spermatozoa as well as Sertoli cells were unlabelled. within the interstitium, Leydig cells were immunopositive. In biopsies of oligozoospermic men showing hypospermatogenesis (n=24), an increase in labelled spermatogonia up to more than 90% was observed in biopsies, where seminiferous epithelia revealed only spermatogonia and Sertoli cells. Within the group of oligozoospermic patients there was a significant increase of labelled spermatogonia from 43±13% (>20 mill/ejaculate) (n=7) to 55±16% ( 20 and >20 mill/ejaculate) (n=6) to 68±8% (<5 mill/ejaculate) (n=11) and a significant (P=0.01) decrease of score count from 7.0±2.7 to 2.0±1.8. In this group the increase of labelled spermatogonia was correlated with sperm concentrations in the ajaculate (correlation coefficient: r=-0.6). In biopsies of azoospermic patients showing maturation arrest at the level of spermatocytes or spermatids (n=5) the percentage of labelled spermatogonia was within the range of 24% to 59%. Immunoreactivity in Sertoli cells was only found in single degenerating cells and in tubules showing Sertoli Cell Only Syndrome (SCO) without lumen formation. Sertoli cells within immature seminiferous cords were immunonegative, indicating that Sertoli cell SOx immunoreactivity is rather a sign of physiological alterations in degenerating cells than dependent on the stage of differentiation. Leydig cells did not show changes of immunoreactivity in any biopsy. It is concluded that SOx expression in spermatogonia may serve as a marker for spermatogenic efficiency.
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References
Almiron I, Chemes HX (1988) Spermatogenic onset. II. FSH modulates mitotic activity of germ and Sertoli cells in immature rats. Int J Androl 11:235–246
Aumüller G, Bergmann M, Seitz J (1991) Immunohistochemical distribution of sulfhydryl oxidase in the human testis. Cell Tissue Res 266:23–28
Baumal R, Baily D, Giwercman A, Skakkebaek N, Strats M, Marks A (1989) A novel maturation marker for human Sertoli cells. Int J Androl 12:354–359
Bergmann M (1987) Photoperiod and testicular function in Phodopus sungorus. Adv Anat Embryol Cell Biol 105:1–76
Bergmann M, Kumari M, Aumüller G, Hoffmann K, Seitz J (1990) Distribution pattern of testicular sulfhydryloxidase (SOx) immunoreactivity in the Djungarian hamster (Phodopus sungorus) during photoperiodically induced involution and recrudescence. Int J Androl 13:488–499
Bergmann M, Ochmen F, Kumari M, Aumüller G, Seitz J (1991) Immunhistochemical localization of testicular sulphydryloxidase (SOx) during sexual maturation of the Djungarian hamster (Phodopus sungorus). J Reprod Fertil 91:259–265
Chemes HX, Almiron I (1984) Spermatogenic onset: the role of FSH on the control of spermatogonial multiplication and differentiation in immature rat. Arch Biol Med Exp 17:257–265
De Kretser DM, Burger H, Hudson B (1974) The relationship between germinal cells and serum FSH levels in males with infertility. J Clin Endocrinol Metab 38:787–793
Fouquet JP, Dadoune JP (1986) Renewal of spermatogonia in the monkey (Macaca fascicularis). Biol Reprod 35:199–207
Hilscher W (1988) Prespermatogenesis and early spermatogenesis in mammals. In: Holstein AF, Leidenberger F, Hölzer KH, Bettendorf G (eds) Carl Schirren Symposium, Advances in Andrology. Diesbach, Berlin, pp 25–34
Hilscher W, Makoski HB (1968) Histologische und autoradiographische Untersuchungen zur „Präspermatogenese” und „Spermatogenese” der Ratte. Zeitschr Zellforsch 86:327–350
Holstein AF, Roosen-Runge EC, Schirren C (1988) Illustrated pathology of human spermatogenesis. Grosse, Berlin
Holstein AF, Schirren C (1983) Histological evaluation of testicular biopsies. In: Schirren C, Holstein AF (eds) Fortschritte der Andrologie, vol 8. Grosse, Berlin, pp 108–117
Jockenhövel F, Khan SA, Nieschlag E (1989) Diagnostic value of bioactive FSH in male infertility. Acta Endocrinol 121:802–810
Jockenhövel F, Khan SA, Nieschlag E (1990) Varying dose response characteristics of different immunoassays and in vitro bioassays for follicle stimulating hormone are responsible for changing ratios of biologically active to immunologically active follicle stimulating hormones. J Endocrinol 127:523–532
Johnson L (1989) Evaluation of the human testis and its age-related dysfunction. In: Burger EJ, Tardiff RG, Scialli aR, Zenick H (eds) Sperm measures and reproductive success. Progress in clinical and biological research, vol 302. Liss, New York, pp 35–67
Kancheva LS, Martinova YS, Georgiv VD (1990) Prepubertal rat Sertoli cells secrete a mitogenic factor(s) that stimulates germ and somatic cell proliferation. Mol Cell Endocrinol 69:121–127
Kumari M, Aumüller G, Bergmann M, Seitz J (1990) Stage-dependent appearance of sulfhydryl oxidase during spermatogenesis in the testis of rat and hamster: an immunohistochemical study. Histochemistry 94:356–371
Namiki M, Koide T, Okuyama A, Sonoda T, Itatani H, Miyake A, Aono T, Terada N, Matsumoto K (1984) Abnormality of testicular FSH receptors in infertile men. Acta Endocrinol 106:548–555
Oehmen F, Aumüller G, Bergmann M, Seitz J (1991) Distribution of sulfhydryloxidase (SOx) immunoreactivity in the testis of the axolotl (Ambystoma mexicanum) (Amphibia, Urodela). Tissue Cell 23:377–384
Paniagua R, Codesal J, Nistal M, Rodriguez MC, Santamaria L (1987) Quantification of cell types throughout the cycle of the human serminiferous epithelium and their DNA content. A new approach to the spermatogonial stem cell in man. Anat Embryol 176:225–230
Schulze C (1984) Sertoli cells and Leydig cells in man. Adv Anat Embryol Cell Biol 88:1–104
Schulze C (1988) Response of the human testis to long-term estrogen treatment: morphology of Sertoli cells. Leydig cells and spermatogonial stem cells. Cell Tissue Res 251:31–43
Schulze C, Schütte B (1990) Concretions in the human testis are derived from the basal lamina of seminiferous cords. Cell Tissue Res 260:1–12
Schulze W (1977) Licht-und elektronenmikroskopische Studien und den A-Spermatogonien von Männern mit intakter Spermatogenese und bei Patienten nach Behandlung mit Antiandrogenen. Andrologia 10:307–320
Seitz J, Aumüller G (1989) Secretory proteins from male accessory sex glands: isolation, biochemical and functional characterization. In: Holstein AF, Voigt KD, Grässlein D (eds) Reproductive biology and medicine. Diesbach, Berlin, pp 112–118
Seitz J, Kumari M, Aumüller G (1990) Sulfhydryl oxidase-an immunohistochemical marker for postnatal differentiation of rat testis. Anat Rec 226:91A
Van Alphen MMA, Van de Kant HJG, De Rooji DG (1988) Follicle-stimulating hormone stimulates spermatogenesis in the adult monkey. Endocrinology 123:1449–1455
Wang C, Dahl KD, Leung A, Chan SYW, Hsuch J (1987) Serum bioactive follicle-stimulating hormone in men with idiopathic azoospermia and oligospermia. J Clin Endocrinol Metab 65:629–633
WHO (1987) Laboratory manual for the examination of human semen and semen-cervical mucus interaction. Cambridge University Press, Cambridge
Ziegler H-G, Haider SG, Passia D, Hilscher W (1983) Enzymehistochemical and morphometrical studies on 214-1 dehydrogenase during the fetal and neonatal development of rat Leydig cells. Andrologia 15:392–397
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Bergmann, M., Aumüller, G., Seitz, J. et al. Sulfhydryl oxidase immunoreactivity in seminiferous tubules of infertile men. Cell Tissue Res 267, 209–214 (1992). https://doi.org/10.1007/BF00302957
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DOI: https://doi.org/10.1007/BF00302957