Abstract
The localization of albumin and transferrin was examined immunohistochemically in germ cells and Sertoli cells during rat gonadal morphogenesis and postnatal development of the testis. These proteins appeared as early as the 13th day of gestation in migrating primordial germ cells before Sertoli cell differentiation. In the fetal testis, strong immunoreactivity was only detected in the gonocytes. In the prepubertal testis, spermatogonia, primary spermatocytes, and some Sertoli cells accumulate albumin and transferrin. At puberty, different patterns of immunostaining of the germ cells were observed at the various stages of the cycle of the seminiferous epithelium. Diplotene spermatocytes at stage XIII, spermatocytes in division at stage XIV, and round spermatids at stages IV–VIII showed maximal staining. Labeling was evident in the cytoplasm of adult Sertoli cells. Albumin and transferrin staining patterns paralleled each other during ontogenesis.
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References
Boockfor FR, Schwarz LK, Derrick FC (1989) Sertoli cells in culture are heterogeneous with respect to transferrin release: analysis by reverse hemolytic plaque assay. Endocrinology 125:1128–1133
Brown WRA (1985) Immunohistochemical localization of the transferrin receptor in the seminiferous epithelium of the rat. Gamete Res 12:317–326
Cheng CY, Bardin CW (1986) Rat testicular testibumin is a glycoprotein responsive to FSH and testosterone that shares immunodeterminants with albumin. Biochemistry 25:5276–5288
Cheng CY, Grima J, Lee WM, Bardin CW (1987) The distribution of rat testibumin in the male reproductive tract. Biol Reprod 37:875–885
De Kretser DM (1990) Germ cell-Sertoli cell interactions. Reprod Fertil Dev 2:225–235
Garza MM, Schwarz LK, Bonner JM, Boockfor FR (1991) Sertoli cell function varies along the seminiferous tubule: the proportion and response of transferrin secretors differ between stage-associated tubule segments. Endocrinology 128:1869–1874
Gelly JL, Richoux JP, Grignon G (1991) Immunolocalization of some plasmatic proteins in basement membranes during earliest rat morphogenesis with special reference to the gonadal differentiation. Histochemistry 96:323–326
Holmes SD, Bucci LR, Lipshultz LI, Smith RG (1983) Transferrin binds specifically to pachytene spermatocytes. Endocrinology 113:1916–1918
Krishna A, Spanel-Borowski K (1990) Albumin localization in the testis of adult golden hamsters by use of immunohistochemistry. Andrologia 22:122–128
Morales C, Hugly S, Griswold MD (1987) Stage-dependent levels of specific mRNA transcripts in Sertoli cells. Biol Reprod 36:1035–1046
Petrie RG, Morales CR (1992) Receptor-mediated endocytosis of testicular transferrin by germinal cells of the rat testis. Cell Tissue Res 267:45–55
Segretain D, Egloff M, Gerard N, Pineau C, Jegou B (1992) Receptor-mediated and adsorptive endocytosis by male germ cells of different mammalian species. Cell Tissue Res 268:471–478
Shaha C, Cheng CY, Phillips DM, Talwar GP, Bardin CW (1988) Localization of immunoreactive testibumin in the testis and epididymis of adult rats. Int J Androl 11:547–559
Skinner MK (1990) Mesenchymal (stromal)-epithelial cell interactions in the testis and ovary which regulate gonadal function. Reprod Fertil Dev 2:237–243
Skinner MK, Griswold MD (1982) Secretion of testicular transferrin by cultured Sertoli cells is regulated by hormones and retinoids. Biol Reprod 27:211–221
Stallard BJ, Collard MW, Griswold MD (1991) A transferrin-like (hemiferrin) mRNA is expressed in the germ cells of rat testis. Mol Cell Biol 11:1448–1453
Sylvester SR, Griswold MD (1984) Localization of transferrin and transferrin receptors in rat testes. Biol Reprod 31:195–203
Wartenberg H (1989) Differentiation and development of the testes. In: Burger H, De Kretser DM (eds) The testis, 2nd edn. Raven Press, New York, pp 67–118
Wright WW, Parvinen M, Musto NA, Gunsalus GL, Phillips DM, Mather JP, Bardin CW (1983) Identification of stage-specific proteins synthesized by rat seminiferous tubules. Biol Reprod 29:257–270
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Gelly, JL., Richoux, JP. & Grignon, G. Immunolocalization of albumin and transferrin in germ cells and Sertoli cells during rat gonadal morphogenesis and postnatal development of the testis. Cell Tissue Res 276, 347–351 (1994). https://doi.org/10.1007/BF00306119
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DOI: https://doi.org/10.1007/BF00306119