Summary
The histology of the corpus cardiacum (c. card.) and the hypocerebral ganglion of Calliphora has been described from sections mainly stained with paraldehyde-fuchsin (PAF) and counterstained with Halmi's mixture. Concurrently the nervous connections of these organs with the neurosecretory system and the stomatogastric nervous system were studied.
Neurosecretory material from the medial neurosecretory cells of the brain (m.n.c.) could be traced through the cardiac-recurrent nerve, and passing through the c. card. it was seen to be abundantly present in the wall of the aorta and the two pairs of nerves leaving the c. card.-hypocerebral ganglion complex posteriorly, i.e. the aortic and the oesophageal nerves. However, in some old, fed flies a considerable amount of neurosecretory material was also observed in anastomosing branches of the cardiac-recurrent nerve inside the c. card. Thus storage of neurosecretory material originating in the m.n.c. may take place both in the aorta wall and in the c. card. This observation is relevant to the interpretation of previous experiments of E. Thomsen (1952).
The c. card. cells proper (the c.n.c.) were not stained by the PAF, although they are known to be neurosecretory.
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References
Ashhurst, D. E.: The connective tissues of insects. Ann. Rev. Entomol. 13, 45–74 (1968).
Bloch, B., E. Thomsen, and M. Thomsen: The neurosecretory system of the adult Calliphora erythrocephala. III. Electron microscopy of the medial neurosecretory cells of the brain and some adjacent cells. Z. Zellforsch. 70, 185–208 (1966).
Bowers, B., and B. Johnson: An electron microscope study of the corpora cardiaca and secretory neurons in the aphid, Myzus persicae (Sulz.). Gen. comp. Endocr. 6, 213–230 (1966).
Cazal, P.: Les glandes endocrines rétro-cérébrales des insectes. Bull. biol. France Belg., Suppl. 32, 1–227 (1948).
Day, M. F.: The homologies of the ring gland of Diptera brachycera. Ann. entomol. Soc. Amer. 36, 1–10 (1943).
Dethier, V. G.: The nerves and muscles of the proboscis of the blow fly Phormia regina in relation to feeding responses. Smiths. misc. Coll. 137, 157–174 (1959).
Dogra, G. S.: Studies on the neurosecretory system and the functional significance of NSM in the aorta wall of the bug, Dysdercus koenigii. J. Ins. Physiol. 13, 1895–1906 (1967).
Grandori, L.: Studio anatomico-histologico sul sistema neurosecretore in Musca domestica e Calliphora erythrocephala. Pubbl. Staz. zool. Napoli 24, Suppl., 50–51 (1954).
—, and E. Caré: I corpi faringei (= corpora cardiaca) in Calliphora erythrocephala Mg. adulta. Ann. Facolta Agrar., Milano 2, 1–8 (1953).
Green, G. W.: The control of spontaneous locomotor activity in Phormia regina Meigen. II. Experiments to determine the mechanism involved. J. Ins. Physiol. 10, 727–752 (1964).
Herlant-Meewis, H., and L. Paquet: Neurosécrétion et mue chez Carausius morosus Brdt. Ann. Sci. nat. Zool. 18, 163–169 (1956).
Hsiao, C., and G. Fraenkel: Neurosecretory cells in the central nervous system of the adult blowfly Phormia regina Meigen (Diptera: Calliphoridae). J. Morph. 119, 21–38 (1966).
Johansson, A. S.: Relation of nutrition to endocrine-reproductive functions in the milkweed bug Oncopeltus fasciatus (Dallas). (Heteroptera: Lygaeidae). Nytt Mag. Zool. 7, 1–132 (1958).
Johnson, B.: Neurosecretion and the transport of secretory material from the corpora cardiaca in aphids. Nature (Lond.) 196, 1338–1339 (1962).
—: A histological study of neurosecretion in aphids. J. Ins. Physiol. 9, 727–739 (1963).
—: Ultrastructure of probable sites of release of neurosecretory materials in an insect Calliphora stygia Fabr. (Diptera). Gen. comp. Endocr. 6, 99–108 (1966a).
—: Fine structure of the lateral cardiac nerves of the cockroach Periplaneta americana (L.). J. Ins. Physiol. 12, 645–653 (1966b).
—, and B. Bowers: Transport of neurohormones from the corpora cardiaca in insects. Science 141, 264–266 (1963).
Junqua, C.: Étude morphologique et histophysiologique des organes endocrines de l'Hydrocyrius columbiae Spin. (Hémiptères Belostomidés). Bull. biol. France Belg. 90, 154–162 (1956).
King, R. C., S. K. Aggarwal, and D. Bodenstein: The comparative submicroscopic cytology of the corpus allatum-corpus cardiacum complex of wild type and the adult female Drosophila melanogaster. J. exp. Zool. 161, 151–176 (1966).
Langley, P. A.: The neuroendocrine system and stomatogastric nervous system of the adult tsetsefly Glossina, morsitans. Proc. zool. Soc. Lond. 144, 415–424 (1965).
Nayar, K. K.: Studies on the neurosecretory system of Iphita limbata Stal. (Hemiptera). III. The endocrine glands and the neurosecretory pathways in the adult. Z. Zellforsch. 44, 697–705 (1956).
Normann, T. C.: The neurosecretory system of the adult Calliphora erythrocephala. I. The fine structure of the corpus cardiacum with some observations on adjacent organs. Z. Zellforsch. 67, 461–501 (1965).
-, and H. Duve: Experimentally induced release of a neurohormone influencing trehalose level in Calliphora erythrocephala (Diptera). (In press).
Palm, N. B.: Storage and excretion of vital dyes in insects. Ark. Zool. (Stockh.), Ser. II, 3, 195–272 (1952).
Possompès, B.: Recherches expérimentales sur le déterminisme de la métamorphose de Calliphora erythrocephala Meig. Arch. Zool. exp. 89, 203–364 (1953).
Scharrer, B.: The storage of neurosecretory material in the corpus cardiacum. Anat. Rec. 111, 554–555 (1951).
—: Neurosecretion XI. The effects of nerve section on the intercerebralis-cardiacum-allatum system of the insect Leucophaea maderae. Biol. Bull. 102, 261–272 (1952).
—: Neurosecretion XIII. The ultrastructure of the corpus cardiacum of the insect Leucophaea maderae. Z. Zellforsch. 60, 791–796 (1963).
—: Neurosecretion XIV. Ultrastructural study of sites of release of neurosecretory material in blattarian insects. Z. Zellforsch. 89, 1–16 (1968).
Smith, U., and D. S. Smith: Observations on the secretory processes in the corpus cardiacum of the stick insect Carausius morosus. J. Cell Sci. 1, 59–66 (1966).
Thomsen, E.: An experimental and anatomical study of the corpus allatum in the female blow-fly, Calliphora erythrocephala Meig. Vidensk. Medd. dansk naturhist. For. Kbh. 106, 319–405 (1942).
—: Functional significance of the neurosecretory brain cells and the corpus cardiacum in the female blow-fly, Calliphora erythrocephala Meig. J. exp. Biol. 29, 137–172 (1952).
—: Studies on the transport of neurosecretory material in Calliphora erythrocephala by means of ligaturing experiments. J. exp. Biol. 31, 322–333 (1954).
Thomsen, M.: Neurosecretion in some Hymenoptera. Dan. Biol. Skr. 7, 5, 1–24 (1954).
—: The neurosecretory system of the adult Calliphora erythrocephala. II. Histology of the neurosecretory cells of the brain and some related structures. Z. Zellforsch. 67, 693–717 (1965).
Wigglesworth, V.B.: Principles of Insect Physiology, 6th ed., 391–394. 1965.
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This work was supported by grants from the Carlsberg Foundation. I am grateful to Professor C. Overgaard Nielsen for laboratory facilities.
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Thomsen, M. The neurosecretory system of the adult Calliphora erythrocephala . Z. Zellforsch. 94, 205–219 (1969). https://doi.org/10.1007/BF00339357
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DOI: https://doi.org/10.1007/BF00339357