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Acidosis prevents growth hormone-induced growth in experimental uremia

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Abstract

The effects of 2 weeks of a daily injection (2 IU/day) of recombinant human growth hormone (GH) were studied in young (60-g) growing rats in two experiments. Experiment 1 was performed in uremic animals (mean plasma creatinine 65–71 μmol/l) who were either acidotic (mean bicarbonate 11.5 mmol/l) or had acidosis corrected (mean bicarbonate 26 mmol/l) by addition of sodium bicarbonate to the diet. Experiment 2 used rats with normal renal function (plasma creatinine 25 μmol/l) who were either non-acidotic but restricted to the dietary intake of uremic rats or rendered acidotic by ammonium chloride. GH induced an increase in body weight and length in nonacidotic uremic (+33% and +41%) and in non-acidotic food-restricted (+13% and +42%) rats, associated with an increased rate of protein synthesis and little change in plasma insulin-like growth factor 1 (IGF 1). In both acidotic rat groups, GH altered none of the parameters studied. Thus: (1) the presence of severe metabolic acidosis blunts the response to GH in uremic and non-uremic rats and (2) the increment of growth rate does not depend on a rise in plasma IGF 1.

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Authors and Affiliations

  1. INSERM U426, Faculté Xavier Bichat, 16 rue Henri Huchard, F-75018, Paris, France

    Claire Kleinknecht, Sâad Maniar, Xiang Zhou, Véronique Motel & Denise Laouari

  2. Hôpital Bégin, F-94160, Saint Mandé, France

    Jean-Pierre Yvert

  3. Laboratoire d'Explorations Fonctionnelles, Faculté Necker, 156 rue de Vaugirard, F-75015, Paris, France

    Michèle Dechaux

Authors
  1. Claire Kleinknecht
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  2. Sâad Maniar
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  3. Xiang Zhou
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  4. Véronique Motel
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  5. Denise Laouari
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  6. Jean-Pierre Yvert
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  7. Michèle Dechaux
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Kleinknecht, C., Maniar, S., Zhou, X. et al. Acidosis prevents growth hormone-induced growth in experimental uremia. Pediatr Nephrol 10, 256–260 (1996). https://doi.org/10.1007/BF00866751

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  • DOI: https://doi.org/10.1007/BF00866751

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