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Glomerulitis induced by cationized bovine serum albumin in the rat

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Abstract

The effects of injected native and cationized bovine serum albumin (BSA− and BSA+ respectively) were evaluated in rats which subsequently received anti-BSA. Thrombocytopenia, low creatinine clearance (Ccr), increased proteinuria, capillary swelling, mild tuft necrosis and BSA+ deposits in glomeruli resulted within 24 h of BSA+ injection. Later BSA+ produced mesangial expansion glomerular capillary wall (GCW) thickening and deposits of BSA+ accompanied by rabbit anti-BSA and rat anti-BSA which correlated well with small mesangial, subendothelial and subepithelial electron-dense granular accumuli. These latter enlarged considerably after the injection of anti-BSA. BSA− controls showed minimal or no lesions. The disappearance from the blood (t1/2) of a single dose of immune complexes (IC) prepared with chromatography-purified, radioiodinated anti-BSA-BSA− and BSA+ was determined in another group of rats. The t1/2 of BSA− anti-BSA was 42.8 h (95% confidence: 39.8–46.2) while that of BSA+ anti-BSA was 52.5 h (48.1–57.8). These results suggested that serum sickness glomerulitis developed only in rats injected with BSA+, due to in situ IC which presumably grew by accretion of foreign anti-BSA. Circulating IC may have developed and colocated with the latter, with dissociation and recombination at these sites. It is postulated that the functional-immunomorphological changes and the slow removal of cationized IC reported herein could be explained by the highly positive net charge of the injected antigen.

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Authors and Affiliations

  1. New York State Department of Health, Bureau of Adult and Gerontological Health, ESP Tower-Room 557, 12237, Albany, NY, USA

    Rodrigo E. Urizar

  2. Wadsworth Center for Laboratories and Research, 12237, Albany, NY, USA

    Andrew Reilly

  3. Pediatrics Division, Albany Medical College, 12208, Albany, NY, USA

    Rodrigo E. Urizar

  4. Internal Medicine Nephrology Division, Albany Medical College, 12208, Albany, NY, USA

    Jorge Cerda

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  1. Rodrigo E. Urizar
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  2. Jorge Cerda
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  3. Andrew Reilly
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Urizar, R.E., Cerda, J. & Reilly, A. Glomerulitis induced by cationized bovine serum albumin in the rat. Pediatr Nephrol 3, 149–155 (1989). https://doi.org/10.1007/BF00852897

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  • DOI: https://doi.org/10.1007/BF00852897

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