Summary
The activities of two key enzymes in flavonoid biosynthesis, phenylalanine ammonia-lyase (PAL, E.C. 4.3.1.5) and chalcone synthase (CHS, E.C. 2.3.1.74) were determined in the trunkwood of Robinia pseudoacacia L. The trees under investigation were cut at different times of the year (September, November, January and April). At all times PAL is active, both in the youngest wood layer (the outermost growth ring) and at the sapwood heartwood boundary. On the other hand, CHS is active exclusively in the vicinity of the heartwood boundary. The results indicate that PAL is involved both in the formation of lignin (outermost annual ring), and in flavonoid biosynthesis (heartwood boundary). Highest activity of both PAL and CHS could be measured at the sapwood heartwood boundary in the tree felled in November, indicating that heartwood formation was occurring mainly at that time. The flavonoids accumulated in the heartwood are obviously formed in situ and seem to be transported only to a minor extent — if at all — via the phloem and the ray cells to the heartwood.
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References
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254
Britsch L, Grisebach H (1985) Improved preparation and assay of chalcone synthase. Phytochem 24: 1975–1976
Chalutz E (1973) Ethylene-induced phenylalanine ammonia-lyase activity in carrot roots. Plant Physiol 51: 1033–1036
Drouet A, Weiswald N, Jay-Allemand C, Cornu D (1989) Biochemical attributes of rejuvenated hybrid walnut tree. Pentose phosphate pathway enzymes and glutamate dehydrogenase. Plant Physiol Biochem 27:259–267
Hahlbrock K, Grisebach H (1979) Enzymic controls in the biosynthesis of lignin and flavonoids. Annu Rev Plant Physiol 30: 105–130
Hahlbrock K, Scheel D (1989) Physiology and molecular biology of phenylpropanoid metabolism. Annu Rev Plant Physiol 40: 347–369
Hanson KR, Havir EA (1981) Phenylalanine ammonia-lyase. In: Conn EE, Stumpf PK (eds) The biochemistry of plants, vol VII Academic Press, London, pp 577–625
Heller W, Forkmann G (1988) Biosynthesis. In: Harborne JB (ed) The flavonoids. Chapman and Hall, London, pp 399–425
Hillis WE (1987) Heartwood and tree exudates. Springer Berlin Heidelberg New York
Hillis WE, Inoue T (1966) The formation of polyphenols in trees. III. The effect of enzyme inhibitors. Phytochem 5: 483–490
Hillis WE, Ishikura N (1970) The biosynthesis of polyphenols in tissues with low phenylalanine ammonia-lyase activity. Phytochem 9: 1517–1528
Hinderer W, Noe W, Seitz HU (1983) Differentiation of metabolic pathways in the umbel of Daucu-scarota. Phytochem 22: 2417–2420
Hrazdina G, Parsons GF (1982) Induction of flavonoid synthesizing enzymes by light in etiolated pea (Pisum sativum cv Midfreezer) seedlings. Plant Physiol 70: 506–510
Hyodo H, Kuroda H, Yang SF (1978) Induction of phenylalanine ammonia-lyase and increase in phenolics in lettuce leaves in relation to the development of russet spotting caused by ethylene. Plant Physiol 62:31–35
Jones DH (1984) Phenylalanine ammonia-lyase: regulation of its induction, and its role in plant development. Phytochem 23: 1349–1359
Riov J, Monselise SP, Kahan RS (1969) Ethylene-controlled induction of phenylalanine ammonia-lyase in citrus fruit peel. Plant Physiol 44: 631–635
Sagisaka S (1972) Decrease of glucose-6-phosphate and 6-phosphogluconate dehydrogenase activities in the xylem of Populus gelrica on budding. Plant Physiol 50: 750–755
Shain L, Hillis WE (1973) Ethylene production in xylem of Pinus radiata in relation to heartwood formation. Can J Bot 51: 1331–1335
Stafford HA, Lewis LL (1977) Interference by a phenylacetate pathway in isotopic assays for phenylalanine ammonia-lyase in leaf extracts. Plant Physiol 60: 830–834
Ziegler H (1968) Biologische Aspekte der Kernholzbildung. Holz Roh-Werkst 26:61–68
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Magel, E.A., Drouet, A., Claudot, A.C. et al. Formation of heartwood substances in the stem of Robinia pseudoacacia L.. Trees 5, 203–207 (1991). https://doi.org/10.1007/BF00227526
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DOI: https://doi.org/10.1007/BF00227526