Abstract
To investigate the effect of fructose ingestion on sorbitol and fructose 3-phosphate (F3P) in erythrocytes, we administered 50 g fructose with and without treatment with an aldose reductase inhibitor, epalrestat, to seven healthy, normal-glucose-tolerant, male volunteers aged 20–43 years. The same subjects were given 50 g glucose on another day. The sorbitol and F3P contents in their erythrocytes increased significantly, reaching peak levels at 60 min and 180 min, respectively, following fructose ingestion. On the other hand, glucose ingestion did not cause any statistically significant change in sorbitol content in their erythrocytes, although it significantly elevated their F3P content. Treatment with epalrestat had no significant effect on incremental changes in erythrocyte sorbitol and F3P content following fructose ingestion. This suggests that oral fructose may be converted directly to sorbitol and F3P in erythrocytes instead of being converted via glucose. Thus, the dietary intake of fructose may affect the concentrations of sorbitol and F3P in erythrocytes in normal men.
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References
Szwergold BS, Kappler F, Brown TR, Identification of fructose 3-phosphate in the lens of diabetic rats. Science 247:451–454, 1990
Petersen A, Szwergold BS, Kappler F, Weingarten M, Brown TR, Identification of sorbitol 3-phosphate and fructose 3-phosphate in normal and diabetic human erythrocytes. J Biol Chem 2165:17424–17427, 1990
Kawaguchi M, Fujii T, Kamiya Y, Ito J, Sakuma N, Fujinami T, Fructose 3-phosphate content of erythrocytes from patients with diabetes. Horm Metab Res 26:563, 1994
Monnier VM, Nonenzymatic glycosylation, the Maillard reaction and the aging. J Gerontol 45:B105–B111, 1990
Bucala R, Advanced glycosylation: chemistry biology, and implications for diabetes and aging. Adv Pharmacol 23:1–23, 1992
Malone JI, Knox G, Tedesco TA, Red cell sorbitol. Diabetes 29:861–864, 1980
Lundberg P, Roy S, Kuchel PW, Fructose 3-phosphate and 5-phosphoribosyl-1-pyrophosphate formation in perfused human erythrocytes: 31P NMR studies. Magn Reson Med 31:110–121, 1994
Ward JD, Baker RWR, Davis BH, Effect of blood sugar control on the accumulation of sorbitol and fructose in nervous tissues. Diabetes 21:1173–1178, 1972
Gabbay KH, The sorbitol pathway and the complications of diabetes. N Engl J Med 288:831–836, 1973
Brownlee M, Vlassara H, Cerami A, Nonenzymatic glycosylation and the pathogenesis of diabetic complications. Ann Intern Med 101:572–577, 1984
Lyons TJ, Glycation and oxidation: a role in the pathogenesis of atherosclerosis. Am J Cardiol 71:26B-31B, 1993
Jenkins DFA, Wolever TMS, Taylor RH, Barker H, Fielden H, Baldwin FM, Bowling AC, Newman HC, Jenkins AL, Goff DV, Glycemic index of foods: a physiological basis for carbohydrate exchange. Am J Clin Nutr 34:362–366, 1981
Osei K, Falko J, Bossetti BM, Holland GC, Metabolic effects of fructose as a natural sweetener in the physiologic meals of ambulatory obese patients with type II diabetes. Am J Med 83: 249–255, 1987
American Diabetes Association, Nutritional recommendations and principles for individuals with diabetes mellitus. Diabetes Care 10:126–132, 1987
Kikkawa R, Hatanaka I, Yasuda N, Kobayashi N, Shigeta Y, Terashima H, Morimura T, Tuboshima M, Effect of a new aldose reductase inhibitor, (E)-3-carboxymethyl-5-[(2E)-methyl-3-phenylpropenylidene] rhodanine (ONO-2235) on peripheral nerve disorders in streptozotocin-diabetic rats. Diabetologia 24:290–292, 1983
Terashima H, Hama K, Yamamoto R, Tsuboshima M, Kikkawa R, Hatanaka I, Shigeta Y, Effects of a new aldose reductase inhibitor on various tissues in vitro. J Pharmacol Exp Ther 229:226–230, 1983
Clements RS Jr, Morrison AD, Winegrad AI, Polyol pathway in aorta: regulation by hormones. Science 166:1007–1008, 1969
Fuller G, Parker RM, Approximate integration, in analytical geometry and calculus. Van Nostrand, Princeton, 1964, pp 367–368
Kamon N, Mabuchi H, Takeda R, Terashima H, Effects of aldose reductase inhibitor (ONO-2235) on human erythrocyte sorbitol concentrations in 75 g oral glucose tolerance tests. Horm Metab Res 23:226–229, 1990
Petersen A, Kappler F, Szwergold BS, Brown TR, Fructose metabolism in the human erythrocyte-phosphorylation to fructose 3-phosphate. Biochem J 284:363–366, 1992
McPherson JD, Shilton BH, Walton DJ, Role of fructose in glycation and cross-linking of proteins. Biochemistry 27: 1901–1907, 1988
Macdonald I, Keyser A, Pacy D, Some effects, in man, of varying the load of glucose, sucrose, fructose, or sorbitol on various metabolites in blood. Am J Clin Nutr 31:1305–1311, 1978
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Kawaguchi, M., Fujii, T., Kamiya, Y. et al. Effects of fructose ingestion on sorbitol and fructose 3-phosphate contents of erythrocytes from healthy men. Acta Diabetol 33, 100–102 (1996). https://doi.org/10.1007/BF00569417
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DOI: https://doi.org/10.1007/BF00569417