Abstract
X-ray absorption spectroscopy has been used to compare the metal coordination of the N-terminal zinc binding domain of eukaryal human transcription factor (TF) IIB to the previously reported structure of archaeal Pyrococcus furiosus (Pf) TFB. Full length and N-terminal fragments for both PfTFBand human TFIIB were cloned, expressed, and purified. The [C10H] variant of PfTFB was constructed to resemble the metal binding motif of higher eukaryal TFIIB proteins by mutating the second cysteine ligand to a histidine. All five proteins bind zinc in a 1 :1 ratio. Zn X-ray absorption spectroscopy of human TFIIB and [C10H]PfTFB mutant are consistent with ZnS3(N,O) ligation, and further suggest that the N/O ligand is an imidazole.
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Received: 11 May 1999 / Accepted: 2 February 2000
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Colangelo, C., Lewis, L., Cosper, N. et al. Structural evidence for a common zinc binding domain in archaeal and eukaryal transcription factor IIB proteins. JBIC 5, 276–283 (2000). https://doi.org/10.1007/s007750050372
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DOI: https://doi.org/10.1007/s007750050372