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Rapid separation of immunoglobulin M by immunoaffinity chromatography for detection of specific antibodies to rubella andTreponema pallidum

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Abstract

A rapid and easy method for isolating IgM from serum specimens in order to detect specific antibodies againstTreponema pallidum and rubella virus by routine serologic procedures is described. Serum IgM was isolated by immunoaffinity chromatography using anti-human IgM antibodies covalently bound to controlled-pore glass beads in a microcolumn. The final concentration of the IgM in the samples tested amounted to at least 16 % (average 32 %) of the original concentration (corresponding to a serum dilution of 1∶<8). IgG contamination did not exceed 0.38 % of the original serum concentration. The capacity of the column was stable for at least 50 absorption/ elution cycles. The new technique enables rapid and reliable detection of specific IgM by the rubella hemagglutination inhibition andTreponema pallidum hemagglutination tests.

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Authors and Affiliations

  1. Institutes of Medical Virology and Immunology, University of Heidelberg, Im Neuenheimer Feld 324, 6900, Heidelberg, FRG

    U. Huschka, J. Maier, E. W. Rauterberg & H. W. Doerr

Authors
  1. U. Huschka
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  2. J. Maier
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  3. E. W. Rauterberg
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  4. H. W. Doerr
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Huschka, U., Maier, J., Rauterberg, E.W. et al. Rapid separation of immunoglobulin M by immunoaffinity chromatography for detection of specific antibodies to rubella andTreponema pallidum . Eur. J, Clin. Microbiol. 1, 118–121 (1982). https://doi.org/10.1007/BF02014203

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  • DOI: https://doi.org/10.1007/BF02014203

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