Abstract
Thirty-one isolates ofAcinetobacter baumannii were collected from ten intensive care units of an Austrian university hospital. All isolates were typed by enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR). Two strains colonizing 13 infants in the neonatal intensive care unit were identified by ERIC-PCR. All otherAcinetobacter baumannii isolates had highly divergent ERIC-PCR patterns, despite having the same antibiogram. Thus, a hospital-wide clonal distribution, as suggested by identical antibiogram patterns, was excluded by ERIC-PCR.
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References
Bergogne-Berezin E, Towner KJ:Acinetobacter spp. as nosocomial pathogens: microbiological, clinical and epidemiological features. Clinical Microbiology Reviews 1996, 9:148–165.
Bergogne-Berezin E: The increasing significance of outbreaks ofAcinetobacter spp.: the need for control and new agents. Journal of Hospital Infection 1995, 30, Supplement: 441–452.
Lyytikainen O, Kîljag S, Härmä M, Vuopio-Varkila J: Outbreak of two multi-resistantAcinetobacter baumannii clones in a burns unit: emergence of resistance to imipenem. Journal of Hospital Infection 1995, 31: 451–454.
Levin ASS, Mendes CMF, Sinto SI, Sader HS, Scarpita SRM, Rodrigues E, Sauaia N, Boulos M: An outbreak of multiresistantAcinetobacter baumannii in a university hospital in Sao Paulo, Brazil. Infection Control and Hospital Epidemiology 1996, 17: 366–368.
Reboli AC, Houston ED, Monteforte JS, Wood CA, Hamill RJ: Discrimination of epidemic and sporadic isolates ofAcinetobacter baumannii by repetitive element PCR-mediated DNA fingerprinting. Journal of Clinical Microbiology 1994, 32: 2635–2640.
Dijkshoorn J, Aucken H, Gerner-Smidt P, Janssen P, Kaufmann M, Garaizar J, Ursing J, Pitt TL: Comparison of outbreak and nonoutbreakAcinetobacter baumannii strains by genotypic and phenotypic methods. Journal of Clinical Microbiology 1996, 34: 1519–1525.
Ehrenstein B, Bernards AT, Dijkshorn L, Gerner-Smidt P, Towner KJ, Bouvet PJM, Daschner FD, Grundmann H:Acinetobacter species identification by using tRNA spacer fingerprinting. Journal of Clinical Microbiology 1996, 34: 2414–2420.
Marcos MA, Jimenez de Anta MT, Vila J: Correlation of six methods for typing nosocomial isolates ofAcinetobacter baumannii. Journal of Medical Microbiology 1995, 42: 328–335.
Versalovic J, Koeuth T, Lupski JR: Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Research 1991, 19: 6823–6831.
Bouvet PJM, Grimont PA: Taxonomy of the genusAcinetobacter. International Journal of Systematic Bacteriology 1986, 36: 228–240.
Current protocols in molecular biology. Ansubel FM, Brent R, Kingston RE, Moore DD (ed): Wiley & Sons, Boston 1990, p. 2.4.1.
Struelens MJ, Carlier E, Maes N, Serruys E, Quint WGV, van Belkum A: Nosocomial colonization and infection with multiresistantAcinetobacter baumannii: outbreak delineation using DNA macrorestriction analysis and PCR-fingerprinting. Journal of Hospital Infection 1993, 25: 15–32.
Liu PYF, Lau YJ, Hu BS, Shyr JM, Shi ZY, Tsai WS, Lin YH, Tseng CY: Analysis of clonal relationships among isolates ofShigella sonnei by different molecular typing methods. Journal of Clinical Microbiology 1995, 33: 1779–1783.
Gilson E, Clement JM, Brutlag D, Hofnung M: A family of dispersed repetitive extragenic palindromic sequences inE. coli. EMBO Journal 1984, 3: 1417–1421.
Snelling AM, Gerner-Smidt P, Hawkey PM, Heritage J, Parnell P, Porter C, Bodenham AR, Inglis T: Validation of use of whole cell repetitive palindromic sequence-based PCR (REP-PCR) for typing strains belonging to theAcinetobacter calcoaceticus-Acinetobacter baumannii complex and application of the method to the investigation of hospital outbreaks. Journal of Clinical Microbiology 1995, 34: 1193–1202.
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Presterl, E., Nadrchal, R., Winkler, S. et al. Molecular typing ofAcinetobacter baumannii from ten different intensive care units of a university hospital. Eur. J. Clin. Microbiol. Infect. Dis. 16, 740–743 (1997). https://doi.org/10.1007/BF01709255
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DOI: https://doi.org/10.1007/BF01709255