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Relaxivity and molecular dynamics of spin labeled polysaccharides

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Abstract

Synthetic methods are described leading to polysubstituted spin labeled polysaccharides (dextran 70, 110, 200, and arabinogalactan), which are potential contrast agents in magnetic resonance imaging. The nitroxyl moieties bound to a polysaccharide yield relaxivity enhanced by a factor of 1.6 (at 0.47 T and 37°C) compared to that of the small hydrophilic radicals. An EPR study at variable temperature and viscosity (by adding sucrose) reveals the presence of two mobile bound spin labels. The less mobile ones are characterized by an order parameterS≅0.6 and a macromolecular rotational correlation time of ∼1.6 ns at 20°C for the substituted dextrans. Substitution rate effects are observed and might be related to conformational changes.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Laboratory of Medicinal Chemistry, Catholic University of Louvain, B-1200, Brussels, Belgium

    B. Gallez, J. L. De Keyser, V. Lacour & P. Dumont

  2. Laboratory of Inorganic, Analytical, and Nuclear Chemistry, Catholic University of Louvain, B-1348, Louvain-la-Neuve, Belgium

    R. Debuyst & F. Dejehet

  3. Department of Radiology, Cliniques Universitaires Saint-Luc, B-1200, Brussels, Belgium

    R. Demeure

Authors
  1. B. Gallez
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  2. R. Debuyst
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  3. F. Dejehet
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  4. J. L. De Keyser
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  5. V. Lacour
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  6. R. Demeure
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  7. P. Dumont
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Address for correspondence: Laboratory of Medicinal Chemistry, CMFA/UCL 7340, Avenue E. Mounier, B-1200 Brussels, Belgium. Additional reprints of this chapter may be obtained from the Reprints Department, Chapman & Hall, One Penn Plaza, New York, NY 10119.

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Gallez, B., Debuyst, R., Dejehet, F. et al. Relaxivity and molecular dynamics of spin labeled polysaccharides. MAGMA 2, 61–68 (1994). https://doi.org/10.1007/BF01709801

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