Abstract
Introduction: Multifocal electroretinography (MF-ERG) is widely used in the detection of local retinal dysfunction. However, the position of the stimulus on the retina and the stability of fixation are usually not directly accessible. Thus, devices have been designed for a continuous fundus visualization during recording. Methods: MF-ERGs were recorded with a RetiScanTM system connected to two different Scanning-laser ophthalmoscopes (SLOs) that use either a red (633 nm) or green (415 nm) laser for stimulation, and a VERISTM 4 system connected to a piggyback stimulator prototype that added the stimulus to the optical pathway of the SLO by means of a wavelength-sensitive mirror. Fundus visualization was achieved with the infrared lasers of the SLOs (780 and 835 nm). Results: The most extensive study so far with a green laser stimulus in a cat model of retinal degeneration demonstrated the capability of the device to detect retinal landmarks and the different stages of degeneration. Also, the advantages of exactly reproducible stimulus positioning for averaging within and comparison between disease groups became apparent. The results with the same setup in transgenic mice suggest a pure cone origin of the responses. In humans, recordings show that fixation is sufficiently good in most subjects. It is not clear yet whether red or green laser stimulation (or both) is preferable. The results with the prototype were very similar to the MF-ERGs obtained with a standard CRT screen. Conclusions: All three devices allowed us to record MF-ERGs with continuous fundus monitoring. Although further refinements are necessary, it is obvious that fundus controlled methods will improve the reliability of MF-ERG in future research on glaucoma, transplantation studies, and evaluation of gene therapy.
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Seeliger MW, Kretschmann UH, Rüther KW, Apfelstedt-Sylla E, Zrenner E. Multifocal electroretinography in Retinitis Pigmentosa. Am J Ophthalmol 1998; 125: 214–226.
Kretschmann U, Seeliger M, Ruether K, Zrenner E. Spatial cone activity distribution in diseases of the posterior pole determined by multifocal electroretinography. Vision Res 1998; 38: 3817–28.
Kretschmann U, Seeliger M, Rüther K, Usui T, Apfelstedt-Sylla E, Zrenner E. Multifocal electroretinography in patients with Stargardt's macular dystrophy. Br J Ophthalmol 1998; 82: 267–75.
Sutter EE, Tran D. The field topography of ERG components in man-I. The photopic luminance response. Vision Res 1992; 32: 433–46.
Hood DC, Seiple W, Holopigian K, Greenstein V, Carr RE. A comparison of the components of the multi-focal and full-field ERGs. Vis Neurosci 1997; 14: 533–44.
Seeliger MW, Narfström K, Scholz S, Tornow R, Miliczek K, Schwahn H, Zrenner E. Analysis of retinal function in animals using multifocal ERG with scanning laser ophthalmoscope (SLO) stimulation. In: Kremlacek J, Kuba M, Kubova Z. Abstract book of the 36th ISCEV symposium, ATD Press, Hradec Kralove 1998; p 79.
Seeliger MW, Kretschmann UH, Apfelstedt-Sylla E, Zrenner E. Implicit Time Topography of Multifocal Electroretinograms. Invest Ophthalmol Vis Sci 1998; 39: 718–723.
Humphries MM, Rancourt D, Farrar GJ, Kenna P, Hazel M, Bush RA, Sieving PA, Sheils DM, McNally N, Creighton P, Erven A, Boros A, Gulya K, Capecchi MR, Humphries P. Retinopathy induced in mice by targeted disruption of the rhodopsin gene. Nature Genetics 1997; 15: 216–9.
Biel M, Seeliger MW, Pfeifer A, Kohler K, Gerstner A, Ludwig A, Jaissle g, Fauser S, Zrenner E, Hofmann F. Selective loss of cone function in mice lacking the cyclic nucleotide-gated channel CNG3. Proc Natl Acad Sci USA 1999; 96: 7553–7557.
Bennett J, Maguire AM, Cideciyan AV, et al. Stable transgene expression in rod photoreceptors after recombinant adeno-associated virus-mediated gene transfer to monkey retina. Proc Natl Acad Sci USA 1999; 96: 9920–25.
Hood DC, Holopigian K, Greenstein VC, Seiple W, Carr RE, Sutter EE. Assessment of local retinal function in patients with retinitis pigmentosa using the multi-focal ERG technique. Vision Res 1998; 38: 163–79.
Seeliger MW, Narfström K. Functional assessment of the regional distribution of disease in a cat model of hereditary retinal degeneration. Invest Ophthalmol Vis Sci 2000; 41: 1998–2005.
Narfström K. Progressive retinal atrophy in the Abyssinian cat. Clinical characteristics. Invest Ophthalmol Vis Sci 1985; 26: 193–200.
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Seeliger, M.W., Narfström, K., Reinhard, J. et al. Continuous monitoring of the stimulated area in multifocal ERG. Doc Ophthalmol 100, 167–184 (2000). https://doi.org/10.1023/A:1002731703120
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DOI: https://doi.org/10.1023/A:1002731703120