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Neue Möglichkeiten in der immunologischen Diagnostik der Treponemen-Infektion (Syphilis)

New possibilities in the immunological diagnosis of treponema infection (syphilis)

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Zusammenfassung

Es wird über ein Verfahren zum Nachweis treponemen-spezifischer IgM-Antikörper berichtet. Dabei werden die Antikörper unter Verwendung eines FITC-markierten Anti-Human-IgM-Serums vom Kaninchen im Patientenserum direkt — oder nach Gel-Diffusion des Serums über Sephadex G 200 — fluoreszenz-optisch erfaßt. Die an den Serumproben von 609 Patienten mit unbehandelter bzw. ausreichend behandelter Syphilis erhobenen Befunde werden mitgeteilt. Sie zeigen, daß die Methodik zur Früherfassung einer Treponemen-Infektion beim Erwachsenen geeignet ist. Darüber hinaus wird dargestellt, daß das Testergebnis die Beurteilung des Effektes einer spezifischen, antibiotischen Behandlung der Infektion zu ermöglichen scheint.

Summary

A report is given on a technique for detection of specific IgM antibodies against Treponema. Using an FITC-labelled antihuman IgM serum from rabbits the antibodies are identified in the patient's serum by fluorescence either directly or after gel-diffusion of the serum through sephadex G 200. The findings in serum samples from 609 patients with either untreated or adequately treated syphilis are presented. These show that the method is suitable for the early recognition of a Treponema infection. It is also pointed out that the results of the test would appear to enable the assessment of the effects of a specific antibiotic therapy of the infection.

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Authors and Affiliations

  1. Mit Unterstützung der Deutschen Forschungsgemeinschaft, Bonn-Bad Godesberg, Germany

    F. Müller & P. L. Loa

  2. Abteilung Serologie, Hygienisches Institut, Gorch-Fock-Wall 15/17, D-2000, Hamburg 36

    F. Müller &  F. Müller

Authors
  1. F. Müller
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  2. P. L. Loa
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  3. F. Müller
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Additional information

In Anlehnung an einen Vortrag auf der 1. Tagung der Arbeitsgemeinschaft dermatologische Forschung (ADF), November 1973, Düsseldorf.

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Müller, F., Loa, P.L. & Müller, F. Neue Möglichkeiten in der immunologischen Diagnostik der Treponemen-Infektion (Syphilis). Infection 2, 127–131 (1974). https://doi.org/10.1007/BF01642231

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  • DOI: https://doi.org/10.1007/BF01642231

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