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Quantification of total IgM and IgG levels in rat sera by a sandwich ELISA technique

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Abstract

Routine determination of IgG and IgM levels is likely to be included in future international immunotoxicity assessment guidelines with the rat as the required animal species. Only a few historical data are available on these two parameters in the rat. We describe a quantitative ELISA sandwich method using commercially available purified rat immunoglobulin as standards and provide the normal range for IgG and IgM values in healthy untreated 10 ± 2 to 30 ± 3 weekold Sprague-Dawley rats. Our data indicate that both IgM and IgG levels increase gradually with age. In addition, we determined IgG values in rats hyperimmunised with protamine, lysozyme and casein.

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Authors and Affiliations

  1. Rhône-Poulenc Rorer SA, Drug Safety Department, Vitry Cedex, France

    D. Salauze, V. Serre & C. Perrin

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  1. D. Salauze
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  2. V. Serre
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  3. C. Perrin
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Salauze, D., Serre, V. & Perrin, C. Quantification of total IgM and IgG levels in rat sera by a sandwich ELISA technique. Comp Haematol Int 4, 30–33 (1994). https://doi.org/10.1007/BF00368263

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  • DOI: https://doi.org/10.1007/BF00368263

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