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Anti-transfer RNA antibodies in two patients with pulmonary fibrosis, Raynaud's phenomenon and polyarthritis

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Abstract

Patient W.S. (a 61-year-old woman) and patient T.M. (a 41-year-old man) developed recurrent fevers, polyarthritis, Raynaud's phenomenon and interstitial pulmonary fibrosis without apparent polymyositis. From HeLa cell extracts, sera from both patients immunoprecipitated all species of intact and deproteinised tRNAs. To identify the antibody binding site more precisely, tRNAs transcribed in vitro from clonedEscherichia coli tRNA genes and various mutants were prepared and used as antigens for immunoprecipitation. When the TψC loop, or the D loop were deleted, such mutants were not bound by both sera, suggesting that the D and TψC loops were required for antibody binding. Abrogation of tRNA binding occurred when18G of tRNATrp was replaced with18A to break the tertiary L-shape structure of tRNA. These results strongly suggest that sera from W.S. and T.M. recognise the tertiary conformation of L-shaped tRNA which is constructed with both D and TψC loops. These autoantibodies may also serve as a marker for a new subset of patients with connective tissue diseases that is distinct from anti-aminoacyl-tRNA synthetase syndrome.

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References

  1. Lamon EW, Bennet JC. Antibodies to ribosomal ribonucleic acid (rRNA) in patients with systemic lupus erythematosus (SLE). Immunology 1970;19:439–42.

    PubMed  Google Scholar 

  2. Wilusz J, Keene JD. Autoantibodies specific for U1 RNA and initiator methionine tRNA. J Biol Chem 1986;261:5467–72.

    PubMed  Google Scholar 

  3. Van Venrooij WJ, Hoet R, Castrop J, Hageman B, Mattaj I, van de Putte LBA. Anti-(U1) small nuclear RNA antibodies in anti-small nuclear ribonucleoprotein sera from patients with connective tissue diseases. J Clin Invest 1990;86:2154–60.

    PubMed  Google Scholar 

  4. Bunn CC, Bernstein RN, Mathews MB. Autoantibodies against alanyl-tRNA synthetase and tRNAAla coexist and are associated with myositis. J Exp Med 1986;163:1281–91.

    PubMed  Google Scholar 

  5. Sri-Widada J, Graafland H, Dietz R, Brunel C, Cathala G, Jeanteur P. A human autoimmune antibody specifically recognizing initiator methionine tRNA from yeast and higher eukaryotes. Biochem Biophys Res Commun 1986;139:275–80.

    PubMed  Google Scholar 

  6. Matsumura M, Ohosone Y, Miyachi K, Akizuki M, Matsuoka Y, Irimajiri S, et al. Novel autoantibodies directed against the common tertiary configuration of transfer RNA in a patient with interstitial lung disease. Arthritis Rheum 1995;39:1308–12.

    Google Scholar 

  7. Mimori T, Hinterberger M, Pettersson I, Steitz JA. Autoantibodies to the U2 small nuclear ribonucleoprotein in a patient with scleroderma-polymyositis overlap syndrome. J Biol Chem 1984; 259:560–5.

    PubMed  Google Scholar 

  8. Shimizu M, Asahara H, Tamura K, Hasegawa T, Himeno H. The role of anticodon bases in the discriminator nucleotide in the recognition of someE.coli tRNAs by their aminoacyl-tRNA-synthetases. J Mol Evol 1992;35:436–43.

    PubMed  Google Scholar 

  9. Targoff IN, Arnett FC. Clinical manifestations in patients with antibody to PL-12 antigen (alanyl-tRNA synthetase). Am J Med 1990;88:241–51.

    PubMed  Google Scholar 

  10. Bernstein RM, Morgan SH, Chapman J, Bunn CC, Mathews MB, Turner-Warwick M, Hughes GRV. Anti-Jo-1 antibody: a marker for myositis with interstitial lung disease. Br Med J 1984; 289:151–2.

    Google Scholar 

  11. Yoshida S, Akizuki M, Mimori T, Yamagata H, Inada S, Homma M. The precipitating antibody to an acidic nuclear protein antigen, the Jo-1, in connective tissue diseases. A marker for a subset of polymyositis with interstitial pulmonary fibrosis. Arthritis Rheum 1983;26:604–11.

    PubMed  Google Scholar 

  12. Targoff IN: Inflammatory muscle disease. In: Cannon GW, Zimmerman GA, editors. The lung in rheumatic diseases. New York: Marcel Dekker, 1990:303–28.

    Google Scholar 

  13. Sato T, Uchiumi T, Arakawa M, Kominami R. Serological association of lupus autoantibodies to a limited functional domain of 27S ribosomal RNA and to the ribosomal proteins bound to the domain. Clin Exp Immunol 1994;98:35–9.

    PubMed  Google Scholar 

  14. Hoet RMA, Koornneef I, de Rooij DJ, van de Putte LB, van Venrooij WJ. Changes in anti-U1 RNA antibody levels correlate with disease activity in patients with systemic lupus erythematosus overlap syndrome. Arthritis Rheum 1992;35:1202–10.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Departments of Internal Medicine, Keio University School of Medicine, 35-Shinanomachi, Shinjuku-ku, Tokyo, Japan

    Y. Ohosone, M. Matsumura & T. Mimori

  2. Yokosuka National Hospital, Yokosuka, Japan

    J. Chiba & S. Nagaoka

  3. Kawasaki Municipal Hospital, Kawasaki, Japan

    Y. Matsuoka & S. Irimajiri

Authors
  1. Y. Ohosone
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  2. M. Matsumura
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  3. J. Chiba
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  4. S. Nagaoka
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  5. Y. Matsuoka
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  6. S. Irimajiri
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  7. T. Mimori
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Ohosone, Y., Matsumura, M., Chiba, J. et al. Anti-transfer RNA antibodies in two patients with pulmonary fibrosis, Raynaud's phenomenon and polyarthritis. Clin Rheumatol 17, 144–147 (1998). https://doi.org/10.1007/BF01452262

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  • DOI: https://doi.org/10.1007/BF01452262

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