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Optical and radiographic appearance of thin ground sections of dentin

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Abstract

Microradiograms of thin ground sections of transversely cut dentinal tubules were compared to the light microscopic images of the ground sections. The bright rings seen around tubules viewed by ordinary transmitted light with the objective focused on the upper surface of the section could not be related to a radiographically demonstrable peritobular zone. These rings could be produced in rat incisor dentin which does not have peritubular matrix. It was concluded that ground sections viewed with ordinarily transmitted light could not be validly used to determine the presence of peritubular matrix.

Résumé

Des microradiographies de coupes minces minéralogiques de canalicules dentinaires, en coupe transversale, sont comparées a vec les aspects de ces mêmes canalicules en microscopie optique. Les anneaux clairs, visibles autour des canalicules en microscopie à lumière transparentc, en effectuant la mise au point sur la partie supérieure de la coupe, ne peuvent être mis en parallèle avec la zone péricanaliculaire — ces anneaux sont visibles dans la dentine d'incisive de rat, qui ne contient pas de matrice péricanaliculaire. Il semble donc que des coupes par usure, observée en lumière transparente classique, ne permettent pas d'identifier la zone péricanaliculaire.

Zusammenfassung

Röntgenmikrographien von quergetroffenen Zahnbeinkanälchen in Schliffschnitten wurden mit gewöhnlichen lichtmikroskopischen Bildern verglichen. Die hellen Ringe, welche im gewöhnlichen lichtmikroskopischen Bild erscheinen, wenn das Mikroskop über die obere Schnittfläche eingestellt wird, konnten nicht mit einer röntgenmikrographisch dargestellten peritubulären Zone verglichen werden. Diese Ringe konnten im Zahnbein von Rattenschneidezähnen, die keine peritubuläre Zone besitzen, dargestellt werden. Es wurde festgestellt, daß die im gewöhnlichen Licht beobachteten Schliffschnitte nicht für die Herstellung der peritubulären Zonen geeignet sind.

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Weber, D.F. Optical and radiographic appearance of thin ground sections of dentin. Calc. Tis Res. 1, 319–323 (1967). https://doi.org/10.1007/BF02008103

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