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A microspectrophotometric analysis of the effect of fluoride on immature enamel matrix protein of rat molar teeth

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Summary

Over a 24-h period, Wistar rats from 4 litters, 6 to 9 days old, were given five intraperitoneal injections of a solution of 0.9% sodium chloride containing sodium fluoride (3 mg F/kg body weight). Within-litter controls were used. All rats were killed by decapitation 2 h after the final injection and the rat heads, cut sagittally, were processed for protein histochemistry.

The intensity of staining of the protein in the enamel matrix of the upper jaw molar tooth buds was quantified using the two-wavelength method of microphotometry. A significant increase in the intensity of staining of fluoride-treated tissues over controls was observed with the histochemical methods specific for arginine (P<0.01), tyrosine (P < 0.05), and cysteine (P<0.05). Other histochemical methods specific for amino acid groups failed to show any significant difference between fluoride and non-fluoride-treated enamel matrix.

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References

  1. Slavkin, H.C., Trump, G.N., Schonfeld, S.E., Brownell, A., Sorgente, N., Lee-Own, V.: Epigenetic regulation of enamel protein synthesis during epithelial-mesenchymal interactions. In M. Karkinen-Jääskeläinen, L. Saxen, L. Weiss (eds.): Cell Interactions in Differentiation, pp. 209–226. Academic Press, New York, 1977

    Google Scholar 

  2. Deakins, M.: Changes in the ash, water, and organic content of pig enamel during calcification, J. Dent. Res.21:429–435, 1942

    CAS  Google Scholar 

  3. Reith, E.J., Cotty, V.F.: The absorptive activity of ameloblasts during the maturation of enamel, Anat. Rec.157:577–588, 1967

    Article  CAS  PubMed  Google Scholar 

  4. Angmar-Månsson, B.: A quantitative microradiographic study on the organic matrix of developing human enamel in relation to mineral content, Arch. Oral Biol.16:135–145, 1971

    Article  PubMed  Google Scholar 

  5. Glimcher, M.J., Brickley-Parsons, D., Levine, P.T.: Studies of enamel proteins during maturation, Calcif. Tissue Res.24:259–270, 1977

    Article  CAS  PubMed  Google Scholar 

  6. Robinson, C. Fuchs, P., Deutsch, D., Weatherell, J.A.: Four chemically distinct stages in developing enamel from bovine incisor teeth, Caries Res.12:1–11, 1978

    Article  CAS  PubMed  Google Scholar 

  7. Suga, S., Gustafson, G.: Studies on the development of rat enamel by means of histochemistry, microradiography and polarized light microscopy, Adv. Fluor. Res. Dent. Caries Prev.1:233–244, 1963

    Google Scholar 

  8. Matthiessen, M.E.: Nucleic acid and protein histochemical studies on the prenatal development of human deciduous teeth, Acta Anat. (Basel)60:220–238, 1965

    CAS  Google Scholar 

  9. Deguchi, Y.: Histochemical observations of protein-bound amino groups in human developing deciduous teeth, Histochemie7:357–369, 1966

    Article  CAS  PubMed  Google Scholar 

  10. Zerlotti, E., Yaeger, J.A.: Histochemistry and biophysical histology of the matrices of some mineralized tissues, Clin. Orthop. Rel. Res.51:223–254, 1967

    CAS  Google Scholar 

  11. Everett, M.M., Miller, W.A.: Histochemical studies on calcified tissues. 1. Amino acid histochemistry of foetal calf and human enamel matrix, Calcif. Tissue Res.14:229–244, 1974

    Article  CAS  PubMed  Google Scholar 

  12. Fejerskov, O., Thylstrup, A., Joost Larsen, M.: Clinical and structural features and possible pathogenic mechanisms of dental fluorosis, Scand. J. Dent. Res.85:510–534, 1977

    CAS  PubMed  Google Scholar 

  13. Sundström, B., Myhrberg, H.: Light and scanning electron microscopy of fluorosed enamel from human permanent teeth, Caries Res.12:320–328, 1978

    PubMed  Google Scholar 

  14. Newesely, H.: Mechanisms and actions of trace elements in the mineralization of dental hard tissues, pp. 80–86. Zyma S. A. Nyon, Switzerland, 1972

    Google Scholar 

  15. Shinoda, H., Ogura, H.: Scanning electron microscopical study on the fluorosis of enamel in rats, Calcif. Tissue Res.25:75–83, 1978

    Article  CAS  PubMed  Google Scholar 

  16. Thylstrup, A., Fejerskov, O.: The relationship between fluorotic lesions and enamel proteins in human teeth from a high fluoride area, Caries Res.12:121–122, 1978 (abst.)

    Google Scholar 

  17. Kruger, B.J.: The effect of different levels of fluoride on the ultrastructure of ameloblasts in the rat, Arch. Oral Biol.15:109–114, 1970

    Article  CAS  PubMed  Google Scholar 

  18. Kerley, M.A., Kollar, E.J.: Regeneration of tooth developmentin vitro following sodium fluoride treatment, Am. J. Anat.149:181–195, 1977

    Article  CAS  PubMed  Google Scholar 

  19. Kuhar, K.J., Eisenman, D.R.: Fluoride-induced mineralization within vacuoles in maturative ameloblasts of the rat, Anat. Rec.191:91–102, 1978

    Article  CAS  PubMed  Google Scholar 

  20. Kruger, B.J.: An autoradiographic assessment of the effect of fluoride on the uptake of3H-proline by amelobalsts in the rat, Arch. Oral Biol.15:103–108, 1970

    Article  CAS  PubMed  Google Scholar 

  21. Kruger, B.J.: Utilization of3H-serine by ameloblasts of rats receiving sub-mottling doses of fluoride, Arch. Oral Biol.17:1389–1394, 1972

    Article  CAS  PubMed  Google Scholar 

  22. Patterson, C.M., Basford, K.E., Kruger, B.J.: The effect of fluoride on the immature enamel matrix protein of the rat, Arch. Oral Biol.21:131–132, 1976

    Article  CAS  PubMed  Google Scholar 

  23. Smalley, J.W., Embery, G.: Effect of fluoride on molecular size of proteoglycans in the rat incisor tooth, Arch. Oral Biol.21:703–704, 1976

    Article  CAS  PubMed  Google Scholar 

  24. Lillie, R.D., Fullmer, H.M.: Histopathologic Technic and Practical Histochemistry, 4th Ed., pp. 791–794. McGraw-Hill, New York, 1976

    Google Scholar 

  25. Zerlotti, E., Engel, M.B.: The reactivity of proteins of some connective tissues and epithelial structure with 2,4-dinitroluorobenzene, J. Histochem. Cytochem.10:537–546, 1962

    CAS  Google Scholar 

  26. Tranzer, J.-P. Pearse, A.G.E.: Titanous chloride as a reducing agent in the dinitrofluorobenzene reaction for protein, J. Histochem. Cytochem.12:325–326, 1964

    CAS  PubMed  Google Scholar 

  27. Barrnett, R.J., Seligman, A.M.: Histochemical demonstration of protein-bound sulfhydryl groups, Science116:323–327, 1952

    CAS  PubMed  Google Scholar 

  28. Baker, J.R.: The histochemical demonstration of phenols, especially tyrosine, Q. J. Microsc. Sci.97:161–164, 1956

    Google Scholar 

  29. Glenner, G.G., Lillie, R.D.: Observations on the diazo-coupling reaction for the histochemical demonstration of tyrosine, metal chelation and formazan variants, J. Histochem. Cytochem.7:416–422, 1959

    CAS  PubMed  Google Scholar 

  30. Adams, C.W.M.: A p-dimethylaminobenzaldehyde-nitrate method for the histochemical demonstration of tryptophan and related compounds, J. Clin. Pathol.10:56–62, 1957

    CAS  PubMed  Google Scholar 

  31. Lillie, R.D., Pizzolato, P., Dessaur, H.C., Donaldson, P.T.: Histochemical reactions at tissue arginine sites with alkaline solutions ofβ-naphthoquinone-4-sodium sulphonate and other o-quinones and oxidized o-diphenols, a possible mechanism of the Sakaguchi reaction, J. Histochem. Cytochem.19:487–497, 1971

    CAS  PubMed  Google Scholar 

  32. Lillie, R.D., Donaldson, P.T.: Histochemical azo coupling of protein histidine, Brunswik's nitration method, J. Histochem. Cytochem.20:929–937, 1972

    CAS  PubMed  Google Scholar 

  33. Pearse, A.G.E.: Histochemistry, Theoretical and Applied, Vol. 1, 3rd Ed., pp. 171, 612, 620. Churchill, London, 1968

    Google Scholar 

  34. Yaeger, J.A.: Enamel. In S.N. Bhaskar (ed.): Orban's Oral Histology and Embryology, 8th Ed., pp. 45–104. C.V. Mosby, St. Louis, 1976

    Google Scholar 

  35. Ornstein, L.: The distribution error in microspectrophotometry, Lab. Invest.1:250–265, 1952

    CAS  PubMed  Google Scholar 

  36. Patau, K.: Absorption microphotometry of irregular-shaped objects, Chromosoma5:341–362, 1952

    Article  CAS  PubMed  Google Scholar 

  37. Pollister, A.W., Swift, H., Rasch, E.: Microphotometry with visible light. In A.W. Pollister (ed.): Physical Techniques in Biological Research, 2nd Ed., Vol. III, Part C: Cells and Tissues, pp. 201–251. Academic Press, New York, 1969

    Google Scholar 

  38. Schour, I., Massler, M.: The teeth. In E.J. Farris, J.Q. Griffith (eds.): The Rat in Laboratory Investigation, 2nd Ed., pp. 104–165. J.B. Lippincott, Philadelphia, 1949

    Google Scholar 

  39. Sakaguchi, S.: Über eine neue farbenreaktion von protein und arginin, J. Biochem. (Tokyo)5:25–31, 1925

    CAS  Google Scholar 

  40. Kruger, B.J.: Interaction of fluoride and molybdenum on dental morphology in the rat, J. Dent. Res.45:714–725, 1966

    Google Scholar 

  41. Gray, H.S.: A morphological study of the influence of fluoride on rat molar teeth, Arch. Oral Biol.18:1451–1460, 1973

    Article  CAS  PubMed  Google Scholar 

  42. Van Oostveldt, P., Boeken, G.: Two-wavelength cytophotometry, the choice of the wavelengths from a practical point of view, J. Histochem. Cytochem.25:1337–1344, 1977

    PubMed  Google Scholar 

  43. Rasch, E., Swift, H.: Microphotometric analysis of the cytochemical Millon reaction, J. Histochem. Cytochem.8:4–17, 1960

    CAS  PubMed  Google Scholar 

  44. Ritter, C., Berman, J.: The quantitative cytophotometric analysis of tyrosine by a modified diazotization-coupling method, J. Histochem. Cytochem.11:590–602, 1963

    CAS  Google Scholar 

  45. Bloch, D.P.: Cytochemistry of the histones, ProtoplasmatologiaV3d:1–56, 1966

    Google Scholar 

  46. Augsten, K., Hesse, G., Zschiesche, W.: Quantitative mikrospektrophotometrische untersuchungen zum tryptophannachweis nach Adams, Histochemie19:44–57, 1969

    Article  CAS  PubMed  Google Scholar 

  47. Esterbauer, H., Nöhammer, G., Schauenstein, E., Weber, P.: Beitrag zum quantitative histochemischen Nachweis von Sulfhydrylgruppen mit der DDD-Färbung, III. Quantitative cytospekrometrische Bestimmungen, an Ehrlich-Ascites-Tumorzellen, Acta Histochem. (Jena)47:106–144, 1973

    CAS  Google Scholar 

  48. McLeish, J., Bell, L.G.E., LaCour, L.F., Chayen, J.: The quantitative cytochemical estimation of arginine, Exp. Cell Res.12:120–125, 1957

    Article  CAS  PubMed  Google Scholar 

  49. Rasch, E., Woodard, J.W.: Basic proteins of plant nuclei during normal and pathological cell growth, J. Biophys. Biochem. Cytol.6:263–276, 1959

    Article  CAS  PubMed  Google Scholar 

  50. Deitch, A.D.: An improved Sakaguchi reaction for microspectrometric use, J. Histochem. Cytochem.9:477–483, 1961

    CAS  PubMed  Google Scholar 

  51. Wennberg, A., Bawden, J.W.: Comparison of33P and45Ca distribution in developing rat molar enamel in vivo and in vitro, J. Dent. Res.57:111–117, 1978

    CAS  PubMed  Google Scholar 

  52. Avery, J.K., Visser, R.L., Knapp D.E.: The pattern of the mineralization of enamel, J. Dent. Res.40:1004–1019, 1961

    Google Scholar 

  53. Crabb, H.S.M., Darling, A.I.: The pattern of progressive mineralisation in human dental enamel, Internat. Ser. Monographs Oral Biol., Vol. 2. Pergamon, Oxford, 1962

    Google Scholar 

  54. Rosser, H., Boyde, A., Stewart, A.D.G.: Preliminary observations of the calcium concentration in developing enamel assessed by scanning electron-probe x-ray emission microanalysis, Arch. Oral Biol.12:431–440, 1967

    Article  CAS  PubMed  Google Scholar 

  55. Cooper, W.E.G.: A microchemical, microradiographic and histological investigation of amelogenesis in the pig, Arch. Oral Biol.13:27–48, 1968

    Article  CAS  PubMed  Google Scholar 

  56. Suga, S., Murayama, Y., Musashi, T.: A study of the mineralization process in the developing enamel of guinea pigs, Arch. Oral Biol.15:597–612, 1970

    Article  CAS  PubMed  Google Scholar 

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  1. Department of Oral Biology, Physiology Building, University of Queensland, St. Lucia, 4067, Brisbane, Queensland

    J. R. Smid & B. J. Kruger

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Smid, J.R., Kruger, B.J. A microspectrophotometric analysis of the effect of fluoride on immature enamel matrix protein of rat molar teeth. Calcif Tissue Int 30, 57–66 (1980). https://doi.org/10.1007/BF02408607

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