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Biochemical characterization of 1,25(OH)2D3 receptors in chick embryonal duodenal cytosol

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Summary

This study presents measurements of serum vitamin D metabolites, calcium and phosphorus as well as measurements of the equilibrium dissociation constant for duodenal 1,25(OH)2D3 receptor in 15-, 18-, 19-, and 20-day chick embryos in comparison to that in 1- and 118-day-old chicks and to vitamin D-deficient chicks. The present results showed that: (a) serum 1,25(OH)2D and 24,25(OH)2D levels rise from 15 and 18 to days 19 and 20 of embryonic development while serum phosphate levels are stable; (b) serum calcium levels rise at hatching to adult levels; (c) the duodenal 1,25(OH)2D3 receptor is detectable in 15-day-old embryo and has a Kd similar to that of 118-day-old vitamin D-replete chicks; and (d) the activity of 1,25(OH)2D3 receptor in chick duodenal cytosol is maximal at hatching.

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Seino, Y., Yamaoka, K., Ishida, M. et al. Biochemical characterization of 1,25(OH)2D3 receptors in chick embryonal duodenal cytosol. Calcif Tissue Int 34, 265–269 (1982). https://doi.org/10.1007/BF02411248

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