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Influence of vitamin A on matrix-induced endochondral bone formation

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Summary

The influence of retinoic acid on matrix-induced endochondral bone differentiation was determined. Retinoic acid was administered during discrete stages of endochondral bone formation, specifically, mesenchymal cell proliferation, chondrogenesis, bone formation, and mineralization. In retinoic acid-treated rats examined on day 3 following matrix implantation, biochemical markers for mesenchymal cell proliferation were about 50% of the controls. Chondrogenesis on day 7, assessed by35SO4 incorporation into proteoglycans, was 27% of the control. In addition, dissociative extraction of proteoglycans with 4.0 M guanidine-HCl and chromatography on Sepharose CL-2B revealed the synthesis of a smaller molecular weight proteoglycan when compared to controls which exhibited the cartilage-specific type. Osteogenesis and bone mineralization were monitored by alkaline phosphatase activity and45Ca incorporation. On day 11 alkaline phosphatase activity was decreased by 40% and45Ca incorporation was 48% of the control. These results revealed the multiple foci of the actions of excess vitamin A.

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Authors and Affiliations

  1. Laboratory of Biological Structure, National Institute of Dental Research, National Institutes of Health, 20205, Bethesda, Maryland, USA

    D. P. DeSimone & A. H. Reddi

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  1. D. P. DeSimone
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  2. A. H. Reddi
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DeSimone, D.P., Reddi, A.H. Influence of vitamin A on matrix-induced endochondral bone formation. Calcif Tissue Int 35, 732–739 (1983). https://doi.org/10.1007/BF02405115

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