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Effect of dehydroepiandrosterone sulfate on the mineral accretion of chick embryo frontal bones cultivatedin vitro

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Abstract

Chick embryo frontal bones at 12 and 13 days of development cultivatedin vitro exhibit different patterns of glucose utilization, periosteal cellular density and calcium and hydroxyproline content. When dehydroepiandrosterone sulfate is added to the medium at a concentration 1 mM, 12-day frontals engage primarily in osteoid tissue synthesis while 13-day frontals calcify at a significantly greater rate than controls.

Measured with the ratio calcium/hydroxyproline, the calcification of 13-day frontals follows a linear function with the logarithm of the doses of dehydroepiandrosterone sulfate employed (0.5, 1.0 and 2.0 mM).

The 13-day frontal bone cultivatedin vitro seems to be an adequate experimental model for the study of the effects of dehydroepiandrosterone sulfate on bone tissue because it reproduces the basic phenomenon (increased calcification) observed in man and animals treated with androgens.

Résumé

Les modes d'utilisation de glucose, le contenu de calcium et d'hydroxyproline et la densité cellulaire du perioste de les os frontaux d'embryons de poulet de 12 et 13 jours de developpement, cultivés sur coagulum de plasma, se presentant différenment à chaque âge. Cultivés avec sulfate de déhydroèpiandrostérone en concentration 1 mM, les frontaux de 12 jours montrent un synthese augmentée du matrice osseuse, celle de 13 jours se calcifient à une vélocité significativement plus grande que celle des os contôles.

Le degré de calcification au quatrième jour de culture measuré par la relation calcium/hydroxyproline, suit un fonction lineáire avec le logarithme des doses de sulfate de dehydroepiandrostérone employées (0.5, 1,0 et 2,0 mM).

Les renseignements obtenus indiquent que les frontaux de 13 jours, cultivés “in vitro” constituent modeles experimentaux appropriés pour étudier l'effet des androgénes sur le tissue osseux.

Zusammenfassung

Stirnbeine von Hühnerembryonen an ihrem 12. und 13. Entwicklungstag entnommen und in vitro kultiviert zeigen verschiedene Arten der Glucoseverwertung der Periostzellendichte, des Calcium- und Hydroxyprolingehaltes. Wird Dehydroepiandrosteronsulfat dem Medium in einer 1 mM-Konzentration zugegeben, so beteiligen sich die 12tägigen Stirnbeine vorwiegend an der Knochengewebesynthese, während die 13tägigen signifikant stärker verkalken als die Kontrollen.

Gemessen an der Calcium/hydroxyprolin Ratio bildet die Verkalkung der 13tägigen Stirnbeine eine lineare Funktion mit den Logarithmen der verwendeten Dosen von Dehydroepiandrosteronsulfat (0,5, 1,0 und 2,0 mM).

Das in vitro kultivierte 13tägige Stirnbein schein ein geeignetes Experimentiermodell zur Studie der Dehydroepiandrosteronsulfatwirkung auf das Knochengewebe zu sein, weil es das grundlegende Phänomen (erhöhte Verkalkung) wiedergibt, welches man auch bei mit Androgenen behandelten Menschen und Tieren beobachtet.

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Puche, R.C., Romano, M.C. Effect of dehydroepiandrosterone sulfate on the mineral accretion of chick embryo frontal bones cultivatedin vitro . Calc. Tis Res. 4, 39–47 (1969). https://doi.org/10.1007/BF02279104

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  • DOI: https://doi.org/10.1007/BF02279104

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