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The proto-oncogene C-raf-1 is highly expressed only in the hypertrophic zone of the growth plate

  • Molecular and Cellular Biology
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Abstract

Proto-oncogene c-raf-1, the cellular homologue of the acutely transforming oncogene v-raf, has a central role in the signal tranduction pathways. The growth plate, due to its non-overlapping zones of chondrocyte maturation, provides a physiological in situ model for investigating the role of c-raf-1 in proliferation and differentiation of chondrocytes. In this study, Northern blotting was first performed to examine the expression of mRNA for c-raf-1 in the embryonic chick tibial growth plate. It revealed that the normal levels of c-raf-1 mRNA were associated with the whole growth plate. We then investigated the localization of c-raf-1 mRNA and c-raf-1 protein in the growth plate by in situ hybridization and immunohistochemistry in order to determine whether c-raf-1 is involved in chondrocyte maturation. Our results showed that c-raf-1 mRNA and c-raf-1 protein were detected only in the hypertrophic zone. The data suggest involvement of this proto-oncogene in chondrocyte differentiation and/or hypertrophy rather than in proliferation.

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Authors and Affiliations

  1. Department of Oral Surgery, School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, 260, Chiba, Japan

    Y. Kaneko, H. Tanzawa & K. Sato

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  1. Y. Kaneko
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  2. H. Tanzawa
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  3. K. Sato
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Kaneko, Y., Tanzawa, H. & Sato, K. The proto-oncogene C-raf-1 is highly expressed only in the hypertrophic zone of the growth plate. Calcif Tissue Int 54, 426–430 (1994). https://doi.org/10.1007/BF00305531

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  • DOI: https://doi.org/10.1007/BF00305531

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