Abstract
A method of fixation has been developed for the cartilage cells of the epiphyseal plate. Improved preservation of the flattened chondrocytes reveals small vesicles that seem to originate in the rough endoplasmic reticulum and are found in large amounts in the Golgi area. Different types of vacuoles can be distinguished in the Golgi complex, e.g. “intermediate vacuoles” and “large vacuoles”, the latter usually containing inclusions. It seems likely that the vesicles and vacuoles each play a specific role in the transport and production of sulfated protein polysaccharides.
Résumé
Une méthode de fixation des cellules cartilagineuses de la métaphyse est mise au point. Une meilleure préservation des chondrocytes applatis montre de petites vésicules qui semblent naître dans l'ergastoplasme et se trouvent en grande quantité au niveau de l'appareil de Golgi. Différents types de vacuoles sont visibles dans cet appareil, à savoir des ≪vacuoles intermédiaires≫ et de ≪larges vacuoles≫ contenant des inclusions. Il parait probable que les vésicules et vacuoles jouent un rôle spécifique dans le transport et la production de polysaccharides de protéines sulfatées.
Zusammenfassung
Es wurde eine Methode zur Fixation der Knorpelzellen aus der Epiphysenplatte entwickelt. die besser erhaltenen abgeflachten Chondrocyten zeigen kleine Bläschen, welche aus dem groben endoplasmatischen Reticulum zu stammen scheinen und in großen Mengen im Golgi-Bereich vorliegen. Verschiedene Arten von Vacuolen können im Golgi-Apparat unterschieden werden, z.B. “intermediate vacuoles” und “large vacuoles”, wobei die letztgenannten gewöhnlich Einschlüsse enthalten. Es ist wahrscheinlich, daß die Bläschen sowie die Vacuolen eine spezifische Rolle beim Transport und der Bildung von sulfatierten Proteinpolysacchariden spielen.
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Holtrop, M.E. The ultrastructure of the epiphyseal plate. Calc. Tis Res. 9, 131–139 (1972). https://doi.org/10.1007/BF02061951
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DOI: https://doi.org/10.1007/BF02061951