Summary
Stationary electrical conductance experiments together with nonstationary relaxation experiments allow a quantitative determination of rate constants describing carrier-mediated ion transport. Valinomycin-induced ion transport across neutral lipid membranes was studied. The dependence of the transport parameters on the chain length of the lipid molecules, on the kind of alkali ion, and on the temperature was determined. The relaxation time τ the current following a voltage jump shows a marked increase with decreasing temperature or with increasing chain length of the lipid molecules. This variation of τ is interpreted on the basis of a varying membrane fluidity. It is shown that under favorable circumstances the equilibrium constant of complex formation in the aqueous phase may be obtained from membrane experiments. Furthermore, the kinetics of exchange of valinomycin between membrane and water was studied. We found a marked influence of the totus surrounding the black film on the kinetics as well as on the total amount of valinomycin molecules in the membrane. The problem of location of the free carrier molecules inside the membrane is discussed.
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Andreoli, T. E., Tieffenberg, M., Tosteson, D. C. 1967. The effect of valinomycin on the ionic permeability of thin lipid membranes.J. Gen. Physiol. 50:2527
Baer, F., Buchnea, D. 1959. Synthesis of saturated and unsaturatedl-α-lecithins.Canad. J. Biochem. Physiol. 37:953
Bounds, D. G., Linstead, R. P., Weedon, B. C. L. 1954. Anodic syntheses. Part X. Synthesis of nervonic (selacholeic) acid.J. Chem. Soc. 1954:448
Carlslaw, H. S., Jaeger, J. C. 1959. Conduction of Heat in Solids. p. 101. Clarendon Press, Oxford
Ciani, S., Eisenman, G., Szabo, G. 1969. A theory for the effects of neutral carriers such as the macrotetralide actin antibiotics on the electrical properties of bilayer membranes.J. Membrane Biol. 1:1
Ciani, S., Laprade, R., Eisenman, G., Szabo, G. 1973. Theory for carrier-mediated zerocurrent conductance of bilayers extended to allow for nonequilibrium of interfacial reactions, spatially dependent mobilities and barrier shape.J. Membrane Biol. 11:255
Eisenman, G., Ciani, S. M., Szabo, G. 1968. Some theoretically expected and experimentally observed properties of lipid bilayer membranes containing neutral molecular carriers of ions.Fed. Proc. 27:1289
Eisenman, G., Szabo, G., McLaughlin, S. G. A., Ciani, S. M. 1972. Molecular basis for the action of macrocyclic antibiotics on membranes.In: Proceedings of the Symposium on Molecular Mechanisms of Antibiotic Action on Protein Biosynthesis and Membranes. E. Munoz, F. Garcia Ferrandiz and D. Vazquez, editors. p. 545. Elsevier Publishing Co., Amsterdam
Fettiplace R., Andrews, D. M., Haydon, D. A. 1971. The thickness, composition and structure of some lipid bilayers and natural membranes.J. Membrane Biol. 5:277
Grell, E., Funck, T., Eggers, F. 1972. Dynamic properties and membrane activity of ion specific antibiotics.In: Proceedings of the Symposium on Molecular Mechanism of Antibiotic Action on Protein Biosynthesis and Membranes. E. Munoz, F. Garcia Ferrandiz and D. Vazquez, editors. p. 646. Elsevier Publishing Co., Amsterdam
Hall, J. E., Mead, C. A., Szabo, G. 1973. A barrier model for current flow in lipid bilayer membranes.J. Membrane Biol. 11:75
Haydon, D. A., Hladky, S. B. 1972. Ion transport across thin lipid membranes: A critical discussion of mechanisms in selected systems.Quart. Rev. Biophys. 5:187
Hladky, S. B. 1972. The steady-state theory of the carrier transport of ions.J. Membrane Biol. 10:67
Hladky, S. B. 1973. The effect of stirring on the flux of carriers into black lipid membranes.Biochim. Biophys. Acta 307:261
Ketterer, B., Neumcke, B., Läuger, P. 1971. Transport mechanism of hydrophobic ions through lipid bilayer membranes.J. Membrane Biol. 5:225
Krasne, S., Eisenman, G., Szabo G. 1971. Freezing and melting of bilayers and the mode of action of nonactin, valinomycin and gramicidin.Science 174:412
Läuger, P., Stark, G. 1970. Kinetics of carrier-mediated ion transport across lipid bilayer membranes.Biochim. Biophys. Acta 211:458
Lev, A. A., Buzhinsky, E. P. 1967. Cation specificity of the model bimolecular phospholipid membranes with incorporated valinomycin.Tsitologiya 9:102
Liberman, E. A., Topaly, V. P. 1968. Selective transport of ions through bimolecular phospholipid membranes.Biochim. Biophys. Acta 163:125
Markin, V. S., Kristalik, L. I., Liberman, E. A., Topaly, V. P. 1969. Mechanism of conductivity of artificial phospholipid membranes in presence of ion carriers.Biofizika 14:256
Mueller, P., Rudin, D. O. 1967. Development of K+−Na+ discrimination in experimental bimolecular lipid membranes by macrocyclic antibiotics.Biochem. Biophys. Res. Commun. 26:398
Neumcke, B., Läuger, P. 1969. Nonlinear electrical effects in lipid bilayer membranes. II. Integration of the generalized Nernst-Planck equation.Biophys. J. 9:1160
Pressman, B. C. 1968. Ionophorous antibiotics as models for biological transport.Fed. Proc. 27:1283
Shemyakin, M. M., Ovchinnikov, Yu. A., Ivanov, V. T., Antonov, V. K., Vinogradova, E. I., Shkrob, A. M., Malenkov, G. G., Evstratov, A. V., Laine, I. A., Melnik, E. I., Ryabova, I. D. 1969. Cyclodepsipeptides as chemical tools for studying ionic transport through membranes.J. Membrane Biol. 1:402
Stark, G. 1973. Rectification phenomena in carrier mediated ion transport.Biochim. Biophys. Acta 298:323
Stark, G., Benz, R. 1971. The transport of potassium through lipid bilayer membranes by the neutral carriers valinomycin and monactin.J. Membrane Biol. 5:133
Stark, G., Benz, R., Pohl, G., Janko, K. 1972. Valinomycin as a probe for the study of structural changes of black lipid membranes.Biochim. Biophys. Acta 266:603
Stark, G., Ketterer, B., Benz, R., Läuger, P. 1971. The rate constants of valinomycin-mediated ion transport through thin lipid membranes.Biophys. J. 11:981
Szabo, G., Eisenman, G., Ciani, S. 1969. The effects of the macrotetralide actin antibiotics on the electrical properties of phospholipid bilayer membranes.J. Membrane Biol. 1:346
White, S. H. 1970. A study of lipid bilayer membrane stability using precise measurements of specific capacitance.Biophys. J. 10:1127
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Benz, R., Stark, G., Janko, K. et al. Valinomycin-mediated ion transport through neutral lipid membranes: Influence of hydrocarbon chain length and temperature. J. Membrain Biol. 14, 339–364 (1973). https://doi.org/10.1007/BF01868084
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DOI: https://doi.org/10.1007/BF01868084