Summary
Energy expenditure and transepithelial sodium transport were measured continuously and simultaneously from isolated urinary bladders of the Dominican toad,Bufo marinus. Sodium transport was measured as the short-circuit current and CO2 produced by the bladder was measured conductometrically by the method of Maffly. The rates of sodium transport and CO2 production were linearly related. The slope of the regression of sodium transport on CO2 production,dJ Na/dJ CO 2, was found to be quite similar in paired half bladders but to differ significantly between bladders from different toads. Thus, in this preparation there appears to be no unique stoichiometric ratio characterizing sodium transport and metabolism and past efforts to arrive at such a value by averaging results obtained from different animals do not seem warranted. The CO2 production by the isolated bladder which is unrelated to sodium transport was determined by two means: 1) extrapolating the regression ofJ Na onJ CO 2 toJ Na=0, and 2) measuring CO2 production with sodium transport suppressed by removal of all sodium from the mucosal bathing medium. The two methods gave values which were in close agreement in each preparation. This suggests that metabolism which supports nontransport activities in this tissue cannot be recruited to support the energy requirement of sodium transport and vice versa.
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References
Civan, M. M. 1970. Effects of active sodium transport on current voltage relationship of toad bladder.Amer. J. Physiol. 219:234
Finn, A. L., Handler, J. S., Orloff, J. 1967. Active chloride transport in the isolated toad bladder.Amer. J. Physiol.,213:179
Frazier, L. W., Vanatta, J. C. 1971. Excretion of H+ and NH +4 by the urinary bladder of the acidotic toad and the effect of short-circuit current on the excretion.Biochim. Biophys. Acta 241:20
Kramer, K., Deetjen, P. 1964. Oxygen consumption and sodium reabsorption in the mammalian kidney.In: Oxygen Consumption in the Animal Organism F. Dickens and E. Neil, editors. p. 411. MacMillan Company, New York
Leaf, A. 1965. Transepithelial transport and its hormonal control in toad bladder.Ergebn. Physiol. 56:215
Leaf, A., Anderson, J., Page, L. 1958. Active sodium transport by the isolated toad bladder,J. Gen. Physiol. 41:657
Leaf, A., Page, L. B., Anderson, J. 1959. Respiration and active sodium transport of isolated toad bladder.J. Biol. Chem. 234:1625
Leaf, A., Renshaw, A. 1957. Ion transport and respiration of isolated frog skin.Biochem. J. 65:82
Ludens, J. H., Fanestil, D. D. 1972. Acidification of urine by the isolated urinary bladder of the toad.Amer. J. Physiol. 223:1338
Maffly, R. H., Steele, R. E., Walker, W. E., Coplon, N. S. 1973. Respiration and active sodium transport.Abstr. Amer. Soc. Nephrol. p. 72
Martin, D. W., Diamond, J. M., 1966. Energetics of coupled active transport of sodium and chloride.J. Gen. Physiol. 50:295
Nellans, H. N., Finn, A. L. 1974. Oxygen consumption and sodium transport in the toad urinary bladder.Amer. J. Physiol. 227:670
Rosen, S., Oliver, J. A., Steinmetz, P. R. 1974. Urinary acidification and carbonic anhydrase distribution in bladders of Dominican and Colombian toads.J. Membrane Biol. 15:193
Scholander, P. F. 1942. Volumetric microrespirometers.Rev. Sci. Instr. 13:32
Snedecor, G. W., Cochran, W. G. 1967. Statistical Methods, 6th Edition. Iowa State University Press, Ames, Iowa
Vieira, F. L., Caplan, S. R., Essig, A. 1972. Energetics of sodium transport in frog skin. I. Oxygen consumption in the short-circuited state.J. Gen. Physiol. 59:60
Zerahn, K. 1956. Oxygen consumption and active sodium transport in the isolated and short-circuited frog skin.Acta Physiol. Scand. 36:300
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Al-Awqati, Q., Beauwens, R. & Leaf, A. Coupling of sodium transport to respiration in the toad bladder. J. Membrain Biol. 22, 91–105 (1975). https://doi.org/10.1007/BF01868165
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DOI: https://doi.org/10.1007/BF01868165