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Passive sodium fluxes across toad bladder in the presence of simultaneous transepithelial gradients of concentration and potential

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Summary

Bidirectional sodium fluxes were measured across toad bladder sacs after eliminating active transport with ouabain. Transepithelial potential was clamped to 100 mV or the Nernst potential,ψ eq, at varying sodium concentrations,C m , in the mucosal medium. Serosal sodium concentration,C s , was held constant. Equations were derived for permeability, partial ionic conductance, and unidirectional fluxes as functions ofC m andC s , based in part on the assumption that the ratio,Q, of bulk sodium permeability to tracer sodium permeability is a constant, independent of concentration and potential. The results conformed closely to these equations.

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References

  1. Bindslev, N., Tormey, J. M., Pietras, R. J., Wright, E. A. 1974. Electrically and osmotically induced changes in permeability and structure of toad urinary bladder.Biochim. Biophys. Acta 332:286.

    Google Scholar 

  2. Chen, J. S., Walser, M. 1974. Passive ion fluxes across toad bladder.J. Membrane Biol. 18:365

    Article  Google Scholar 

  3. Chen, J. S., Walser, M. 1975. Sodium fluxes through the active transport pathway in toad bladder.J. Membrane Biol. 21:87

    Google Scholar 

  4. Chen, J. S., Walser, M. 1976. Effect of transepithelial concentration gradients on the passive fluxes of sodium across toad bladder.J. Membrane Biol. 27:381.

    Article  Google Scholar 

  5. Kedem, O., Essig, A. 1965. Isotope flows and flux ratios in biological membranes.J. Gen. Physiol. 48:1047.

    PubMed  Google Scholar 

  6. Krämer, H., Meares, P. 1969. Correlation of electrical and permeability properties of ion-selective membranes.Biophys. J. 9:1006

    PubMed  Google Scholar 

  7. Kotyk, A., Janacek, K. 1970. Cell Membrane Transport. p. 50. Plenum Press, New York

    Google Scholar 

  8. Lindemann, B., Grebhart, U. 1973. Delayed change of Na-permeability in response to steps of (Na) at the outer surface of frog skin and frog bladder.In: Transport Mechanisms in Epithelia. H. H. Ussing and N. A. Thorn, editors. p. 115. Munksgaard, Copenhagen

    Google Scholar 

  9. Mandel, L. J., Curran, P. F. 1972. Response of the frog skin to steady-state voltage clamping. I. The shunt pathway.J. Gen. Physiol. 59:503

    PubMed  Google Scholar 

  10. Reuss, L., Finn, A. L. 1975. Effects of changes in the composition of the mucosal solution on the electrical properties of the toad urinary bladder epithelium.J. Membrane Biol. 20:191

    Article  Google Scholar 

  11. Walser, M. 1972. Components of sodium and chloride flxx across toad bladder.Biophys. J. 12:351

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology and Experimental Therapeutics and Department of Medicine, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, 21205, Baltimore, Maryland

    Jing S. Chen & Mackenzie Walser

Authors
  1. Jing S. Chen
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  2. Mackenzie Walser
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Chen, J.S., Walser, M. Passive sodium fluxes across toad bladder in the presence of simultaneous transepithelial gradients of concentration and potential. J. Membrain Biol. 32, 319–330 (1977). https://doi.org/10.1007/BF01905225

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  • DOI: https://doi.org/10.1007/BF01905225

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