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Proteolytic degradation of human erythrocyte band 3 by membrane-associated protease activity

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Summary

Antisera directed against the cytoplasmic portion of human erythrocyte Band 3 were used to follow the degradation of the band 3 molecule. Small amounts of Band 3 were degraded when well-washed red cell membrane ghosts were incubated in the cold; this process was greatly accelerated by incubating ghosts at 37°C. Band 3 labeled with pyridoxal-phosphate was digested at comparable rates. Band 3 digestion also took place when alkali-extracted ghost membranes were incubated at 37° for prolonged periods. These results suggest that human erythrocytes contain tightly bound, membrane-associated proteolytic activity.

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References

  1. Bernacki, R.J., Bosmann, H.B. 1972. Red cell hydrolases. II. Proteinase activities in human erythrocyte plasma membranes.J. Membrane Biol. 7:1

    Google Scholar 

  2. Billah, M.M., Finean, J.B., Coleman, R., Michell, R.H. 1976. Preparation of erythrocyte ghosts by a glycol-induced osmotic lysis under isoionic conditions.Biochim. Biophys. Acta 433:54

    Google Scholar 

  3. Blobel, G., Dobberstein, B. 1975. Transfer of proteins across membranes.J. Cell Biol. 67:835

    Google Scholar 

  4. Bretscher, M.S. 1971. A major protein which spans the human erythrocyte membrane.J. Mol. Biol. 59:351

    Google Scholar 

  5. Burkholder, D.E., Brecher, A.S. 1974. Interaction between proteases and bovine erythrocyte membranes. II. Binding of fibrinolytic components.332:201

  6. Cabantchik, Z., Balshin, M., Breuer, W., Rothstein, A. 1975. Pyridoxal phosphate: An amonic probe for protein amino groups exposed on the outer and inner surfaces of intact human blood cells.J. Biol. Chem. 250:5130

    Google Scholar 

  7. Cotmore, S.F., Furthmayr, H., Marchesi, V.T. 1977. Immunochemical evidence for the transmembrane orientation of glycophorin A localization of ferritin-antibody conjugates in intact cells.J. Mol. Biol. 113:539

    Google Scholar 

  8. Douglas, R.H., Hultquist, D.E. 1978. Evidence that two forms of bovine erythrocyte cytochromeb 5 are identical to segments of microsomal cytochromeb 5.Proc. Nat. Acad. Sci. USA 75:3118

    Google Scholar 

  9. Drickamer, L.K. 1977. Fragmentation of the band 3 polypeptide from human erythrocyte membranes. Identification of regions likely to interact with the lipid bilayer.J. Biol. Chem. 252:6909

    Google Scholar 

  10. Fairbanks, G., Steck, T.L., Wallach, D.F.H. 1971. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.Biochemistry 10:2607

    Google Scholar 

  11. Fukuda, M., Eshdat, Y., Tarone, G., Marchesi, V.T. 1978. Isolation and characterization of peptides derived from the cytoplasmic segment of band 3, the predominant intrinsic membrane protein of the human erythrocyte.J. Biol. Chem. 253:2419

    Google Scholar 

  12. Furthmayr, H., Galardy, R.E., Tomita, M., Marchesi, V.T. 1978. The intramembranous segment of human erythrocyte glycophorin A.Arch. Biochem. Biophys. 185:21

    Google Scholar 

  13. Morrison, W.L., Neurath, H. 1953. Proteolytic enzymes of the formed elements of human blood. I. Erythrocytes.J. Biol. Chem. 200:39

    Google Scholar 

  14. Rothstein, A., Cabantchik, Z.I., Knauf, P. 1976. Mechanism of anion transport in red blood cells: Role of membrane proteins.Fed. Proc. 35:3

    Google Scholar 

  15. Steck, T.L. 1974. The organization of proteins in the human red blood cell membrane.J. Cell Biol. 62:1

    Google Scholar 

  16. Steck, T.L., Koziarz, J.J., Singh, M.K., Reddy, G., Kohler, H. 1978. Preparation and analysis of seven major topographically defined fragments of band 3, the predominant transmembrane polypeptide of human erythrocyte membranes.Biochemistry 17:1216

    Google Scholar 

  17. Steck, T.L., Ramos, B., Strapazon, E. 1976. Proteolytic dissection of band 3, the predominant transmembrane polypeptide of the human erythrocyte membrane.Biochemistry 15:1154

    Google Scholar 

  18. Steck, T.L., Yu, J. 1973. Selective solubilization of proteins from red blood cell membranes by protein perturbants.J. Supramol. Struct. 1:220

    Google Scholar 

  19. Tokes, Z.A., Chambers, S.M. 1975. Proteolytic activity associated with human erythrocyte membranes. Self-digestion of isolated human erythrocyte membranes.Biochim. Biophys. Acta 389:325

    Google Scholar 

  20. Tomita, M., Furthmayr, H., Marchesi, V.T. 1978. Primary structure of human erythrocyte glycophorin A. Isolation and characterization of peptides and complete amino acid sequence.Biochemistry 17:4756

    Google Scholar 

  21. Tomita, M., Marchesi, V.T. 1975. Amino-acid sequence and oligosaccharide attachment sites of human erythrocyte glycophorin.Proc. Nat. Acad. Sci. USA 72:2964

    Google Scholar 

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Author notes

  1. G. Tarone

    Present address: Facolta di Medicina, Cattedra di Istologia ed Embriologia, Universita di Trieste, Trieste, Italy

  2. N. Hamasaki

    Present address: Fukuoka University School of Medicine, Nanakuma Nishi-ku, 814, Fukuoka, Japan

  3. M. Fukuda

    Present address: Division of Biochemical Oncology, Fred Hutchinson Cancer Research Center, University of Washington, Seattle, WA

Authors and Affiliations

  1. Department of Pathology, Yale University School of Medicine, 06510, New Haven, Connecticut

    G. Tarone, N. Hamasaki, M. Fukuda & V. T. Marchesi

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  1. G. Tarone
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  2. N. Hamasaki
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  3. M. Fukuda
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  4. V. T. Marchesi
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Tarone, G., Hamasaki, N., Fukuda, M. et al. Proteolytic degradation of human erythrocyte band 3 by membrane-associated protease activity. J. Membrain Biol. 48, 1–12 (1979). https://doi.org/10.1007/BF01869253

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  • DOI: https://doi.org/10.1007/BF01869253

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