Summary
The effects of hydrostatic pressure and temperature on the three components of K+ uptake in human red cells have been investigated, using ouabain and bumetanide to distinguish between the pump, passive diffusion and cotransport. The pressure sensitivity for passive diffusion has been shown to depend on the counter-ion present. The order of this effect, Cl−>Br−>NO −3 >I−, is the same as for the ionic partial modal volumes and the Hofmeister series. We have analyzed our experimental results thermodynamically, and propose a model for the activated transition-state complex of the potassium ion which involves the loss of water molecules from the secondary hydration shell, cosphere II.
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References
Aveyard, R., Haydon, D.A. 1973. An introduction to the principles of surface chemistry. Cambridge University Press, Cambridge, p. 111
Bockris, J.O'M. 1949. Ionic solvation.Q. Rev. 3:173–180
Catton, W.T. 1957. Physical Methods in Physiology. Pitman, London
Davson, H. 1940. The influence of the lyotropic series of anions on cation permeability.Biochem. J. 34:917–925
Dixon, M., Webb, E.C. 1965. Enzymes. Longmans, London
Dreizen, P., Kim, H.D. 1971. Contractile proteins of a benthic fish. I. Effects of temperature and pressure.Am. Zool. 11:513–521
Dunham, P.B., Ellory, J.C. 1980. Stimulation of the Na/K pump by trypsin in erythrocytes of LK goats.J. Physiol. (London) 301:25–38
Dunham, P.B., Stewart, G.W., Ellory, J.C. 1980. Chloride-activated passive potassium transport in human erythrocytes.Proc. Natl. Acad. Sci. USA 77:1711–1715
Franks, F. 1973. Water. Vol. 3. Plenum Press, New York & London
Friedman, H.L., Krishnan, C.V. 1973. Thermodynamics of ionic hydration.In: Water. F. Franks, editor. Vol. 3, Ch. 1, p. 1. Plenum Press, New York & London
Funder, J., Wieth, J.O. 1967. Effects of some monovalent anions on fluxes of Na+ and K+ and on glucose metabolism of ouabain treated human red cells.Acta Physiol. Scand. 71:168–185
Glynn, I.M. 1956. Sodium and potassium movements in human red cells.J. Physiol. (London) 134:278–310
Glynn, I.M. 1957. The ionic permeability of the red cell membrane.Prog. Biophys. Biophys. Chem. 8:241–307
Goldinger, J.M., Kang, B.S., Choo, Y.E., Paganelli, C.V., Hong, S.K. 1980. Effect of hydrostatic pressure on ion transport and metabolism in human erythrocytes.J. Appl. Physiol. 49:224–231
Hochachka, P.W., Somero, G.N. 1973. The basic effects of pressure on biological systems.In: Strategies of Biochemical Adaptation. Ch. 8, p. 271. P.W. Hochachka and G.N. Somero, editors. W.B. Saunders, Philadelphia
Hofmeister, F. 1888. Zur Lehre von der Wirkung der Salze.Arch. Exp. Pathol. Pharmakol. 24:247–260;also (1889)25:1–30; (1890)27:395–413; (1891)28:210–238
Johnson, F.H., Eyring, H., Stover, B.J. 1974. The Theory of Rate Processes in Biology and Medicine. John Wiley & Sons, New York & London
Kavanau, J.L. 1965. Structure and Function in Biological Membranes. Vol. I, p. 229. Holden-Day Inc., San Francisco
Kim, H.D., Dreizen, P. 1971 Contractile proteins of a benthic fish. II. Composition and ATPase properties of actomyosin.Am. Zool. 11:523–529
Knauf, P., Rothstein, A. 1971. Chemical modification of membranes. Effects of sulphydryl and amino reactive agents on anion and cation permeability of the human red blood cell.J. Gen. Physiol. 58:190–210
Lakshminarayanaiah, N. 1969. Transport Phenomena in Membranes. Ch. 6, p. 300. Academic Press, New York & London
Macdonald, A.G. 1975. Physiological aspects of deep sea biology.In: Monographs of the Physiological Society, No. 31. Cambridge University Press, Cambridge
Mullins, L.J. 1959. The penetration of some cations into muscle.J. Gen. Physiol. 42:817–829
Podolsky, R.J. 1956. A mechanism for the effect of hydrostatic pressure on biological systems.J. Physiol. (London) 132:33P-39P
Roberts, D.V. 1977. Enzyme Kinetics. Cambridge University Press, Cambridge
Samoilov, O.Ya. 1957a. A new approach to the study of hydration of ions in aqueous solutions.Discuss. Faraday Soc. 24:141–146, 216
Samoilov, O.Ya. 1957b. Structure of Aqueous Electrolyte Solutions and Ion Hydration. Izd. A.N.S.S.S.R., Moscow
Samoilov, O.Ya. 1961. Die Struktur von wässrigen Elektrolytlösungen. B.G. Teubner, Leipzig
Schatzmann, H.J. 1953. Herzglykoside als Hemmstoffe für den Aktiven Kalium- und Natriumtransport durch die Erythrocytenmembran.Helv.Physiol. Pharmacol. Acta 11:346–354
Shrivastav, B.B., Parmentier, J.L. 1980. Hydrostatic pressure as a tool in electrophysiology.In: Molecular Mechanisms of Anesthesia, Progress in Anesthesiology. B.R. Fink, editors. Vol. 2, p. 297. Raven Press, New York
Stewart, G.W., Ellory, J.C., Klein, R.A. 1980. Increased human red cell cation passive permeability below 12°C.Nature (London) 286:403–404
Thorne, P.C.L., Roberts, E.R. 1954. Inorganic Chemistry. (6th ed.) pp. 36–37, 789. Oliver & Boyd, London-Edinburgh
Verrall, R.E. 1973. Infrared spectroscopy of aqueous electrolyte solutions.In: Water. F. Franks, editor. Vol. 3, Ch. 5, p. 211. Plenum Press, New York & London
Wieth, J.O. 1972. The selective ionic permeability of the red cell membrane.In: Oxygen Affinity of Hemoglobin and Red Cell Acid-Base Status. M. Rørth and P. Astrup, editors. p. 265. Alfred Benzon Symposium IV, Munksgaard, Copenhagen
Wiley, J.S. 1977. Genetic abnormalities of cation transport in the human erythrocyte.In: Membrane Transport in Red Cells. J.C. Ellory and V.L. Lew, editors. pp. 337–361. Academic Press, New York
Wiley, J.S., Cooper, R.A. 1974. A furosemide-sensitive cotransport of sodium plus potassium in the human red cell.J. Clin. Invest. 53:745–755
Wiley, J.S., Ellory, J.C., Shuman, M.A., Shaller, C.C., Cooper, R.A. 1975. Characteristics of the membrane defect in the hereditary stomatocytosis syndrome.Blood 46:337–356
Willis, J.S., Ellory, J.C., Becker, J.H. 1978. Na−K pump and Na−K-ATPase: Disparity of their temperature sensitivity.Am. J. Physiol. 235:c159-c167
Zimmermann, U., Pilwat, G., Péqueux, A., Gilles, R. 1980. Electromechanical properties of human erythrocyte membranes: The pressure-dependence of potassium permeability.J. Membrane Biol. 54:103–113
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Hall, A.C., Ellory, J.C. & Klein, R.A. Pressure and temperature effects on human red cell cation transport. J. Membrain Biol. 68, 47–56 (1982). https://doi.org/10.1007/BF01872253
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DOI: https://doi.org/10.1007/BF01872253