Abstract
Chicks were fed diets containing 0, 1, 5 and 10 mg/kg ochratoxin A (OA) over a four-week period to study the effects of OA on growth, weight of internal organs, liver RNA, DNA, protein, and glycogen and serum enzymes. Ochratoxin A depressed the rate of growth and relative weight of the bursa of Fabricius, and increased the relative weights of the liver, kidney, pancreas, and various sections of the gastrointestinal tract, but had no effect on the heart and spleen. Concentrations of liver RNA, DNA and protein were decreased while glycogen was increased. Serum alkaline phosphatase and γ-glutamyl transferase activities, and uric acid and creatinine concentrations were elevated while serum proteins, albumin, phosphorus, potassium, and cholesterol were depressed. The effects of OA were time- and dose-dependent.
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Abramson D, Sinha RN, Mills JT (1980) Mycotoxin and odor formation in moist cereal grain granary storage. Cereal Chem 57:346–351
— (1985) Mycotoxin formation and quality changes in granary-stored corn at 16 and 21% moisture content. Sci Alim 5:653–663
— (1987) Mycotoxin formation in moist 2-row and 6-row barley during granary storage. Mycopathologia 97:179–185
Association of Official Analytical Chemists (1984) Official Methods of Analysis. 14 edn, Association of Official Analytical Chemists, Washington DC, 24.003:431
— (1984) Official Methods of Analysis. 14 edn, Association of Official Analytical Chemists, Washington DC, 26.109:494–496
Boldizsar H, Kozma M (1968) Acta Vet Hung 3:256. Cited from Physiology and Biochemistry of the Domestic Fowl (1871) Bell DJ, Freeman BM (eds) Vol II Academic Press, London
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72:248–254
Ceriotti G (1955) Determination of nucleic acids in animal tissue. J Biol Chem 214:59–70
Chang CF, Huff WE, Hamilton PB (1979) A leucocytopenia induced in chickens by dietary ochratoxin A. Poultry Sci 58:555–558
Cole RJ, Cox RH (1981) Handbook of toxic fungal metabolites. Academic Press, New York, 937 pp
Dwivedi P, Burns RB (1984) Pathology of ochratoxicosis A in young broiler chicks. Res Vet Sci 36:92–103
Giles KW, Myers A (1965) An improved diphenylamine method for estimation of deoxyribonucleic acid. Nature 206:92
Huff WE, Wyatt RD, Tucker TL, Hamilton PB (1974) Ochratoxicosis in the broiler chicken. Poultry Sci 53:1585–1591
Huff WE, Hamilton PB (1974) Nephrotoxicity of ochratoxin A in broiler chicks. Poultry Sci 53:1937 (Abstr)
Huff WE, Wyatt RD, Hamilton PB (1975) Nephrotoxicity of dietary Ochratoxin A in broiler chickens. Appl Microbiol 30:48–51
Huff WE, Chang CF, Warren MF, Hamilton PB (1979) Ochratoxin A induced iron deficiency anemia. Appl Environ Microbiol 37:601–604
Kaneko JJ (1980) Clinical Biochemistry of Domestic Animals, 3rd edn, Academic Press, New York
Kubena LF, Harvey RB, Fletcher OJ, Phillips TD, Mollenhauer HH, Witzel DA, Heidelbaugh ND (1985) Toxicity of ochratoxin A and vanadium to growing chicks. Poultry Sci 64:620–628
Kubena LF, Harvey RB, Phillips TD, Fletcher OJ (1986) Influence of ochratoxin A and vanadium on various parameters in growing chicks. Poultry Sci 65:1671–1678
Liker B, Rupic V, Bogdanic C, Balzer I, Muzic S, Herceg M (1978) Influence of ochratoxin A on the activity of alkaline phosphatase, sorbitol dehydrogenase and glutamate dehydrogenase in chick blood plasma. Veterinarski Arkhiv 48: 23–32
Manning RO, Wyatt RD (1984) Toxicity ofAspergillus ochraceus contaminated wheat and different chemical forms of ochratoxin A in broiler chickens. Poultry Sci 63:458–465
Osborne DJ, Huff WE, Hamilton PB, Burmeister HR (1982) Comparison of ochratoxin, aflatoxin, and T-2 toxin for their effects on selected parameters related to digestion and evidence of specific metabolism of carotenoids in chickens. Poultry Sci 61:1646–1652
Prior MG, Sisodia CS (1978) Ochratoxicosis in white leghorn hens. Poultry Sci 57:619–623
SAS (1982) User's guide, Statistics. SAS Inst Inc, Cary, NC
Schneider WC, Hogeboom GM, Ross HE (1950) Intracellular distribution of enzymes VII. The distribution of nucleic acid and adenosine triphosphate in normal mouse liver and mouse hepatoma. J Nat Cancer Inst 10:977–982
Seifter S, Dayton S, Novic B, Muntwyler E (1950) The estimation of glycogen with the anthrone reagent. Arch Biochem 25:191–200
Sreemannarayana O, Frohlich AA, Marquardt RR (1987) Acute toxicity of sterigmatocystin to chicks. Mycopathologia 97:51–59
Swendsen C, Skadhauge E (1976) Renal function in hens fed graded dietary levels of ochratoxin A. Acta Pharmacol Toxicol 38:186–194
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Sreemannarayana, O., Marquardt, R.R., Frohlich, A.A. et al. Organ weights, liver constituents, and serum components in growing chicks fed ochratoxin A. Arch. Environ. Contam. Toxicol. 18, 404–410 (1989). https://doi.org/10.1007/BF01062365
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DOI: https://doi.org/10.1007/BF01062365