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Combined effects of tri-n-butyl tin (TBT) and diuron on marine periphyton communities detected as pollution-induced community tolerance

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Abstract

The effects of combined toxicity were studied, using marine periphyton communities exposed to mixtures of tri-n-butyl tin (TBT) and diuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea, DCMU) in indoor aquaria during four weeks. The experimental design of the study followed a central composite design (CCD) and utilized dose-response surface methodology for evaluation of the results. The detection of pollution-induced community tolerance (PICT) was accomplished by short-term (1 h) tests on inhibition of photosynthesis. Both single-toxicant and two-toxicant short-term tests were used. Two tentative measures of tolerance are proposed to achieve convenient comparisons of the tolerances from the two-toxicant tests. With the detection of PICT, effects of the long-term exposure were recorded on diatom species richness, chlorophyll a accumulation and copepod abundance. The decrease of diatom species richness was accompanied by an increased tolerance (PICT), which was detectable by all tolerance measures used. Primary effects on microalgae were recorded as a decrease in chlorophyll a at higher toxicant concentrations. At lower concentrations, primary effects on copepods were found, which resulted in reduced grazing and increased chlorophyll a content.

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References

  • Alabaster JS, Lloyd R (eds) (1982) Water quality criteria for freshwater fish, 2nd ed. Butterworths, London

    Google Scholar 

  • Aldridge WN, Street BW, Skilleter DN (1977) Halide-dependent and halide-independent effects of triorganotin and triorganolead compounds on mitochondrial functions. Biochem J 168:353–364

    Google Scholar 

  • Bayne CK, Rubin IB (1986) Practical experimental designs and optimization methods for chemists. VCH Publishers, NY, Weinheim

    Google Scholar 

  • Ben-Shlomo R, Nevo E (1988) Isozyme polymorphism as monitoring of marine environments: The interactive effect of cadmium and mercury pollution on the shrimp, Palaemon elegans. Mar Pollut Bull 19:314–317

    Google Scholar 

  • Blanck H (1984) Species dependent variation among organisms in their sensitivity to chemicals. Ecol Bull 36:107–119

    Google Scholar 

  • Blanck H, Wängberg SÅ (1988a) The validity of an ecotoxicological test system. Short-term and long-term effects of arsenate on marine periphyton communities in laboratory systems. Can J Fish Aquat Sci 45:1807–1815

    Google Scholar 

  • —, — (1988b) Induced community tolerance in marine periphyton established under arsenate stress. Can J Fish Aquat Sci 45:1816–1819

    Google Scholar 

  • --, -- (1991) Pattern of cotolerance in marine periphyton communities estabished under arsenate stress. Aquat Toxicol (in press)

  • Blanck H, Wängberg SÅ, Molander S (1988) Pollution-induced community tolerance—a new ecotoxicological tool. In: Cairns J Jr, Pratt JR (eds) Functional testing of aquatic biota for estimating hazard of chemicals, ASTM STP 988. American Society for Testing and Materials, Philadelphia, pp 219–230

    Google Scholar 

  • Box GEP, Hunter WG, Hunter JS (1978) Statistics for experimenters, John Wiley & Sons, NY

    Google Scholar 

  • Brattsten LB, Holyoke CW, Jr., Leeper JR, Raffa KF (1986) Insecticide resistance: challenge to pest management and basic research. Science 231:1255–1260

    Google Scholar 

  • Cairns J Jr, Pratt JR (1989) The scientific basis of bioassays. Hydrobiologia 188/189:5–20

    Google Scholar 

  • Cairns J Jr, Niederlehner BR, Pratt JR (1990) Evaluation of joint toxicity of chlorine and ammonia to aquatic communities. Aquat Toxicol 16:87–100

    Google Scholar 

  • Clarke KR, Green RH (1988) Statistical design and analysis for a biological effects' study. Mar Ecol Prog Ser 46:213–226

    Google Scholar 

  • Dahl B, Blanck H (1990) Pollution-induced community tolerance (PICT) to tributyltin (TBT) in marine periphyton—field study and microcosm experiment. Proc 3rd Int Organotin Symp. Monaco April 17–20, 1990

  • Deneer JW, Sinnige TL, Seinen W, Hermens JLM (1988) The joint acute toxicity to Daphnia magna of industrial organic chemicals at low concentrations. Aquat Toxicol 12:33–38

    Google Scholar 

  • Filbin GJ, Hough RA (1984) Extraction of 14C-labeled photosynthate from aquatic plants with dimethyl sulfoxide (DMSO). Limnol Oceanogr 29:426–428

    Google Scholar 

  • Granmo Å, Kollberg SO (1972) A new simple water flow system for accurate continuous flow tests. Water Res 6:1597–1599

    Google Scholar 

  • Hansson O, Wydrzynski T (1990) Current Perceptions of Photosystem-II. Photosynth Res 23:131–162

    Google Scholar 

  • Hermens J, Leeuwangh P (1982) Joint toxicity of mixtures of 8 and 24 chemicals to the guppy (Poecilia reticulata). Ecotoxicol Environ Safety 6:302–310

    Google Scholar 

  • Hiscox JD, Israelstam F (1978) A method for the extraction of chlorophyll from leaf tissue without maceration. Can J Bot 57:1332–1334.

    Google Scholar 

  • Izawa S (1977) Inhibitors of electron transport. Encyclopedia of Plant Physiology V. Photosynthesis 1, pp 266–282

  • Jeffrey SW, Humphrey GF (1975) New spectrophotometric equations for determining chlorophylls a, b, c 1 and c 2 in higher plants, algae and natural plankton. Biochim Physiol Pflanzen (BPP) 167:191–194

    Google Scholar 

  • Klerks PL, Levinton JS (1989) Effects of heavy metals in a polluted aquatic ecosystem. In: Levine SA, Harwell MA, Kelly JR, Kimball KD (eds) Ecotoxicology—Problems and Approaches, Springer-Verlag, NY, pp 41–67

    Google Scholar 

  • Könemann H (1981) Fish toxicity tests with mixtures of more than two chemicals: a proposal for a quantitative approach and experimental results. Toxicology 19:229–238

    Google Scholar 

  • Luoma SN (1977) Detection of trace contaminant effects in aquatic ecosystems. J Fish Res Board Can 34:436–439

    Google Scholar 

  • Molander S, Blanck H (1991) Detection of pollution-induced community tolerance (PICT) in marine periphyton communities established under diuron exposure. Aquat Toxicol (in press)

  • Molander S, Blanck H, Söderström M (1990) Toxicity assessment by pollution-induced community tolerance (PICT), and identification of metabolites in periphyton communities after exposure to 4,5,6-trichloroguaiacol. Aquat Toxicol 18:115–136

    Google Scholar 

  • Okkerman PC, van der Plassche EJ, Sloof W, Van Leeuwen CJ, Canton JH (1991) Ecotoxicological effects assessment: A comparison of several extrapolation procedures. Ecotoxicol Environ Safety 21:182–193

    Google Scholar 

  • Pitelka LF (1988) Evolutionary response of plants to anthropogenic pollutants. Trends Ecol Evol 3:233–236

    Google Scholar 

  • Renger G (1986) Herbicide interaction with photosystem II: recent developments. Physiol Veg 24:509–521

    Google Scholar 

  • Shoaf WT, Lium BW (1976) Improved extraction of chlorophyll a and b from algae using dimethyl sulfoxide. Limnol Oceanog 21:926–928

    Google Scholar 

  • Sprague JB (1970) Measurement of pollutant toxicity to fish. II. Utilizing and applying bioassay results. Water Res 4:3–32

    Google Scholar 

  • Vouk VB, Butler GC, Upton AC, Parke DV, Asher SC (eds) (1987) Methods for assessing the effects of mixtures of chemicals, SCOPE 30, SGOMSEC 3, John Wiley & Sons, Chichester

    Google Scholar 

  • Wängberg SÅ, Heyman U, Blanck H (1991) Long-term and short-term arsenate toxicity to freshwater phytoplankton and periphyton in limnocorrals. Can J Fish Aquat Sci 48:173–182

    Google Scholar 

  • Watling-Payne AS, Selwyn MJ (1974) Inhibition and uncoupling of photophosphorylation in isolated chloroplasts by organotin, organomercury and diphenyleneiodonium compounds. Biochem J 142:65–74

    Google Scholar 

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Molander, S., Dahl, B., Blanck, H. et al. Combined effects of tri-n-butyl tin (TBT) and diuron on marine periphyton communities detected as pollution-induced community tolerance. Arch. Environ. Contam. Toxicol. 22, 419–427 (1992). https://doi.org/10.1007/BF00212562

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  • DOI: https://doi.org/10.1007/BF00212562

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