Abstract
The OCT plasmid from Pseudomonas maltophilia N246-1 was transferred to Rhodopseudomonas sphaeroides M1 with very low frequency (1.4–1.9 × 10−5 per recipient cell at pH 7–8 for a 3-hour reaction time). P. maltophilia N246-1 was able to utilize C8-C14 of n-alkanes, whereas R. gas-liquid chromatography determined that the broad range of carbon numbers of n-alkanes in crude oil was remarkably degraded by the transconjugant, R. sphaeroides M1-C1, compared with donor strain N246-1. The fact that donor and transconjugant strains simultaneously lost the capacity to utilize n-alkanes on L-broth medium suggests that the OCT plasmids are unstable. It was found that the OCT plasmid of P. maltophilia N246 was incompatible with the IncP-2 group of P. aeruginosa KCTC 11245.
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Offprint requests to: K.-H. Min, Sookmyung Women's University.
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Lee, M.H., Hwang, M.O., Choi, S.Y. et al. n-Alkane dissimilation by Rhodopseudomonas sphaeroides transferred OCT plasmid. Microb Ecol 26, 219–226 (1993). https://doi.org/10.1007/BF00176954
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DOI: https://doi.org/10.1007/BF00176954