Skip to main content
SpringerLink
Log in

Effects of sudden temperature shifts on pure cultures of four strains of freshwater bacteria

  • Published:
Microbial Ecology Aims and scope Submit manuscript

Abstract

Three psychrotrophic and one mesophilic strains were isolated from winter water samples of different freshwater biotopes and identified asCytophaga johnsonae (C-21),Cytophaga sp. (M-17),Pseudomonas fluorescens (KD), andEnterobacter cloacae (BS-2). Temperature shift-up experiments with emphasis on low temperatures were carried out with aerated pure batch cultures in glucose mineral medium. The effects of sudden temperature increases on growth rates and substrate conversion were investigated. All three psychrotrophic strains in the temperature increase experiments at low temperatures showed differing reactions within the linear zone of the Arrhenius plot. TheC. johnsonae (C-21) shift-up cultures adjusted the growth rate immediately to the rate of the temperature adapted cultures, whereasCytophaga sp. (M-17) shift-up cultures showed a lower andP. fluorescens (KD) a higher growth rate. The mesophilicE. cloacae (BS-2), likeC. johnsonae (C-21), adjusted immediately to the new growth rate. Substrate conversion increased in all experiments immediately after the shift-up. The extracellular substrate conversion byP. fluorescens (KD) of glucose to gluconate and 2-ketogluconate was particularly affected by the sudden temperature increase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Allen, M. M.: Simple conditions for growth of unicellular blue-green algae on plates, J. Phycol.4, 1–4 (1968)

    Google Scholar 

  2. Allwood, M. C., and A. D. Russell: Mechanisms of thermal injury in nonsporulating bacteria. Adv. Appl. Microbiol.12, 89–119 (1970)

    PubMed  Google Scholar 

  3. Baig, I. A., and J. W. Hopton: Psychrophilic properties and the temperature characteristic of growth of bacteria. J. Bacteriol.100, 552–553 (1969)

    PubMed  Google Scholar 

  4. Bergmeyer, H. U.: Methoden der enzymatischen Analyse. 3. Auflage, Weinheim: Verlag Chemie (1974)

    Google Scholar 

  5. Brock, T. D., F. Passman, and I. Yonder. Absence of obligately psychrophilic bacteria in constantly cold springs associated with caves in southern Indiana. Am. Midland Naturalist90, 240–246 (1973)

    Google Scholar 

  6. Collins, V. G., and L. G. Willoughby: The distribution of bacteria and fungal spores in Blelham Tarn with particular reference to an experimental overturn. Arch. Mikrobiol.43, 294–307 (1962)

    PubMed  Google Scholar 

  7. Harder, W., and H. Veldkamp: Competition of marine psychrophilic bacteria at low temperature. Antonie Van Leeuwenhoek37, 51–63 (1971)

    PubMed  Google Scholar 

  8. Herbert, D., P. J. Phipps, and R. E. Strange: Chemical analysis of microbial cells. Meth. Microbiol.5B, 209–344 (1971)

    Google Scholar 

  9. Inniss, W. E.: Interaction of temperature and psychrophilic microorganisms. Annu. Rev. Microbiol.29, 445–465 (1975)

    PubMed  Google Scholar 

  10. Lanning, M. C., and S. S. Cohen: The detection and estimation of 2-ketohexonic acids. J. Biol. Chem.189, 109–114 (1951)

    PubMed  Google Scholar 

  11. Lynch, W. H., J. MacLeod, and M. Franklin: Effect of growth temperature on the accumulation of glucose-oxidation products inPseudomonas fluorescens. Can. J. Microbiol.21, 1553–1559 (1975)

    PubMed  Google Scholar 

  12. Morita, R. Y.: Psychrophilic bacteria. Bacteriol. Rev.39, 144–167 (1975)

    PubMed  Google Scholar 

  13. Morita, R. Y.: Survival of bacteria in cold and moderate hydrostatic pressure environments with special reference to psychrophilic and barophilic bacteria. The Survival of Vegetative Microbes, pp. 279–298. Cambridge University Press, Cambridge (1976)

    Google Scholar 

  14. Ng, H., J. L. Ingraham, and A. G. Marr: Damage and derepression inE. coli resulting from growth at low temperatures. J. Bacteriol.84, 331–339 (1962)

    PubMed  Google Scholar 

  15. Palumbo, S. A., and L. D. Witter: The influence of temperature on the pathways of glucose catabolism ofPseudomonas fluorescens, Can. J. Microbiol.15, 995–1000 (1969)

    PubMed  Google Scholar 

  16. Ryu, D. Y., and R. I. Mateles: Transient response of continuous cultures to changes in temperature. Biotechnol. Bioeng.10, 385–397 (1968)

    Google Scholar 

  17. Shaw, M. K.: Effect of abrupt temperature shift on the growth of mesophilic and psychrophilic yeasts. J. Bacteriol.93, 1332–1336 (1967)

    PubMed  Google Scholar 

  18. Topiwala, H., and G. C. Sinclair: Temperature relationship in continuous culture. Biotechnol. Bioeng.13, 795–813 (1971)

    PubMed  Google Scholar 

  19. Welker, N. E.: Microbial endurance and resistance to heat stress. The Survival of Vegetative Microbes, pp. 241–277. Cambridge University Press, Cambridge (1976)

    Google Scholar 

  20. Whiting, P. H., M. Midgley, and E. A. Dawes: The role of glucose limitation in the regulation of the transport of glucose, gluconate and 2-oxogluconate, and of glucose metabolism inPseudomonas aeruginosa. J. Gen. Microbiol.92, 304–310 (1976)

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Limnological Institute, Konstanz-Egg, West Germany

    Manfred G. Höfle

Authors
  1. Manfred G. Höfle
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Höfle, M.G. Effects of sudden temperature shifts on pure cultures of four strains of freshwater bacteria. Microb Ecol 5, 17–26 (1979). https://doi.org/10.1007/BF02010574

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02010574

Keywords

Search

Navigation