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Comparison of agar dilution test, broth dilution test, and broth elution test for assaying susceptibility ofCandida spp

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Abstract

Biochemically and morphologically defined isolates ofCandida spp. were tested for their susceptibility to systemic antimycotics (5-flucytosine, ketoconazole, and miconazole). Three different susceptibility test techniques—the broth disc elution test, the agar dilution test, and the broth dilution test—were examined. The different assays were correlated with the broth dilution test at breakpoint concentrations. Reliability and practicability of the test systems described here were good, and the agreement with the broth dilution test was excellent. The elution test seems to be a valuable method for smaller laboratories, whereas the agar dilution test with antimycotic tablets is a particularly and comparatively inexpensive test for laboratories handling larger numbers of specimens.

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Authors and Affiliations

  1. Center of Hygiene, Johann-Wolfgang-von-Goethe University, D-6000, Frankfurt 70, FRG

    Roland Werk & Hans Knothe

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  1. Roland Werk
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  2. Hans Knothe
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Werk, R., Knothe, H. Comparison of agar dilution test, broth dilution test, and broth elution test for assaying susceptibility ofCandida spp. Current Microbiology 10, 173–175 (1984). https://doi.org/10.1007/BF01576781

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  • DOI: https://doi.org/10.1007/BF01576781

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