Abstract
An enzyme has been isolated fromPseudomonas marginalis (a pathogen responsible for the spoilage of fruits and vegetables in storage) that degrades the exopolysaccharide (mw>2×106 Da) produced by this organism. The mechanism of degradation has been determined to be a depolymerization that results from glucosidic cleavage. The product of depolymerization is a polysaccharide of ca. 2.5×105 Da. The enzyme has a molecular weight of approximately 28 kDa, a pH optimum of 6.5, pI=9.1, and an apparent Km of 1.95×10−7±3.5×10−8 M. Native and enzyme-treated marginalan supported no or very limited growth of the bacterium respectively.
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Osman, S.F., Fett, W.F., Irwin, P.L. et al. Isolation and characterization of an exopolysaccharide depolymerase fromPseudomonas marginalis HT041B. Current Microbiology 26, 299–304 (1993). https://doi.org/10.1007/BF01575921
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DOI: https://doi.org/10.1007/BF01575921