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In vivo phosphorylation of isocitrate lyase inEscherichia coli andAcinetobacter calcoaceticus

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Abstract

Isocitrate lyase inEscherichia coli and inAcinetobacter calcoaceticus is phosphorylated when the cells are grown with acetate as the sole carbon source in low-phosphate mineral salts medium containing32P inorganic phosphate. The level of32P incorporation into the enzyme in both microorganisms appears to be constant throughout the entire growth cycle. Further, theresults of immunoblots and rocket immunoelectrophoresis suggest that the amount of isocitrate lyase protein, although at different levels in each microorganism, also remains constant throughout the growth cycle.

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Literature Cited

  1. Garnak M, Reeves HC (1979) Purification and properties of phosphorylated isocitrate dehydrogenase ofEscherichia coli. J Biol Chem 254:7915–7920

    PubMed  Google Scholar 

  2. Hoyt JC, Reeves HC (1988) In vivo phosphorylation of isocitrate lyase fromEscherichia coli D5H3G7. Biochem Biophys Res Commun 153:875–880

    PubMed  Google Scholar 

  3. Hoyt JC, Reeves HC (1992) Phosphorylation ofAcinetobacter isocitrate lyase. Biochem biophys Res Commun 182:367–371

    PubMed  Google Scholar 

  4. Kunce CM, Trelease RN (1986) Heterogeneity of catalase in maturing and germinating cotton seeds. Plant Physiol 81:1134–1139

    Google Scholar 

  5. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685

    Google Scholar 

  6. Reeves HC, O'Neil S, Weitzman PDJ (1983) Modulation of isocitrate dehydrogenase activity inAcinetobacter calcoaeeticus by acetate. FEBS Lett 163:265–268

    PubMed  Google Scholar 

  7. Reeves HC, O'Neil S, Weitzman PDJ (1986) Changes in NADP-isocitrate dehydrogenase isozymes inAcinetobacter calcoaceticus in response to acetate. FEMS Microbiol Lett 35:229–232

    Google Scholar 

  8. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354

    PubMed  Google Scholar 

  9. Zarembinski CM (1990) Comparison of isocitrale lyase fromEscherichia coli K12 grown on different carbon sources. Master's Thesis, Arizona State University, Tempe, Arizona

    Google Scholar 

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  1. J. C. Hoyt

    Present address: Department of Chemistry, Rice University, 77251, Houston, TX, USA

Authors and Affiliations

  1. Department of Microbiology, Arizona State University, 85287-2701, Tempe, Arizona, USA

    J. C. Hoyt,  H. -P. P. Lin & H. C. Reeves

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  1. J. C. Hoyt
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  2. H. -P. P. Lin
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  3. H. C. Reeves
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Hoyt, J.C., Lin, H.P.P. & Reeves, H.C. In vivo phosphorylation of isocitrate lyase inEscherichia coli andAcinetobacter calcoaceticus . Current Microbiology 28, 67–69 (1994). https://doi.org/10.1007/BF01569048

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