Summary
The proportion of ganglioside GD3 increases in glioma tissue and GD3 content is correlated with malignancy of gliomas. This ganglioside can be detected in the sera of patients with glioma by thin-layer chromatographic analysis.
Ganglioside GD3 was not detected in the sera of healthy donors and astrocytoma grade 2 patients. However, serum GD3 was detected in one of three astrocytoma grade 3 patients and seven of nine glioblastoma patients. These results show that shedding of GD3 increases in proportion to the degree of malignancy of gliomas. Nevertheless, all of the glioblastoma patients in this study were advanced cases. Considering the high reliability of radiological diagnostic techniques in the neurosurgical field, further study will be necessary to clarify the relationships between the GD3 level in serum and the properties of tumours.
Similar content being viewed by others
References
Bernhard H, Meyer Zum Buschenfelde KH, Dippold WG (1989) Ganglioside GD3 shedding by human malignant melanoma cells. Int J Cancer 44: 155–160
Dippold W, Knuth A, Meyer Zum Buschenfelde KH (1984) Inhabitation of human melanoma cell growth in vitro by monoclonal anti-GD3-ganglioside antibody. Cancer Res 44: 806–810
Eto Y, Shinoda S (1982) Gangliosides and neutral glycosphingolipids in human brain tumors: specificity and their significance. Advances in Experimental Medicine and Biology 152: 279–280
Hoon DB, Irie RF, Cochran AJ (1988) Gangliosides from human melanoma immunomodulate response of T cells to interleukin-2. Cell Immunol 111: 410–419
Houghton AL, Mintzer D, Cordon-Cardo C, Welt S, Fliegel B, Vadhan S, Carswell E, Melamed MR, Oettgen HF, Old LJ (1985) Mouse monoclonal IgG3 antibody detecting GD3 ganglioside: A Phase 1 trial in patients with malignant melanoma. Proc Natl Acad Sci USA 82: 1242–1246
Ladisch S, Gillard B, Wong C, Ulsh L (1983) Shedding and immunoregulatory activity of YAC-1 lymphoma cell gangliosides. Cancer Res 43: 3808–3813
Ladisch S, Wu Z, Frig S, Ulsh L, Schwartz E, Floutsis G, Wiley F, Lenars C, Seeger R (1987) Shedding of GD2 ganglioside by human neuroblastoma. Int J Cancer 39: 73–76
Lo HS, Hogan EL, Koontz DA, Traylor TD (1980) Serum gangliosides in cerebral astrocytoma. Ann Neurol 8: 534–538
Nakamura O, Ishihara E, Iwamori M, Nagai Y, Matsutani M, Nomura K, Takakura K (1987) Lipid composition of human malignant brain tumors. Brain and Nerve 39: 221–226
Portoukalian J, David MJ, Shen X, Richard M, Dubreuil C (1989) Tumor size-dependent elevations of serum gangliosides in patients with head and neck. Biochem Int 18: 759–765
Seyfried TN, Yu RK (1985) Ganglioside GD3: structure, cellular distribution and possible function. Mol Cell Biochem 68: 3–10
Traylor TD, Hogan EL (1980) Gangliosides of human cerebral astrocytomas. J Neurochem 34: 126–131
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nakamura, O., Iwamori, M., Matsutani, M. et al. Ganglioside GD3 shedding by human gliomas. Acta neurochir 109, 34–36 (1991). https://doi.org/10.1007/BF01405694
Issue Date:
DOI: https://doi.org/10.1007/BF01405694