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Distribution of mouse interferon-β in normal and brain tumour-bearing mice

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Summary

The distribution of125I-labelled recombinant mouse interferon-β (rMuIFN-β) in normal and glioma (203 glioma) bearing mice was studied by radioassay and macro-autoradiography at 15 and 30 min after a single intravenous injection. The level of rMuIFN-β in the spleen was about 20-fold higher than in serum. Concentrations higher than the serum level was detected in the lung, liver and kidney. The concentration of rMuIFN-β in the brain was 8% of the serum level and the concentration in the glioma 30 min after administration was about 10-fold higher than in normal mouse brain. Macro-autoradiographic study demonstrated a wide distribution range and selective uptake in glioma tissue. Furthermore, we found that mouse gliomas were sensitive to mouse IFN-β. Our findings demonstrate that in the mouse glioma model, intravenously administered interferon reaches the tumour.

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Authors and Affiliations

  1. Department of Neurosurgery, Kumamoto University Medical School, Kumamoto

    Y. Mihara, J. Kuratsu, S. Takaki & Y. Ushio

  2. Metabolism Research Section, Tokai Research Laboratories, Daiichi Pure Chemicals Co., Ltd., Ibaraki

    K. Hori

  3. Daiichi Seiyaku Co., Ltd., Tokyo

    E. Nagai

  4. Pharmaceutical Operations Department, Toray Industries, Tokyo, Japan

    Y. Satoh & N. Minowa

Authors
  1. Y. Mihara
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  2. J. Kuratsu
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  3. S. Takaki
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  4. K. Hori
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  5. E. Nagai
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  6. Y. Satoh
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  7. N. Minowa
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  8. Y. Ushio
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Mihara, Y., Kuratsu, J., Takaki, S. et al. Distribution of mouse interferon-β in normal and brain tumour-bearing mice. Acta neurochir 109, 46–51 (1991). https://doi.org/10.1007/BF01405697

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