Summary
Changes of plasma thromboxane level in subarachnoid haemorrhage (SAH) were studied clinically and experimentally using 11-dehydro-thromboxane B2 (11 DTX) as a measuring index. 11 DTX is a major long-lived metabolite formed from thromboxane (TX) B2, and is said to be a more reliable parameter for detecting TXA2 production in biological systems.
In this clinical study, blood was sampled from the cubital vein of 10 SAH patients on the earliest possible day (day 0 or 1), during the vasospasm predilection period (day 7∼11) and in the chronic stage (day 16∼32). Plasma concentrations of 11 DTX and 6-keto-1α were measured in clinical cases.
A canine SAH model was produced by the two haemorrhage methods and blood was sampled from the superior sagittal sinus before and on day 4 of the first cisternal blood injection. 11 DTX, TXB2 and platelet function were examined in each sample.
In the clinical studies, plasma 11 DTX levels tended to be higher in the early stage of SAH but decreased thereafter to the normal or lower level. Plasma concentrations of 6-keto-α tended to decrease mildly during the vasospasm predilection period.
In the experimental study, neither definite change of plasma 11 DTX level nor neurological deficit could be induced by the mimic SAH, while an increase in platelet aggregability and narrowing of the basilar artery were observed. 11 DTX was inferred to be a more reliable parameter of TX biosynthesis than TXB2.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baena RR, Gaetani P. Grignani G, Pacchiarini L, Vigano T, Scalabrini DR, Silvani V, Folco G, Paoletti P (1987) Role of arachidonate metabolites in the genesis of cerebral vasospasm. ADV Prostaglandin thromboxane Leukotrien Res 17: 938–942
Blair IA, Barrow SE, Waddell KA, Lewis PJ, Dollery CT (1982) Prostacyclin is not a circulating hormone in man. Prostaglandins 23: 579–589
Catella F, Healy D, Lawson JA, FitzGerald GA (1986) 11-dehydrothromboxane B2: A quantitative index of thromboxane A2 formation in the human circulation. Proc Natl Acad Sci 83: 5861–5865
Granström E, Kumlin M (1987) Assay of thromboxane production in biological systems: Reliability of TXB2 versus 11-Dehydro-TXB2 as targets for measurement. ADV Prostaglandin Thromboxane Leukotrien Res 17: 587–594
Hasegawa T (1980) Studies of intravascular components with cerebral vasospasm following subarachnoid haemorrhage with particular regard to platelet and blood coagulation functions. Neurol Med Chir (Tokyo) 20: 473–480
Kawano K, Sugita M, Fukukawa T, Tabata N, Yamamoto K, Hirai A, Tamura Y, Yoshida S (1991) A simple and rapid extraction on 11-dehydro-thromboxane B2. Jpn J Inf 11: 59–63
Lawson JA, Patrono C, Ciabattoni G, FitzGerald GA (1986) Long-lived enzymatic metabolites of thromboxane B2 in the human circulation. Anal Biochem 155: 198–205
Maeda Y, Tani E, Miyamoto T (1981) Prostaglandin metabolism in experimental cerebral vasospasm. J Neurosurg 55: 779–785
Mitsuka S. Nukui H, Sasaki H. Toyoda O, Horikoshi S (1985) Changes of blood coagulation function in experimental vasospasm of dog. Jpn J Stroke 7: 150–157
Nonogaki Y, Suzuki S, Ohkuma H, Souma M, Ebina K, Iwabuchi T (1988) Changes of plasma 11-dehydro-TXB2 and TXB2 by tourniquet ischemia. Hirosaki Med J 42: 156–161
Ohkuma H (1988) Role of platelet function in symptomatic cerebral vasospasm. Jpn J Stroke 10: 355–363
Ohmoto T (1984) Cerebral vasospasm and prostaglandins. Neurol Surg (Tokyo) 12: 1453–1464
Patrono C, Ciabattoni G (1986) Estimated rate of thromboxane secretion into the circulation of normal humans. J Clin Invest 77: 590–594
Pickard JD, Walker V, Vile J, Perry S, Smythe PJ, Hunt R (1987) Oral nimodipine reduces prostaglandin and thromboxane production by arteries chronically exposed to a periarterial haematoma and the antifibrinolytic agent tranexamic acid. J Neurol Neurosurg Psychiatry 50: 727–731
Sasaki T, Murota S, Wakai S, Asano T, Sano K (1981) Evaluation of prostaglandin biosynthetic activity in canine basilar artery following subarachnoid injection of blood. J Neurosurg 55: 771–778
Seifert V, Stolke D, Kaever V, Dietz H (1987) Arachidonic acid metabolism following aneurysm rupture. Evaluation of cerebrospinal fluid and serum concentration of 6-keto-prostaglandin F1α and thromboxane B2 in patients with subarachnoid haemorrhage. Surg Neurol 27: 243–252
Shinoaga M, Fujii S, Tokoro K, Inomori S, Fujitsu K, Kuwabara T, Tsubone K (1986) Sequential measurements of TXB2 and 6-keto-PGF1α in cerebrospinal fluid and serum in patients with subarachnoid haemorrhage. Neurol Med Chir (Tokyo) 26: 277–283
Suzuki S, Sobata E, Iwabuchi T (1981) Prevention of cerebral ischemic symptoms in cerebral vasospasm with trapidil, an antagonist and selective synthesis inhibitor of thromboxane A2. Neurosurgery 9: 679–685
Suzuki S, Sano K, Handa H, Asano T, Tamura A, Yonekawa Y, Ono H, Tachibana N, Hanaoka K (1989) Clinical study of OKY-046, thromboxane synthetase inhibitor, in prevention of cerebral vasospasm and delayed cerebral ischaemic symptoms after subarachnoid haemorrhage due to aneurysmal rupture: a randomized double-blind study. Neurol Res 11: 79–88
Suzuki S, Suzuki M, Iwabuchi T (1983) Pole of multiple cerebral microthrombosis in symptomatic cerebral vasospasm: with a case report. Neurosurgery 13: 199–203
Suzuki S, Kimura M, Souma M, Ohkuma H, Shimizu T, Iwabuchi T (1990) Cerebral microthrombosis in symptomatic cerebral vasospasm—A quantitative histopathological study in autopsy cases-. Neurol Med Chir (Tokyo) 30: 627–634
Westlund P, Kumlin M, Nordenström A, Granström E (1986) Circulating and urinary thromboxane B2 metabolites in the rabbit: 11-dehydro-thromboxane B2 as parameter of thromboxane production. Prostaglandins 3: 413–443
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Suzuki, S., Nonogaki, Y., Ebina, K. et al. Changes of plasma thromboxane level in subarachnoid haemorrhage. Acta neurochir 113, 166–170 (1991). https://doi.org/10.1007/BF01403202
Issue Date:
DOI: https://doi.org/10.1007/BF01403202