Summary
Simian cytomegaloviruses (CMV) isolated from African green monkeys and Japanese macaques were compared serologically and genetically. Twenty-three out of 31 sera from Japanese, Taiwan and rhesus macaques were positive for neutralizing antibodies to simian CMVs isolated from both African green monkeys and a Japanese macaque. The neutralizing antibody titers of those sera were slightly higher to the isolates of African green monkeys origin than to that of Japanese macaque origin. Studies with antisera prepared in guinea pigs revealed a partial antigenic cross-reaction between Japanese macaque CMV (JaM-CMV) and African green monkey CMV (AGM-CMV): the antiserum to JaM-CMV neutralized both homologous JaM-CMV and heterologous AGM-CMV, but the antisera to AGM-CMV neutralized only homologous AGM-CMV.
DNAs of both AGM-CMV and JaM-CMV were compared by restriction endonuclease cleavage analysis. The individual simian CMV isolates showed unique restriction patterns when cleaved withEcoRI,BamHI, orHindIII. In addition, DNA of JaM-CMV was distinguishable from that of AGM-CMV when cleaved withEcoRI orBamHI.
Similar content being viewed by others
References
Asher DM, Gibbs CJ, Lang DJ, Gajdusek DC (1974) Persistent shedding of cytomegalovirus in the urine of healthy rhesus monkeys. Proc Soc Exp Biol Med 145: 794–801
Black PH, Hartley JW, Rowe WP (1963) Isolation of a cytomegalovirus from African green monkey. Proc Soc Exp Biol Med 112: 601–605
Dreesman GR, Benyesh-Melnick M (1967) Spectrum of human cytomegalovirus complement-fixing antigens. J Immunol 99: 1106–1114
Eizuru Y, Inagawa S, Minamishima Y (1984) Application of “Hirt supernatant” DNA to the molecular epidemiology of cytomegalovirus infections. J Clin Microbiol 20: 1012–1014
Eizuru Y, Minamishima Y (1988) Induction of Fc (IgG) receptor(s) by simian cytomegaloviruses in human embryonic lung fibroblasts. Intervirology 29: 339–345
Graham BJ, Minamishima Y, Dreesman GR, Haines HG, Benyesh-Melnick M (1971) Complement-requiring neutralizing antibodies in hyperimmune sera to human cytomegaloviruses. J Immunol 107: 1618–1630
Huang ES, Kilpatrick B, Lakeman A, Alford CA (1978) Genetic analysis of cytomegalovirus-like agent isolated from human brain. J Virol 26: 718–723
Huang ES, Pagano JS (1974) Human cytomegalovirus II. Lack of relatedness to DNA of herpes simplex virus I and II, Epstein-Barr virus, and nonhuman strains of cytomegalovirus. J Virol 13: 642–645
Minamishima Y, Graham BJ, Benyesh-Melnick M (1971) Neutralizing antibodies to cytomegaloviruses in normal simian and human sera. Infect Immun 4: 368–373
Smith KO, Thiel JF, Newman JT, Harvey E, Trousdale MD, Gehle WD, Clark G (1969) Cytomegaloviruses as adventitious contaminants in primary African green monkey kidney cell cultures. J Nat Cancer Inst 42: 489–496
Swack NS, Liu OC, Hsiung GD (1971) Cytomegalovirus infections of monkeys and baboons. Am J Epidemiol 94: 397–402
Swinkels BW, Geelen JLMC, Wertheim-van Dillen P, van Es AA, van der Noordaa J (1984) Initial characterization of four cytomegalovirus strains isolated from chimpanzees. Arch Virol 82: 125–128
Wroblewska Z, Gilden D, Devlin M, Huang ES, Rorke LB, Hamada T, Furukawa T, Cummins L, Kalter S, Koprowski H (1979) Cytomegalovirus isolation from a chimpanzee with acute demyelinating disease after inoculation of multiple sclerosis brain cells. Infect Immun 25: 1008–1015
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Eizuru, Y., Tsuchiya, K., Mori, R. et al. Immunological and molecular comparison of simian cytomegaloviruses isolated from African green monkey (Cercopithecus aethiops) and Japanese macaque (Macaca fuscata). Archives of Virology 107, 65–75 (1989). https://doi.org/10.1007/BF01313879
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01313879