Summary
The cytotropism of two strains, GDVII and DA, of Theiler's murine encephalomyelitis viruses (TMEV) was studied in the oligodendrocyte-enriched murine neural cell cultures. Both GDVII and DA caused cytopathic effects in the neural cell cultures, and double immunostaining for galactocerebroside (Gal-Cer), a marker molecule for oligodendrocyte, and viral antigens disclosed a dual expression of Gal-Cer and viral antigens in over 80% of cells in both cultures 24h after infection with either GDVII or DA. The kinetics of cell-free and cell-associated infectivity were not significantly different between two cultures. These in vitro observations suggest that neither replication in oligodendrocyte nor cell-associated infectivity is a sole factor in discriminating those two subgroups of TMEV with regard to the demyelinating activity, and that virus cell binding may play an important role in virus persistence and TMEV-induced demyelination.
This is a preview of subscription content, log in via an institution to check access.
References
Acharya R, Fry E, Stuart D, Fox G, Rowlands D, Brown F (1989) The three-dimensional structure of foot-and-mouth disease virus at 2.9 Å resolution. Nature 337: 709–716
Friedmann A, Lipton HL (1980) Replication of Theiler's murine encephalomyelitis viruses in BHK21 cells: an electron microscopic study. Virology 101: 389–398
Kim SU, Sato Y, Silberberg DH, Pleasure DE, Rorke LB (1983) Long-term culture of human oligodendrocytes. J Neurol Sci 62: 295–301
Lipton HL (1975) Theiler's virus infection in mice: an unusual biphasic disease process leading to demyelination. Infect Immun 11: 1147–1155
Liu C, Collins J, Sharp E (1967) The pathogenesis of Theiler's GDVII encephalomyelitis virus infection in mice as studied by immunofluorescent technique and infectivity titrations. J Immunol 98: 46–55
Martin A, Wychowski C, Couderc T, Crainic R, Hogle J, Girard M (1988) Engineering a poliovirus type 2 antigenic site on a type 1 capsid results in a chimaeric virus which is neurovirulent for mice. EMBO J 7: 2839–2847
Murray MG, Bradley J, Yang X-F, Wimmer E, Moss EG, Racaniello VR (1988) Poliovirus host range is determined by a short amino acid sequence in neutralization antigenic site I. Science 241: 213–215
Ohara Y, Roos RP (1987) The antibody response in Theiler's virus infection: new perspectives on multiple sclerosis. In: Melnick JL (ed) Progress in medical virology, vol 34. Karger, Basel, 156–179
Ohara Y, Senkowski A, Fu J, Klaman L, Goodall J, Toth M, Roos RP (1988) Trypsin-sensitive neutralization site on VP1 of Theiler's murine encephalomyelitis viruses. J Virol 62: 3527–3529
Ohara Y, Roos RP (1989) Viral infectious complementary-DNA studies may identify nonviral genes critical to central nervous system disease. Ann Neurol 25: 305–309
Rodriguez M, Oleszak E, Leibowitz J (1987) Theiler's murine encephalomyelitis: a model of demyelination and persistence of virus. CRC Crit Rev Immunol 7: 325–365
Stroop WG, Baringer JR, Brahic M (1981) Detection of Theiler's virus RNA in mouse central nervous system by in situ hybridization. Lab Invest 45: 504–509
Theiler M, Gard S (1940) Encephalomyelitis of mice. I. Characteristics and pathogenesis of the virus. J Exp Med 72: 49–67
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ohara, Y., Konno, H., Iwasaki, Y. et al. Cytotropism of Theiler's murine encephalomyelitis viruses in oligodendrocyte-enriched cultures. Archives of Virology 114, 293–298 (1990). https://doi.org/10.1007/BF01310760
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01310760