Summary
A panel of 15 monoclonal antibodies, produced against the hog cholera virus, were characterized by radioimmunoprecipitation assays. Using this panel, we were able to identify 4 sets of monoclonal antibodies precipitating each a different viral protein with relative molecular weight of 40, 46, 120 kDa, respectively, and a protein complex containing 15, 16, 27, and 55 kDa polypeptides which were further characterized. One monoclonal antibody recognized an antigenic determinant at the C-terminal cleavage product of the non-structural p 125 of BVDV. The 40 kDa protein was precipitated from the pelleted virions, indicating its structural importance. On the contrary the 46 kDa protein could only be precipitated from the cell lysate and not from the pelleted virions. The glycosylated 15/16 kDa-55 kDa proteins form a disulfide linked heterodimer on the virus particle with a relative molecular weight of 65 kDa.
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References
Akkina R (1991) Pestivirus bovine viral diarrhea virus polypeptides: identification of new precursor proteins. Virus Res 19: 67–82
Bolin SR (1988) Viral and viral protein specificity of antibodies induced in cows persistently infected with noncytopathic bovine viral diarrhea virus after vaccination with cytopathic bovine viral diarrhea virus. Am J Vet Res 49: 1040–1044
Caij A, De Smet A, Dubois N, Koenen F (1989) High titre Hog Cholera Virus production on CytodexR microcarriers cultures. Arch Virol 105: 113–118
Caij A, Muyldermans G, De Smet A, Hamers R, Koenen F (1993) Production and characterization of monoclonal antibodies against hog cholera virus (Alfort 187 strain). Arch Virol 131: 185–192
Collett MS, Larson R, Gold C, Strick D, Anderson D, Purchio A (1988) Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus. Virology 165: 191–199
Collett MS, Larson R, Belzers SK, Retzel E (1988) Proteins encoded by bovine viral diarrhea virus: the genomic organization of a pestivirus. Virology 165: 200–208
Collett MS, Wiskerchen MA, Welniak E, Belzer SK (1991) Bovine viral diarrhea virus genome organization. In: Liess B, Moennig V, Pohlenz J, Trautwein G (eds) Ruminant pestivirus infections. Arch Virol [Suppl] 3: 19–27
Coria MF, McClurkin AW (1978) Specific immune tolerance in an apparently healthy bull persistently infected with bovine viral diarrhea virus. J Am Vet Med Assoc 172: 449–451
Darbyshire JH (1960) A serological relationship between swine fever and mucosal disease of cattle. Vet Rec 72: 331
Donis RO, Dubovi EJ (1987) Characterization of bovine viral diarrhoea-mucosal virus disease proteins in bovine cells. J Gen Virol 68: 1597–1605
Donis RO, Dubovi EJ (1987) Glycoproteins of bovine viral diarrhoea-mucosal virus disease proteins in bovine cells. J Gen Virol 68: 1607–1616
Greiser-Wilke I, Moennig V, Coulibaly C, Dahle J, Leder L, Liess B (1990) Identification of conserved epitopes on a hog cholera virus protein. Arch Virol 111: 213–225
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T 4. Nature 227: 680–686
Matthaeus W (1979) Detection of three polypeptides in preparations of bovine viral diarrhea virus. Arch Virol 59: 299–305
Meyers G, Rumenapf T, Thiel HJ (1989) Molecular cloning and nucleotide sequence of the genome of hog cholera virus. Virology 171: 555–567
Moennig V (1990) Pestiviruses: a review. Vet Microbiol 23: 35–54
Moormann R, Petra A, Warmerdam M, Van Der Meer B, Schaaper W, Wensvoort G, Hulst M (1990) Molecular cloning and nucleotide sequence of hog cholera virus strain Brescia and mapping of the genomic region encoding envelope protein E 1. Virology 177: 184–198
Pritchett RF, Zee YC (1975) Structural proteins of bovine viral diarrhea virus. Am J Vet Res 36: 1731–1734
Purchio AF, Larson R, Collett MS (1984) Characterization of bovine viral diarrhea viral proteins. J Virol 50: 666–669
Renard A, Dina D, Martial J (1987) Vaccines and diagnostics derived from bovine diarrhea virus. European Patent Application Number 86870095.6. Publication number 0208672, 14 January
Thiel H-J, Stark R, Weiland E, Rümenapf T, Meyers G (1991) Hog cholera virus: molecular composition of virions from a pestivirus. J Virol 65: 4705–4712
Weiland E, Stark R, Haas B, Rumenapf T, Meyers G, Thiel H-J (1990) Pestivirus glycoprotein which induces neutralizing antibodies forms part of a disulfide-linked heterodimer. J Virol 64: 3563–3569
Wensvoort G (1989) Epitopes on structural proteins of hog cholera (swine fever) virus. Thesis, Rijksuniversiteit, Utrecht, Netherlands
Westaway EG, Brinton MA, Gaidamovich SYA, Horzinek MC, Igarashi A, Kääriäinen L, Lvov DK, Porterfield JS, Russell PK, Trent DW (1985) Flaviviridae. Intervirology 24: 125–139
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Muyldermans, G., Caij, A., De Smet, A. et al. Characterization of structural and non-structural proteins of hog cholera virus by means of monoclonal antibodies. Archives of Virology 131, 405–417 (1993). https://doi.org/10.1007/BF01378641
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DOI: https://doi.org/10.1007/BF01378641