Summary
The full length gene for the coat protein (CP) of the potyvirus, Johnsongrass mosaic virus, was incorporated into recombinant baculovirus and expressed in insect cells. Western blot and Coomassie-stained polyacrylamide gel electrophoresis analysis of infected insect cells demonstrated that CP was produced in large quantity. Electron microscopic examination of these cells showed the presence of numerous potyvirus-like particles in the cytoplasm. Morphologically the particles resembled potyvirus particles assembled in vitro in the absence of viral RNA and those found inEscherichia coli expressing the recombinant CP gene.
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References
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Edwards, S.J., Hayden, M.B., Hamilton, R.C. et al. High level production of potyvirus-like particles in insect cells infected with recombinant baculovirus. Archives of Virology 136, 375–380 (1994). https://doi.org/10.1007/BF01321065
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DOI: https://doi.org/10.1007/BF01321065